Effect of Bacillus aryabhattai on the initial establishment of pre-sprouted seedlings of sugarcane varieties
Keywords:seedlings; Saccharum officinarum L.; biological active ingredient; inoculation
This study proposes to examine the potential use of Bacillus aryabhattai in pre-sprouted seedlings of sugarcane inoculated with the microorganism when subjected to different regimes of water supply after transplanting. The experiment was laid out in a randomized-block design with a complete 3 × 2 × 5 factorial arrangement, in five replicates. The factors were represented by pre-sprouted seedlings of three sugarcane varieties (IAC 911099, RB 855156 and CTC 20), two seedling types (inoculated and not inoculated with B. aryabhattai) and five frequencies of water supply, which provided the ability of return to 100% soil field capacity, at every 0, 10, 20, 30 and 40 days. Plant mortality, plant height, number of leaves, stalk diameter and number of tillers were evaluated throughout the experimental period. At the end of the study, the shoot dry matter (SDM) and root dry matter (RDM) contents of the plants were measured. The number of leaves on the plant was affected only in isolation, according to the cultivar, without effects of the other studied factors. There was a double interaction effect between the factors of variety and inoculation (V*I) for the variables of SDM, stalk diameter and height; and between frequency and inoculation (F*I) for stalk diameter. There was a triple interaction effect between variety, inoculation and frequency (V*I*F) for the RDM variable. Thus, the use of B. aryabhattai as an inoculant in pre-sprouted seedlings of sugarcane at the time of seedling formation can improve plant development after transplanting depending on the cultivar used, especially in IAC 911099 and RB 855156.
Ahmad, M., Adil, Z., Hussain, A., Mumtaz, M. Z., Nafees, M., Ahmad, I., & Jamil, M. (2019). Potential of phosphate solubilizing Bacillus strains for improving growth and nutrient uptake in mungbean and maize crops. Pakistan Journal of Agricultural Sciences, 56(2).
Araújo, F. F. D., & Hungria, M. (1999). Nodulação e rendimento de soja co-infectada com Bacillus subtilis e Bradyrhizobium japonicum/Bradyrhizobium elkanii. Pesquisa agropecuária brasileira, 34(9), 1633-1643.
Araujo, F. F., Henning, A. A., & Hungria, M. (2005). Phytohormones and antibiotics produced by Bacillus subtilis and their effects on seed pathogenic fungi and on soybean root development. World Journal of Microbiology and Biotechnology, 21(8-9), 1639-1645.
Cassán, F., Vanderleyden, J., & Spaepen, S. (2014). Physiological and agronomical aspects of phytohormone production by model plant-growth-promoting rhizobacteria (PGPR) belonging to the genus Azospirillum. Journal of Plant Growth Regulation, 33(2), 440-459.
Cavallet, L. E., Pessoa, A. C. D. S., Helmich, J. J., Helmich, P. R., & Ost, C. F. (2000). Produtividade do milho em resposta à aplicação de nitrogênio e inoculação das sementes com Azospirillum spp. Revista Brasileira de Engenharia Agrícola e Ambiental, 4(1), 129-132.
Chaves, V. A., Santos, S. G. D., Schultz, N., Pereira, W., Sousa, J. S., Monteiro, R. C., & Reis, V. M. (2015). Desenvolvimento inicial de duas variedades de cana-de-açúcar inoculadas com bactérias diazotróficas. Revista Brasileira de Ciencia do solo, 39(6), 1595-1602.
Damam, M., Kaloori, K., Gaddam, B., & Kausar, R. (2016). Plant growth promoting substances (phytohormones) produced by rhizobacterial strains isolated from the rhizosphere of medicinal plants. International Journal of Pharmaceutical Sciences Review and Research, 37(1), 130-136.
Dimkpa, C., Weinand, T., & Asch, F. (2009). Plant–rhizobacteria interactions alleviate abiotic stress conditions.Plant, cell & environment, 32(12), 1682-1694.
Gazola, T., Cipola Filho, M. L., & Júnior, N. C. F. (2017). Avaliação de mudas pré-brotadas de cana-de-açúcar provenientes de substratos submetidos a adubação química e orgânica. Científica, 45(3), 300-306.
Kavamura, V. N., Santos, S. N., da Silva, J. L., Parma, M. M., Ávila, L. A., Visconti, A., ... & de Melo, I. S. (2013). Screening of Brazilian cacti rhizobacteria for plant growth promotion under drought. Microbiological research, 168(4), 183-191.
Lee, Y. H., Jang, S. J., Han, J. H., Bae, J. S., Shin, H., Park, H. J. & Hong, J. K. (2018). Enhanced Tolerance of Chinese Cabbage Seedlings Mediated by Bacillus aryabhattai H26-2 and B. siamensis H30-3 against High Temperature Stress and Fungal Infections. The Plant Pathology Journal, 34(6), 555.
Lesueur, D., Deaker, R., Herrmann, L., Bräu, L., & Jansa, J. (2016). The production and potential of biofertilizers to improve crop yields. In Bioformulations: for sustainable agriculture (pp. 71-92). Springer, New Delhi.
Matoso, E. S., De Marco, E., Bellé, C., Rodrigues, T. A., & dos Anjos, S. D. (2016). Desenvolvimento inicial de mudas pré-brotadas de cana-de-açúcar inoculadas com bactérias diazotróficas. Revista da Jornada de Pós-Graduação e Pesquisa-Congrega Urcamp, 412-434.
May, A., Ramos, N., Santos, M. D. S., da Silva, E. H. F. M., & de Melo, I. S. (2019). Promoção de crescimento de mudas pré-brotadas de cana-de-açúcar inoculadas com Bacillus aryabhattai em diferentes frequências de irrigação. Embrapa Meio Ambiente-Boletim de Pesquisa e Desenvolvimento (INFOTECA-E).
Mirza, M. S., Ahmad, W., Latif, F., Haurat, J., Bally, R., Normand, P., & Malik, K. A. (2001). Isolation, partial characterization, and the effect of plant growth-promoting bacteria (PGPB) on micro-propagated sugarcane in vitro. Plant and Soil, 237(1), 47-54.
Nadeem, S. M., Ahmad, M., Zahir, Z. A., Javaid, A., & Ashraf, M. (2014). The role of mycorrhizae and plant growth promoting rhizobacteria (PGPR) in improving crop productivity under stressful environments. Biotechnology advances, 32(2), 429-448.
Oliveira, A. L. M., de Canuto, E. L., Urquiaga, S., Reis, V. M., & Baldani, J. I. (2006). Yield of micropropagated sugarcane varieties in different soil types following inoculation with diazotrophic bacteria. Plant and Soil, 284(1-2), 23-32.
Oliveira, T. B. A., Selig, P. M., Barbosa, V. M., de Souza Campos, L. M., Bornia, A. C., & de Oliveira, M. W. (2012). Tecnologia e custos de produção de cana-de-açúcar: um estudo de caso em uma propriedade agrícola. Latin American Journal of Business Management, 3(1).
Orhan, E., Esitken, A., Ercisli, S., Turan, M., & Sahin, F. (2006). Effects of plant growth promoting rhizobacteria (PGPR) on yield, growth and nutrient contents in organically growing raspberry. Scientia Horticulturae, 111(1), 38-43.
Pereira A.S. et al. (2018). Metodologia da pesquisa científica. [e-book]. Santa Maria. Ed. UAB/NTE/UFSM. Disponível em: https://repositorio.ufsm.br/bitstream/handle/1/15824/ Lic_Computacao_Metodologia-Pesquisa-Cientifica.pdf?sequence=1.
Santos, M. D. S., Stancatte, R. S., Ferreira, T. C., Dorighello, D. V., Pazianotto, R. A. A., de Melo, I. S., ... & Ramos, N. P. (2017). Resistance to water deficit during the formation of sugarcane seedlings mediated by interaction with Bacillus sp. Científica, 45(4), 414-421.
Santos, R. D. & Rigobelo, E. (2016). Uso de Bacillus subtilis e Bacillus licheniformis na promoção de crescimento de mudas pré-brotadas de cana-de-açúcar. Ciência & Tecnologia: Fatec-JB, 8(especial).
Santos, S. G., Chaves, V. A., da Silva Ribeiro, F., Alves, G. C., & Reis, V. M. (2019). Rooting and growth of pre-germinated sugarcane seedlings inoculated with diazotrophic bacteria. Applied Soil Ecology, 133, 12-23.
Schisler, D. A., Slininger, P. J., Behle, R. W., & Jackson, M. A. (2004). Formulation of Bacillus spp. for biological control of plant diseases. Phytopathology, 94(11), 1267-1271.
Schultz, N., Morais, R. F. D., Silva, J. A. D., Baptista, R. B., Oliveira, R. P., Leite, J. M., ... & Boddey, R. M. (2012). Avaliação agronômica de variedades de cana-de-açúcar inoculadas com bactérias diazotróficas e adubadas com nitrogênio. Pesquisa Agropecuária Brasileira, 47(2), 261-268.
Spaepen, S., Vanderleyden, J., & Remans, R. (2007). Indole-3-acetic acid in microbial and microorganism-plant signaling.FEMS microbiology reviews, 31(4), 425-448.
UNICA, (2020). União da indústria de cana-de-açúcar. Disponível em: https://www.unicadata.com.br/historico-deproducao-emoagem.php?idMn=32&tipoHistorico =4. Acesso em: 14 agosto 2020.
How to Cite
Copyright (c) 2021 Andre May; Michelli de Souza dos Santos; Evandro Henrique Figueiredo Moura da Silva; Ronaldo da Silva Viana; Nilson Aparecido Vieira Junior; Nilza Patrícia Ramos; Itamar Soares de Melo
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.