Antimicrobial action of the hydroethanolic extract of Spondias mombin L. against oral bacteria of the genus Streptococcus

Authors

DOI:

https://doi.org/10.33448/rsd-v10i1.11539

Keywords:

Streptococcus; Phytotherapy; Chlorhexidine; Bactericidal agent.

Abstract

Objetivo: O objetivo do presente estudo foi avaliar in vitro a atividade antimicrobiana do extrato hidroetanólico da folha de Spondias mombin (S. mombin L.) sobre bactérias do gênero Streptococcus: S. mutans, S. mitis, S. oralis, S. sanguinis e S. salivarius, comparando-a à atividade antimicrobiana da solução de digluconato de clorexidina 0,12% sobre essas mesmas bactérias. Metodologia: Foram realizados os testes in vitro de Determinação da Concentração Inibitória Mínima, Determinação da Concentração Inibitória Mínima de Aderência e Análise da Cinética Bactericida. Resultados: Para o Streptococcus mutans, o grupo controle foi estatisticamente superior (p<0,05) ao extrato até a concentração 31,25mg/ml, não ocorrendo diferença estatística nas demais concentrações. Para o Streptococcus oralis, o extrato foi estatisticamente superior (p<0,05) ao grupo controle em sua forma bruta (500mg/ml), o grupo controle teve significância estatística (p<0,05) sobre o extrato apenas nas concentrações 62,5mg/ml, 31,25mg/ml, 1,95mg/ml e 0,97mg/ml. Para o Streptococcus sanguinis, foi constatado que o extrato foi estatisticamente superior (p<0,05) ao grupo controle nas duas primeiras concentrações 500mg/ml e 250mg/ml, ao passo que o grupo controle teve significância estatística (p<0,05) sobre o extrato apenas nas duas últimas concentrações: 1,95mg/ml e 0,97mg/ml. O grupo controle foi estatisticamente superior (p<0,05) ao extrato em todas as concentrações no Streptococcus mitis, exceto para a concentração 31,25mg/ml. Para a Streptococcus salivarius, o extrato foi estatisticamente superior (p<0,05) ao grupo controle na maioria das concentrações testadas, com exceção apenas para 15,65mg/ml e 1,95mg/ml, que não foram observados diferenças estatisticamente significativas entre o extrato e o grupo controle. Conclusão: o extrato hidroetanólico de Spondias mombin L., em diferentes concentrações, apresenta atividade antimicrobiana semelhante ao digluconato de clorexidina a 0,12% frente a bactérias bucais do gênero Streptococcus.

References

Aleluia, C. M., et al. (2015). Medicines In Dentist Herbal. Rev. Odontol. Univ. São Paulo, 27 (2), 126-134.

Alencar, L. C. B., et al. (2015). Modulatory effect of the extract of medicinal plants of the genus Spondias on the resistance of Staphylococcus aureus strains to Erythromycin. Journal of Basic and Applied Pharmaceutical Sciences, 36(1).

Asuquo, O. R., et al. (2012). Hitomorphological study of the anti-fertility effect of Spondias mombin L. in adult male rats. Journal of pharmacyand biological sciences, 3, 29-34.

Bauer, A. W., Kirby, W. M., Sherris, J. C., & Turck, M. (1966). Antibiotic susceptibility testing by a standardized single disk method. American journal of clinical pathology, 45(4), 493.

Bergenholtz, G. (2016). Assessment of treatment failure in endodontic therapy, J. Oral.Rehabil., 753-758.

Bueno-Silva, B. et al. (2013). Effect of neovestitol – vestitol containing Brazilian red propolis on accumulation of biofilm in vitro and development of dental caries in vivo. Biofouling, 29, 1233-1242.

Cabral, B., Siqueira, E., Bitencourt, M. A., Lima, M. C., Lima, A. K., Ortmann, C. F., & Reginatto, F. H. (2016). Phytochemical study and anti-inflammatory and antioxidant potential of Spondias mombin leaves. Revista Brasileira de Farmacognosia, 26 (3), 304-311.

Chiniforush, N., Pourhajibagher, M., Shahabi S., Kosarieh E. & Bahador, A. (2016). Can Antimicrobial Photodynamic Therapy (aPDT) Enhance the Endodontic Treatment? J. Lasers. Med. Sci., 76-85.

Flemming, Hans-Curt & Wingender, Jost (2010). The biofilm matrix. Nature reviews microbiology, 8(9), 623-633.

Gebara, E. C. E., Zardetto, C. G. D. C., & Mayer, M. P. A. (1996).In vitro study of the antimicrobial action of natural substances on S. mutans and S.sobrinus. Rev Odontol Univ. 10 (4), 251- 256.

Gülçin, İ., Huyut, Z., Elmastaş, M., & Aboul-Enein, H. Y. (2010). Radical scavenging and antioxidant activity of tannic acid. Arabian Journal of Chemistry, 3(1), 43-53.

Gunsolley, J. C. (2010). Clinical efficacy of antimicrobial mouthrinses. Journal Dent., 38, S6-S10.

Hajifattahi, F., Maravej, S. E., Taheri, M., Arash, M., & Kamalinejad, M. (2016). Antibacterial effect of hydroalcoholic extract of Punica granatum Linn. Petal on common oral microorganisms. Inter. J. Biomat, 1-6.

Jesus, R. P. F. S., et al. (2010). Antibacterial and nonstick action of pithecellobium Cochliocarpum (gomez) macbr on oral microorganisms. Odontol. Clín.-Cient. 9 (4), 331-335.

Jongsma, M. A., Van de Lagemaat, M., Busscher, H. J., Geertsema-Doornbusch, G. I., & Atema-Smit, J., Van Der Mei, H. C., & Ren, Y.(2015). Synergy of brushing mode and antibacterial use on in vivo biofilm formation. Journal of dentistry, 43(12), 1580-1586.

Khémiri,I. et al. (2019).The A ntimicrobial and Wound Healing Potential of Opuntia ficus indica L. inermis Extracted Oil from Tunisia. Evid Based Complement Alternat Med. 10.1155/2019/9148782

Marsh, P. D. (2010). Controlling the oral biofilm with antimicrobials. Journal Dent., 38, S11-S15.

Matos, L. M. R. et al. (2015). Effect of Antiseptic with and without alcohol on the oral microbiota. R. Interd, 8, (4), 174-180.

Medeiros, P. M., Ladio, A. H., Santos, A. M. M., & de Albuquerque, U. P. (2013). Does the selection of medicinal plants by Brazilian local populations suffer taxonomic influence? Journal of ethnopharmacology, 146(3), 842-852.

Natarajan, D., Britto, S. J., Srinivasan, K., Nagamurugan, N., Mohanasundari, C., & Perumal, G. (2005). Anti-bacterial activity of Euphorbia fusiformis—A rare medicinal herb. Journal of ethnopharmacology, 102 (1), 123-126.

Palombo E.A. (2011). Traditional Medicinal Plant Extracts and Natural Products with Activity against Oral Bacteria: Potential Application in the Prevention and Treatment of Oral Diseases. Evid Based Complement Alternat Med. doi.org/10.1093/ecam/nep067.

Pan, P. C. et al. (2010). In-vitro evidence for efficacy of antimicrobial mouthrinses. Journal Dent., 38, S16-S20.

Pereira A. S. et al. (2018). Scientific research methodology. Ed. UAB/NTE/UFSM. https://repositorio.ufsm.br/bitstream/ handle/1/15824/Lic_Computacao_Metodologia-Pesquisa-Cientifica.pdf?sequence=1.

Peyret, M., Carret, G., Carre, C., Fardel, G., & Flandrois, J. P. (1990). Mathematical study of the sensitivity curves of Escherichia coli exposed to polymyxins. Pathol Biol, 38,441-445.

Pourhajibagher, M., & A. Bahador, A. (2015). Is antimicrobial agent can considered as effective weapons against endodontic infections by Enterococcus faecalis? Der Pharma Chemica, 196–200.

Pyysalo, Mikko J. et al. (2019). Increased brushing frequency is associated with reduced gingival bag bacterial diversity in patients with intracranial aneurysms. Peer Journal, 7,6316.

Quintas, V., Prada-Lopez, I., Donos, N., Suarez-Quintanilla, D., & Tomas, I. (2015). In situ neutralisation of the antibacterial effect of 0.2% Chlorhexidine on salivary microbiota: Quantification of substantivity. Archives of oral biology, 60(8), 1109-1116.

Sadaka F., Nguimjeu C., Vroman I., Tighzert L. & Couvercelle J. P. (2014). Review on antimicrobial packaging containing essential oils and their active biomolecules. Innov. Food Sci. Emerg. Technol: Brachais CH.

Schlafer, S., Raarup, M. K., Meyer, R. L., Sutherland, D. S., Dige, I., Nyengaard, J. R., et al.(2011). pH Landscapes in a novel five-species model of early dental biofilm. PLoS One, 6 (9).

Shakerian, M., Yaghoti, M. M., & Doostaki, S. (2018). In Vitro Effect of Hydroalcoholic Extract of Aloe Vera and 0.2% Chlorhexidine Mouthwash on Streptococcus Sanguinis, Streptococcus Salivarius and Streptococcus Mutans. Journal of Dental School, Shahid Beheshti University of Medical Sciences, 36 (1), 18-22.

Silva, V. A. et al. (2013). Antimicrobial efficacy of the extract of Croton sonderianus Müll. on bacteria that cause dental caries. Revista de Odontologia da UNESP, 40 (2), 69-72.

Smania Jr, A., Monache, F. D., Smania, E. D. F. A., & Cuneo, R. S. (1999). Antibacterial activity of steroidal compounds isolated from Ganoderma applanatum (Pers.) Pat. (Aphyllophoromycetideae) fruit body. International Journal of medicinal mushrooms, 1(4).

Stewart, P. S., Rayner, J., Roe, F., & Rees W. M, (2001). Biofilm penetration and disinfection efficacy of alkaline hypochlorite and chlorosulfamates. Journal Appl Microbiol; 91:525-32.

Swango, P. A. (2012). Regular use of antimicrobial mouthrinses can effectively augment the benefits of oral prophylaxis and oral hygiene instructions at 6-month recall intervals in reducing the occurrence of dental plaque and gingivitis. Journal Evid Based Dent Pract., 12 (2), 87-89.

Thadhani, V. M., Choudhary, M. I., Khan, S., & Karunaratne, V. (2012). Antimicrobial and toxicological activities of some depsides and depsidones. Journal of the National Science Foundation of Sri Lanka, 40(1), 43-48.

Torres, C. R. G., Kubo, C. H., Anido, A., & Rodrigues, J. R. (2000). Antimicrobial agents and your potential of use in odontology. PGR: Pós-Grad Rev Fac Odontol São José dos Campos. 3, 43-52.

Van der Weijden, G. A., & Hioe, K. P. K. (2005). A systematic review of the effectiveness of mechanical plaque removal performed in adults with gingivitis using a manual toothbrush. Jornal de Periodontologia clínica, 32, 214-228.

Yao, Y., Yang, X., Shi, Z., & Ren, G. (2014). Anti‐Inflammatory Activity of Saponins from Quinoa (Chenopodium quinoa Willd.) Seeds in Lipopolysaccharide‐Stimulated RAW Macrophages Cells. Journal of food science, 79(5).

Zheng, L. W., Hua, H., & Cheung, L. K. (2011). Traditional Chinese medicine and oral diseases: today and tomorrow. Oral Dis., 17, (1), 7-12.

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Published

19/01/2021

How to Cite

CORDEIRO, T. O. .; MENDONÇA, A. K. P. de; GOMES, M. S. .; LIMA, E. L. F. de .; COSTA, M. R. M. .; LIMA, K. C. de .; LINS , R. D. A. U. . Antimicrobial action of the hydroethanolic extract of Spondias mombin L. against oral bacteria of the genus Streptococcus. Research, Society and Development, [S. l.], v. 10, n. 1, p. e39310111539, 2021. DOI: 10.33448/rsd-v10i1.11539. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/11539. Acesso em: 28 feb. 2021.

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Health Sciences