Influence of storage temperature on the population of microorganisms in raw sheep milk and its physical-chemical profile

Authors

DOI:

https://doi.org/10.33448/rsd-v9i12.10796

Keywords:

Quality; Dairy products; Composition; Refrigeration; Shelf life.

Abstract

The aim of the present study was to evaluate the physical-chemical profile of raw sheep milk and the influence of storage temperature on the population of microorganisms. Sheep milk samples were collected from the milk cans (500 mL/sample) and were evaluated the physical-chemical composition: fat, protein, lactose, ash content, total dry matter (TDM), cryoscopy index (°H), titratable acidity, and somatic cell count (SCC). After the samples were stored at 4°C and 9°C (72 h) and the titratable acidity, the population of the deteriorating microorganisms and quality indicators were determined. PetrifilmTM (3M do Brasil Ltda) were used for the mesophilic aerobic count (37°C/48 h), total coliforms (37°C/24 h), Escherichia coli (37°C/48 h), and enterobacteria (37°C/24 h). For psychrotrophic bacteria, was used Plate Count Agar (7ºC/10 days) and for Pseudomonas spp., CFC-supplemented Pseudomonas agar base (25ºC/48 h). Average values for raw milk were 6.28% fat, 83.46% moisture, 16.52% TDM, 0.92% ash, 5.30% protein, 4.43% lactose, a cryoscopy index of -0.575°H, and SCC of 3.38 x 106 cells/mL. Milk acidity values were higher (0.24 g lactic acid/100 ml) with 72 hours of storage. The higher the temperature and/or storage time, the higher the counts of psychrotrophic, Pseudomonas spp., and enterobacteria, for mesophilic aerobic only the storage time. Refrigeration at 4ºC and a reduction of storage time are important to control the microorganism’s population that compromise the quality, shelf life, and food safety of sheep milk and dairy products.

References

Albenzio, M., Figliola, L., Caroprese, M., Marino, R., Sevi, A., & Santillo, A. (2019). Somatic cell count in sheep milk. Small Ruminant Research, 176, 24-30. https://doi.org/10.1016/j.smallrumres.2019.05.013

AOAC - Association of Official Analytical Chemists (2005). Official Methods of Analysis of AOAC International, 18th edn. Gaithersburg: MD.

Balthazar, C. F., Pimentel, T. C., Ferrão, L. L., Almada, C. N., Santillo, A., Albenzio, M., Mollakhalili, N., Nascimento, J. S., Silva, M. C., Freiras, M. Q., Santana, A. S., Granato, D., & Cruz, A. G. (2017). Sheep milk: Physicochemical characteristics and relevance for functional food development. Comprehensive Reviews in Food Science and Food, 16 (2), 247-262. https://doi.org/10.1111/1541-4337.12250

Barłowska, J., Szwajkowska, M., Litwinczuk, Z., & Krol, J. (2011). Nutritional value and technological suitability of milk from various animal species used for dairy production. Comprehensive Reviews in Food Science and Food Safety, 10, 291-302. https://doi.org/10.1111/j.1541-4337.2011.00163.x

Ferro, M. M., Tedeschi, L. O., & Atzori, A. S. (2017). The comparison of the lactation and milk yield and composition of selected breeds of sheep and goats. Translational Animal Science, 1 (4), 498–506. https://doi.org/10.2527/tas2017.0056

Frank, J. F., & Yousef, A. E. (2004). Tests for groups of microorganisms. In: Wehr, M. W., Frank, J. F. Standard methods for the examination of dairy products (ed.). New York: APHA, 227-247.

Franco, B. D. G. M., & Landgraf, M. (2008). Microbiologia dos Alimentos (2 ed.). São Paulo: Atheneu.

ISO - International Organization for Standardization (2009). Milk and milk products: Method for the enumeration of Pseudomonas spp., norm 11059. Geneva, Switzerland (IDF/RM 225).

McAuley, C. M., Singh, T. K., Haro-Maza, J. F., Williams, R., & Buckow, R. (2016). Microbiological and physicochemical stability of raw, pasteurised or pulsed electric field-treated milk. Innovative Food Science and Emerging Technologies, 38, 365-373. https://doi.org/10.1016/j.ifset.2016.09.030

Merlin Junior, I. A., Santos, J. S., Costa, L. G., Costa, R. G., Ludovico, A., Rego, F. C. A., & Santana, E. H. W. (2015). Sheep milk: physical-chemical characteristics and microbiological quality. Archivos Latinoamericanos de Nutrición, 65 (3), 193-198.

Mohapatraa, A., Shindeb, A. K., & Singh, R. (2019). Sheep milk: A pertinent functional food. Small Ruminant Research, 181, 6-11. https://doi.org/10.1016/j.smallrumres.2019.10.002

Molineri, A. I., Signorini, M. L., Cuatrín, A. L., Canavesio, V. R., Neder, V. E., Russi, N. B., Bonazza, J. C. & Calvinho, L. F. (2012) Association between milking practices and psychrotrophic bacterial counts in bulk tank milk. Revista Argentina de Microbiologia, 44, 187-194.

Panayotov, D., Naydenova, K., Mihaylova, N., & Iliev, T. (2018). Physico-chemical and technological characteristics of Lacaune ewe’s milk. Bulgarian Journal of Agricultural Science, 24 (sup. 1), 101-108.

Rafiq, S., Huma, N., Pasha, I., Sameen, A., Mukhtar, O. & Khan, M. I. (2016). Chemical Composition, Nitrogen Fractions and Amino Acids Profile of Milk from Different Animal Species. Asian-Australas. Journal of Animal Science, 29 (7), 1022-1028. https://doi.org/10.5713/ajas.15.0452

Santana, E. H. W., Beloti, V., Barros, A. F., Moraes, L. B., Gusmão, V. V., & Pereira, M. S. (2001). Milk contamination in different points of the process: Aerobc mesophilics and psychrotrophic aerobic microorganisms. Semina: Ciências Agrárias, 22 (2), 145-154. http://dx.doi.org/10.5433/1679-0359.2001v22n2p145

Silva, N., Junqueira, V. C. A., & Silveira, N. F. A. (1997). Coleta / Transporte / Estocagem / Preparação de amostras para análise. In: Silva, N. Manual de métodos de análise microbiológica de alimentos. São Paulo: Livraria Varela, 8 -20.

Sobrino, L. J., Colado, J. M. P., Sigler, A. I. G., Marín, A. L. M., Sánchez, N. N., & Romero, J. (2018). Composition and colour indices of sheep’s bulktank milk are influenced by production practices. Italian Journal of Animal Science, 17 (2), 477–488. https://doi.org/10.1080/1828051X.2017.1383860

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Published

24/12/2020

How to Cite

BRUZAROSKI, S. R.; SOUZA, R. P. de; PASQUIM, P. da S.; FAGNANI, R.; SANTANA, E. H. W. de. Influence of storage temperature on the population of microorganisms in raw sheep milk and its physical-chemical profile . Research, Society and Development, [S. l.], v. 9, n. 12, p. e27691210796, 2020. DOI: 10.33448/rsd-v9i12.10796. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/10796. Acesso em: 22 nov. 2024.

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Section

Agrarian and Biological Sciences