Inclusion of pepper extract containing capsaicin in the diet of ewes in the mid-lactation period: effects on health, milk production, and quality

Authors

DOI:

https://doi.org/10.33448/rsd-v9i11.10020

Keywords:

Pepper extract; Oxidants; Antioxidants; Health status; Nutrition; Lactation.

Abstract

Pepper extract (PE, 5 g capsaicin/kg PE) was added to the feed of the sheep during the lactation period (Day 75-93) to maintain production, improve milk quality, and preserve their health. The groups were: T0, control, (without PE); T200 (200 mg PE/kg concentrate) and T400 (400 mg PE/kg concentrate). The reduction in milk production (L) was smaller in the T400 ewes on days 0 to 18 and 14 to 18 than in the T0 group. Feed conversion was lower in sheep in groups T200 and T400 than in group T0. The interaction between the treatment and the day for protein, lactose and total milk totals was greater in ewes that consumed PE on day 18. The somatic cell counts in milk were lower in the T400 ewes. The levels of total protein and globulin were the highest in the blood of animals in the T400 group. There were lower levels of reactive oxygen species and lipoperoxidation in the serum and milk of animals in groups T200 and T400. On the 18th day, the serum of sheep that consumed PE increased levels of non-protein thiols and superoxide dismutase activities. The inclusion of PE (400 mg/kg) containing capsaicin in sheep concentrate in the middle of lactation (after the peak of lactation) minimized the reduction in milk production during the experiment and improved the quality of the milk, as well as stimulated an antioxidant response systemic.

References

AOAC, (2000). Official methods of analysis. Gaithersburg: Association of Official Analytical Chemists.

Alba, D., Campigotto, G., Cazarotto, C., Santos, D., Gebert, R., Reis, J., Souza, F. C, Baldissera, M., Gindri, A., Kempka, A., Palmer, E., Vedovatto, M. & Silva, A. S., (2019). Use of grape residue flour in lactating dairy in heart stress: effects on health, milk production and milk quality. Journal of Thermal Biology, 82, 197-205.

Ali, S.F., Lebel, C.P. & Bondy, S.C. F., (1992). Reactive oxygen species formation as a biomarker of methylmercury and trimethyltin neurotoxicity. Neurotocology, 13, 637-648.

Amado, L.L., Garcia, M. L., Ramos, P. B., Freitas, R. F., Zafalon, B., Ferreira, J. L. R., Yunes, J. S. & Monserrat, M. J., (2009). A method to measure total antioxidant capacity against peroxyl radicals in aquatic organisms: Application to evaluate microcystins toxicity. Science of the Total Environment, 407, 2115–2123. https://doi.org/10.1016/j.scitotenv.2008.11.038

An, X., Wang, Y., Wang, R., Hao, X., Hu, Y., Guo, T., Zhang, J., Wang, W., Shi, X., Han, S. & Qi, J., (2020). Effects of a blend of cinnamaldehyde, eugenol and capsicum oleoresin (CEC) on growth performance, nutrient digestibility, immune response and antioxidant status of growing ewes. Livestok Science, 234, 103982. https://doi.org/10.1016/j.livsci.2020.103982

Beutler, E. Red cell metabolism: a manual of biochemical methods, (1984). New York: Grune and Stratton.

Calsamiglia, S., Busquet, M., Cardozo, P. W., Castillejos, L. & Ferret, A., (2007). Invited review: essential oils as modifiers of rumen microbial fermentation. Journal of. Dairy Science, 90, 2580–2595. https://doi.org/10.3168/jds.2006-644

Cannas, A., Nuddas, A. & Pulina, G., (2002). Nutritional strategies to improve lactation persistency in dairy ewes. New York: Cornell University Ithaca.

Cardozo, P. W., Calsamiglia, S., Ferret, A. & Kamel, C., (2006). Effects of alfalfa extract, anise, capsicum and a mixture of cinnamaldehyde and eugenol on ruminal fermentation and protein degradation in beef heifers fed a high concentrate diet. Journal of Animal Science, 84, 2801-2808. https://doi.org/10.2527/jas.2005-593

Castillo, C., Benedito, J. L., Vázquez, P., Pereira, V., Méndez, J., Sotilloc, J. & Hernández, J., (2012). Effects of supplementation with plant extract product containing carvacrol, cinnamaldehyde and capsaicin on serum metabolites and enzymes during the finishing phase of feedlot-fed bull calves. Animal Feed Science and Technology, 171, 246-250. https://doi.org/10.1016/j.anifeedsci.2011.11.006

Choi, S. E.; Kim, T. H.; Yi, S. A., Hwang, Y. C.; Hwang, W. S.; Choe, S. J.; Han, S. J.; Kim, H. J.; Kim, D. J.; Kang, Y. & Lee, K.W., (2011). Capsaicin attenuates palmitateinduced expression of macrophage inflammatory protein 1 and interleukin 8 by increasing palmitate oxidation and reducing c-Jun activation in THP-1 (human acute monocytic leukemia cell) cells. Nutrition Research, 31, 468-478. https://doi.org/10.1016/j.nutres.2011.05.007

Feldman, B.F.; Zinkl, J.G. & Jain, N.C., (2000). Schalm's veterinary hematology. Philadelphia: Lippincott Williams & Wilkins.

Fonseca, J. J. S (2002). Metodologia da pesquisa científica. Fortaleza: UEC, 2002. Apostila.

Franco-Penteado, C. F., Souza, I. A., Lima, C. S., Teixeira, S. A., Muscara, M. N., Nucci, G. & Antunes, E., (2006). Effects of neonatal capsaicin treatment in the neutrophil production, and expression of preprotachykinin-I and tachykinin receptors in the rat bone marrow. Neuroscience Letters, 407, 70-73.

Gerhardt, T. E. & Silveira, D. T., (2009). Métodos de pesquisa. Porto Alegre: Editora da UFRGS.

Gil, Antonio Carlos (2008). Métodos e técnicas de pesquisa social. São Paulo: Atlas.

Jaguezeski, A.M., Gessica, P., Shogor, A.L.B. & Da Silva, A.S., (2018). Addition of curcumin to the diet of dairy sheep improves health, performance and milk quality. Anim. Feed Sci. Technol. 246, 144 – 157.

Materska, M. & Perucka, I., (2005). Antioxidant activity of the main phenolic compounds isolated from hot papper fruit (Capsicum annuum L.). Journal Agricultural Food Chemistry, 53, 1750-1756.

Mates, J. M, Perez-Gomez, C. & Castro, I. N. D., (1999). Antioxidant enzymes and human diseases. Clinical Biochemistry, 32, p. 595-603.

Monserrat, J.M., Geracitano, L. A., Pinho, G. L. L., Vinagre, T. M., Faleiros, M., Alciati, J. C. & Bianchini, A., (2003). Determination of lipid peroxides in invertebrates tissues using the Fe(III) xylenol orange complex formation. Archives of Environmental Contamination and Toxicology, 45, 177–183. https://doi.org/10.1007%2Fs00244-003-0073-x

Neiva, J. N. M., Teixeira, M., Turco, S. H. N., Oliveira, S. M. P. & Moura, A. A. A. N., (2004). Effects of environmental stress on physiological parameters of feedlot sheep in the Northeast of Brazil. Revista Brasileira de Zootecnia, 33, 668-678.

Nunes, A.S., Oliveira R.L., Borja, M.S., Bagaldo, A.R., Macome, F.M., Silva, T.M., Barbosa, L.P., Pires, L. & Fróes, A., (2011). Intake, digestibility and blood parameters of lambs fed diets with palm kernel. Arquivo Brasileiro de Zootecnia, 60, 1-10.

Oh, J., Hristov, A. N., Giallongo, F., Frederick, T., Pate, J., Walusimbi, S., Elia, R. J., Wall, E. H. & Bravo, D., (2015). Effects of dietary Capsicum oleoresin on productivity and immune responses in lactating dairy cows. Journal of Dairy Science, 98, 6327-6339. https://doi.org/10.3168/jds.2014-9294

Oh, J., Harper, M., Giallongo, F., Bravo, D. M., Wall, E. H. & Hristov, A. N., (2017). Effects of rumen-protected Capsicum oleoresin on productivity and responses to a glucose tolerance test in lactating dairy cows. Journal of Dairy Science, 100, 1888-1901. https://doi.org/10.3168/jds.2016-11665

Reyes-Escogido, M. D. L., Gonzalez-Mondragon, E. G. & Vazquez-Tzompantzi, E., (2011). Chemical and pharmacological aspects of capsaicin. Molecules, 16 (2), 1253-1270. https://doi.org/10.3390/molecules16021253

Roberts, K., Shenoy, R. & Anand, P., (2011). A novel human volunteer pain model using contact heat evoked potentials (CHEP) following topical skin application of transient receptor potential agonists capsaicin, menthol and cinnamaldehyde. Journal Clinical Neuroscience, 18, 926-932.

Rodríguez-Prado, M., Ferret, A., Zwieten, J., Gonzalez, L., Bravo, D. & Calsamiglia, S., (2012). Effects of dietary addition of capsicum extract on intake, water consumption, and rumen fermentation of fattening heifers fed a high-concentrate diet. Journal of Animal Science, 90, 1879-1884. https://doi.org/10.2527/jas2010-3191

Santos, D.S., Klauck V., Campigoto G., Alba, D.F., Vedovatto M & Da Silva A.S., (2019). Benefits of the inclusion of açai oil in the diet of dairy sheep in heat stress on health and milk production and quality. Journal of Thermal Biology, 84, 250-258.

Sedlak, J. & Lindsay, R. H. (1968). Estimation of total protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent. Analytical Biochemistry, 25, 192-205.

Seino, K.K., Foreman, J.H., Greene, S.A., Goetz, T.E. & Benson, G.J., (2003). Effects of topical perineural capsaicin in a reversible model of equine foot lameness. Journal of Veterinary Internal Medicine, 17, 563-566.

Silva, D. J. & Queiroz, A. C., (2002). Food analysis: chemical and biological methods. Viçosa: Universidade Federal de Viçosa.

Takano, F., Yamaguchi, M., Takada, S., Shoda, S., Yahagi, N., Takahashi, T. & Ohta, T., (2007). Capsicum ethanol extracts and capsaicin enhance interleukin-2 and interferon-gamma production in cultured murine Peyer's patch cells ex vivo. Life Science, 89, 1553-1563.

Van Soest, P.J., Robertson, J.B. & Lewis, B.A., (1991). Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74, 3583-3597.

Van de Wall, E. H., Gram, D.X., Strubbe, J.H., Scheurink, A.J. & Koolhaas, J.M., (2005.) Ablation of capsaicin-sensitive afferent nerves affects insulin response during an intravenous glucose tolerance test. Life Science, 77, 1283-1292.

Van de Wall, E. H, Wielinga, P.Y., Strubbe, J.H. & Van Dijk, G., (2006). Neonatal capsaicin causes compensatory adjustments to energy homeostasis in rats. Physiology and Behavior, 89, 115-121.

Vinã, J., Borras, C. & Gomez-Cabrera, M.C., (2018). A free radical theory of frailty. Free Radical Biology and Medicine, 124, 358-363.

Zafra, M. A., F. Molina & A. Puerto. (2003). Effects of perivagal administration of capsaicin on post-surgical food intake. Autonomic Neuroscience, 107, 37–44.

Zhao, J., Wei, F., Xu, W. & Han, X., (2020). Enhanced antibacterial performance of gelatin/chitosan film containing capsaicin loaded MOFs for food packaging. Applied Surface Science, 510, 14518. https://doi.org/10.1016/j.apsusc.2020.145418

Zhuang, Y., Chen, L., Sun, L. & Cao, J., (2012). Bioactive characteristics and antioxidant activities of nine peppers. Journal of Functional Foods, 4, 331-338.

https://doi.org/10.1016/j.jff.2012.01.001

Downloads

Published

24/11/2020

How to Cite

CUNHA, M. G. .; ALBA, D. F. .; LEAL, K. W. .; MARCON, H.; SOUZA, C. F. .; BALDISSERA, M. D. .; PAGLIA, E. B. .; KEMPKA, A. P. .; VEDOVATTO, M.; ZOTTI, C. A.; SILVA, A. S. D. . Inclusion of pepper extract containing capsaicin in the diet of ewes in the mid-lactation period: effects on health, milk production, and quality. Research, Society and Development, [S. l.], v. 9, n. 11, p. e46791110020, 2020. DOI: 10.33448/rsd-v9i11.10020. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/10020. Acesso em: 3 dec. 2024.

Issue

Section

Agrarian and Biological Sciences