High-moisture corn grain silage inoculated with Propionibacterium acidipropionici and Lactobacillus plantarum in different storage times
DOI:
https://doi.org/10.33448/rsd-v10i1.10371Keywords:
Enterobacteria; Microbial additive; pH; Stability.Abstract
The aim of this study was to evaluate the chemical composition, growth of microorganisms, and the aerobic stability of high-moisture corn grain silage inoculated with Lactobacillus plantarum + Propionibacterium acidipropionici. The experimental design was completely randomized in a 2 x 5 factorial arrangement with four replications (50 experimental units) and the treatments were: use or not of microbial inoculant (2 x 105 colony forming unit (CFU) g-1 Lactobacillus plantarum + Propionibacterium acidipropionici) and the storage length for 0, 1, 3, 7, and 14 d. Aerobic stability of silage was evaluated at 28 and 56 d of storage length. The lactic acid bacteria population was influenced by storage length and the greatest values were estimated at 8 d of storage length. Regardless of inoculant application, no enterobacteria were present from 3 d post-ensiling. With respect to mold growth, an interaction between inoculant and storage length was observed wherein molds were most abundant after 3 d of storage in silage that received inoculant. However, at 14 d of storage the use of bacterial inoculant reduced the occurrence of molds. pH values obtained after 3 d of ensiling were less than 4.0 for all the treatments. The high-moisture corn grain silage possessed good fermentative quality. Further, adequate pH values were achieved from the third day of ensilage and were not influenced by the presence of bacterial inoculant.
References
Arriola, K. G., Kim, S. C. & Adesogan, A. T. (2011). Effect of applying inoculants with heterolactic or homolactic and heterolactic bacteria on the fermentation and quality of corn silage. Journal of Dairy Science, 94(3),1511-1516.
AOAC- Association of Official Analytical Chemists. 1990. Official methods of analysis. 15th ed. AOAC International, Arlington, VA.
Avila, C. L. S. & Carvalho, B. F. (2020). Silage fermentation - updates focusing on the performance of micro-organisms. Journal of Applied Microbiology, 128(4), 966-984.
Carvalho, I. R., Souza, V. Q., Follmann, D. N., Nardino, M., Pelegrin, A. J., Ferrari, M., Konflanz, V. A., Lazzari, R. & Uczay, J. (2016). Silage production and bromatological constitution effects of corn hybrids in different environments. Revista de Ciências Agrárias, 39(2), 242-250.
Cherney, J. H. & Cherney, D. J. R. (2003). Assessing silage quality. In: Silage Science and Technology (pp. 141-198). Wisconsin.
Da Silva, T. C., Smith, M.L., Barnard, A. M. & Kung Jr., L. (2015). The effect of a chemical additive on the fermentation and aerobic stability of high-moisture corn. Journal of Dairy Science, 98(12), 8904–8912.
Driehuis, F., Wilkinson, J. M., Jiang, Y., Ogunade, I. & Adesogan, A. T. (2018). Silage review: animal and human health risks from silage. Journal of Dairy Science, 101(5), 4093–4110.
Ferraretto, L. F., Crump, P. M. & Shaver, R. D. (2013). Effect of cereal grain type and corn grain harvesting and processing methods on intake, digestion and milk production by dairy cows through a meta-analysis. Journal of Dairy Science, 96(1), 533–550.
Filya, I., Sucu, E. & Karabulut, A. (2004). The effect of Propionibacterium acidipropionici, with or without Lactobacillus plantarum, on the fermentation and aerobic stability of wheat, sorghum and maize silages. Journal of Applied Microbiology, 97, 818–826.
Krüger, A. M., Lima, P. M. T., Abdalla Filho, A. L., Moro, J. G., Carvalho, I. Q., Abdalla, A. L. & Jobim, C. C. (2020). Dry matter concentration and corn silage density: Effects on forage quality. Tropical Grasslands-Forrajes Tropicales, 8(1), 20–27.
Kung, Jr., L., Shaver, R. D., Grant, R. J. & Schmidt, R. J. (2018). Silage review: Interpretation of chemical, microbial, and organoleptic components of silages. Journal of Dairy Science, 101(5), 4020-4033.
Kung Jr., L., Sheperd, A. C., Smagala, A. M., Endres, K. M., Bessett, C. A., Ranjit, N. K. & Glancey, J. L. (1998). The effect of preservatives based on propionic acid on the fermentation and aerobic stability of corn silage and a total mixed ration. Journal of Dairy Science, 81(5), 1322–1330.
Kung, L. Jr., Schmidt, R. J., Ebling, T. E., & Hu, W. (2007). The effect of Lactobacillus buchneri 40788 on the fermentation and aerobic stability of ground and whole high moisture corn. Journal of Dairy Science, 90(5), 2309-2314.
Kung Jr., L., Stokes, M. R. & Lin, C. J. (1993). Silage Additives, pp. 305-360. In: Silage Science and Technology. Buxton, D. R., Muck, R. E.; Harrison, J. H., ed. American
Society of Agronomy, Madison.
Kung, L. Jr., Windle, M. C. & Walker N. (2014). The effect of an exogenous protease on the fermentation and nutritive value of high-moisture corn. Journal of Dairy Science, 97(3), 1707–1712.
Muck, R. E. 2010. Silage microbiology and its control through additives. Revista Brasileira de Zootecnia, 9, 183-191.
Morais, G., Daniel, J. L. P., Kleinshmitt, C., Carvalho, P. A., Fernandes, J. & Nussio, L. G. (2017). Additives for Grain Silages: A Review. Slovak Journal of Animal Science, 50(1), 42–54.
O´Kiely, P. O., Clancy, M. & Doyle, E. M. (2001). Aerobic stability of grass silage mixed with a range of concentrate feedstuffs at feed-out (pp. 794-495). In: International Grassland Congress, São Pedro.
Pahlow, G., Muck, R. E., Driehuis, F., Oude Elferink, S. J. W. H. & Spoelstra, S. F. (2003). Microbiology of ensiling (pp. 31-39). In: Silage Science and Technology. Buxton, D. R., Muck, R. E., Harrison, J. H. ed. American Society Agronomy, Madison.
Parizzi, L. P., Grassi, M. C. B., Llerena, L. A., Carazzolle, M. F., Queiroz, V. L., Lunardi, I., Zeidler, A. F., Teixeira, P. J. P. L., Mieczkowski, P., Rincones, J. & Pereira, G. A. G. (2012). The genome sequence of Propionibacterium acidipropionici provides insights into its biotechnological and industrial potential. BMC Genomics, 13, 562.
Restelatto, R., Novinski, C. O., Pereira, L. M., Silva, E. P. A., Volpi, D., Zopollatto, M., Schmidt, P. & Faciola, A. P. (2019). Chemical composition, fermentative losses, and microbial counts of total mixed ration silages inoculated with different Lactobacillus species. Journal of Animal Science, 97(4), 1634-1644.
Rowghani, E., Zamiri, M. J., Khorvash, M. & Abdollahipanah, A. (2008). The effects of Lactobacillus plantarum and Propionibacterium acidipropionici on corn silage fermentation, ruminal degradability and nutrient digestibility in sheep. Iranian Journal of Veterinary Research, 9(4), 308-305.
Saylor, B. A., Casale, F., Sultana, H. & Ferraretto, L. F. (2020). Effect of microbial inoculation and particle size on fermentation profile, aerobic stability, and ruminal in situ starch degradation of high-moisture corn ensiled for a short period. Journal of Dairy Science, 103(1), 379–395.
Silva, N., Junqueira, V. C. A. & Silveira, N. F. A. (1997). Manual de métodos de análise microbiológica de alimentos. São Paulo: Varela.
Silva, N. C., Nascimento, C. F., Campos, V. M. A., Alves, M. A. P., Resende, F. D., Daniel, J. L. P. & Siqueira G. R. (2019). Influence of storage length and inoculation with Lactobacillus buchneri on the fermentation, aerobic stability, and ruminal degradability of high-moisture corn and rehydrated corn grain silage. Animal Feed Science and Technology, 251, 124–133.
Silva, N. C., Nascimento, C. F., Nascimento, F. A., Resende, F. D., Daniel, J. L. P. & Siqueira, G. R. (2018). Fermentation and aerobic stability of rehydrated corn grain silage treated with different doses of Lactobacillus buchneri or a combination of Lactobacillus plantarum and Pediococcus acidilactici. Journal of Dairy Science, 101(5), 4158–4167.
Souza, W. L., Cidrini, I.A., Neiva Júnior, A. P., Silva, M. D., Gervásio, J. R. S., Abreu, M. J. I. & Nascimento, D.C. (2020). Effect of rehydration with whey and inoculation with Lactobacillus plantarum and Propionibacterium acidipropionici on the chemical composition, microbiological dynamics, and fermentative losses of corn grain silage. Semina: Ciências Agrárias, 41(6), 3351-3364.
Van Soest, P. J., Robertson, J. B. & Lewis, B. A. (1991). Methods for dietary fiber, neutral detergent fiber and non starch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74(10), 3583-3597.
Vila-Donat, P., Marín, S., Sanchis, V. & Ramos, A. J. (2018). A review of the mycotoxin adsorbing agents, with an emphasis on their multi-binding capacity, for animal feed decontamination. Food and Chemical Toxicology, 114, 249–259.
Woolford, M. K. 1990. The detrimental effects of air on silage. Journal of Applied Bacteriology, 68(2), 101–116.
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Copyright (c) 2021 Rafael Frank; Marcela Abbado Neres; Tatiane Fernandes; Rodrigo André Schöne; André Sanches de Avila; Edinan Hagdon Cirilo; Nilton Rohloff Junior; Cristine Kaufmann; Clauber Polese; Felipe Potenza Campos; Guilherme Luis Silva Tesser; Tânia Luiza Kohler; Cleison Souza; Nathanael Cesar Costa Santos; Ana Paula Guimarães Cruz Costa; Ricardo Vianna Nunes
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