Influence of constant lighting, melatonin and geldanamycin under mice testicle over 60 days

Authors

DOI:

https://doi.org/10.33448/rsd-v10i2.12731

Keywords:

Testis; Melatonin; Constant illumination; Geldanamycin; Leydig.

Abstract

Melatonin (N-acetyl-5-methoxytryptamine) is a hormone secreted by the pineal gland. In men, melatonin affects reproductive regulation in three main ways. First, it regulates the secretion of GnRH and LH. Second, it regulates testosterone synthesis and testicular maturation. Third, as a potent deputare of free radical that is both lipophilic and hydrophilic. The mammalian testis is an organ susceptible to environmental and therapeutic toxic agents that compromise spermatogenesis, the present study aimed to analyse the influence of constant illumination, melatonin and geldanamycin under the testis of rats treated for 60 days. Males were randomly divided into 4 groups, each consisting of 5 animals, which are: Group I - control rats (untreated); Group II - rats subjected to constant illumination for 60 days; Group III - rats subjected to constant illumination for 60 days and treated with melatonin for 60 days; Group IV - rats subjected to constant illumination for 60 days, treated with melatonin and geldanamycin for 60 days. Exposure to constant illumination affects the development of germinal epithelium, as well as decreased weight of the testicles, Leydig, Sertoli, and testosterone cells. Melatonin and geldanamycin treatment have a protective effect against constant illumination, reducing testicular damage when taken together.

References

Abercrombie, M. (1946). Estimation of nuclear population from microtome sections. The anatomical record, 94(2): 239-247.

Amann, R. P., & Almquist, J. O. (1962). Reproductive Capacity of Dairy Bulls. VIII. Direct and Indirect Measurement of Testicular Sperm Production1, 2, 3. Journal of Dairy Science, 45(6), 774-781.

Bahrami, N. et al. (2018). Evaluating the protective effects of melatonin on di (2-ethylhexyl) phthalate-induced testicular injury in adult mice. Biomedicine & Pharmacotherapy, v. 108, p. 515-523.

Brainard, G. C., et al. (1983). The suppression of pineal melatonin content and N-acetyltransferase activity by different light irradiances in the Syrian hamster: a dose-response relationship. Endocrinology, 113(1):293-6.

Cajochen, C., et al. (2003). Role of Melatonin in the Regulation of Human Circadian Rhythms and Sleep. Journal of Neuroendocrinology, 15(4): 432–437.

Deng, S. L., et al. (2018). Melatonin promotes sheep Leydig cell testosterone secretion in a co-culture with Sertoli cells. Theriogenology, 106: 170-177.

França, L. R., & RusselL, L. D. (1998). The testis of domestic mammals. In: Martínez-Garcia, F., & Regadera, J., (Ed.). Male reproduction: a multidisciplinary overview. España: Churchill Communications Europe España, 197- 219.

Frungieri, M., et al. (2017). Local actions of melatonin in somatic cells of the testis. International journal of molecular sciences, 18(6): 1170.

Heindel, J. J., et al. (1984). Role of the pineal in the alteration of hamster Sertoli cell responsiveness to FSH during testicular regression. J Androl, 5:211–5

Hinzpeter, A., et al. (2006). Association between Hsp90 and the ClC-2 chloride channel upregulates channel function. American Journal of Physiology-Cell Physiology, 290(1): C45-C56.

Ji, Y. L., Wang, H., Meng, C., et al. (2012). Melatonin alleviates cadmium-induced cellular stress and germ cell apoptosis in testes. J Pineal Res, 52:71–9.

Khaki, A., Fathiazad, F., Nouri, M., Khaki, A. A., Ozanci, C. C., et al (2009). The effects of ginger on spermatogenesis and sperm parameters of rat. Iran J Reprod Med., 7(1):7–12.

Kilarkaje, N. (2008). An aminoglycoside antibiotic gentamycin induces oxidative stress, reduces antioxidant reserve and impairs spermatogenesis in rats. J Toxicol Sci., 33(1):85–96

Kim, S. H., et al. (2014). Melatonin prevents gentamicin‐induced testicular toxicity and oxidative stress in rats. Andrologia, v. 46, n. 9, p. 1032-1040.

Koeberle, A, Shindou, H, Harayama, T, Yuki, K, & Shimizu, T. (2012). Polyunsaturated fatty acids are incorporated into maturating male mouse germ cells by lysophosphatidic acid acyltransferase 3. FASEB J, 26:169–80.

Legates, T. A., Fernandez, D. C., & Hattar, S. (2014). Light as a central modulator of circadian rhythms, sleep and affect. Nat Rev Neurosci. 15(7):443–54.

Li, C., & Zhou, X. (2015). Melatonin and male reproduction. Clinica Chimica Acta, 446: 175-180.

Li, C., & Zhou, X. (2015). Melatonin and male reproduction. Clinica Chimica Acta, 446, 175-180.

Lima, E. N., et al. (2013). Aspectos morfológicos, morfométricos e histoquímicos das gônadas de ratos com 30 e 60 dias de vida, nascidos de matrizes mantidas em iluminação constante. XIII JORNADA DE ENSINO, PESQUISA E EXTENSÃO – JEPEX 2013 – UFRPE, v. 52171, p. 900.

Maitra, S. K., & Ray, A. K. (2000). Role of light in the mediation of acute effects of a single afternoon melatonin injection on steroidogenic activity of testis in the rat. Journal of biosciences, 25(3): 253-256.

Moore, R. Y. (2013). The suprachiasmatic nucleus and the circadian timing system. Prog Mol Biol Transl Sci. 119:1–28.

Mylonas, C., & Kouretas, D. (1999). Lipid peroxidation and tissue damage. In Vivo,13: 295–309.

Pannocchia, M., et al. (2008). Estratégia efetiva de fixação do testículo de ratos Wistar para avaliar os parâmetros morfológicos e morfométricos do epitélio seminífero. ConScientiae Saúde, 7 (2): 227-233.

Pelletier M. R., & Vitale, L. M. (2003). Las uniones oclusivas de las barreras hematotisulares del testículo, epidídimo y conducto deferente. Sociedad Agentina de Andrologia. Dec., Buenos Aires, 12(4): 53-72.

Pereira A. S., Shitsuka D. M., Parreira F. J., & Shitsuka R. (2018). Metodologia da pesquisa científica). UAB/NTE/UFSM.

Prata Lima, M. F., et al. (2004). Effects of melatonin on the ovarian response to pinealectomy or continuous light in female rats: Similarity with polycystic ovary syndrome. Brazilian Journal of Medical and Biological Research, 37: 987–995.

Rao, M. V., & Bhatt, R. N. (2012). Protective effect of melatonin on fluoride-induced oxidative stress and testicular dysfunction in rats. Fluoride, 45:116–24. 585

Rashed, R. A., et al. (2010). Effects of two different doses of melatonin on 541 the spermatogenic cells of rat testes: a light and electron microscopic study. Egypt 542 J Histol, 33:819–35.

Reiter, R. J. (1991). Melatonin: the chemical expression of darkness. Mol Cell Endocrinol. 79(1-3):C153-8. Review.

Rocha, C. S., et al. (2015). Melatonin and male reproductive health: relevance of darkness and antioxidant properties. Current molecular medicine, 15(4): 299-31.

Semercioz, A., et al. (2018). Effects of melatonin on testicular tissue nitric oxide level and antioxidant enzyme activities in experimentally induced left varicocele. Neuro Endocrinol Lett 24:86–90.

Silva, G. A. (2018). Efeito da geldanamicina na infertilidade testicular: uma revisão de literatura. International Journal of Sex Research, 1:3

Vásquez-Ruiz, S., Maya-Barrios, J. A., Torres-Narváez, P., Vega-Martínez, B. R., Rojas-Granados, A., Escobar, C., & Angeles-Castellanos, M. (2014). A light/dark cycle in the NICU accelerates body weight gain and shortens time to discharge in preterm infants. Early Hum Dev. 90(9):535- 40.

Woodruff, D. Y., Horwitz, G., Weigel, J., & Nangia, A. K. (2010). Fertility preservation following torsion and severe ischemic injury of a solitary testis. Fertil Steril, 94, e4–5.

Wu, Y. H., & Swaab, D. F. (2005). The human pineal gland and melatonin in aging and alzheimer's disease. J Pineal Res., 38 (3), p. 145-152.

Yang, Y., et al. (2014). A review of melatonin as a suitable antioxidant against myocardial ischemia–reperfusion injury and clinical heart diseases. J Pineal Res., 57:357–66.

Downloads

Published

24/02/2021

How to Cite

COSTA, M. A. S. da .; LIRA, A. C. L. de .; GUEDES, G. H. F. .; GOMES, J. A. da S. .; CARVALHO, J. M. de .; OLIVEIRA, M. L. F. de .; SILVA, M. E. da .; GONZAGA, B. S. .; NOVIS, P. V. S. .; BRITTO, D. B. L. de A. .; TENÓRIO, B. M.; MAIA, C. S. .; MEDEIROS, J. P. de .; TENORIO, F. das C. A. M. . Influence of constant lighting, melatonin and geldanamycin under mice testicle over 60 days. Research, Society and Development, [S. l.], v. 10, n. 2, p. e47710212731, 2021. DOI: 10.33448/rsd-v10i2.12731. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/12731. Acesso em: 20 apr. 2024.

Issue

Vol. 10 No. 2

Section

Health Sciences

License

Copyright (c) 2021 Marcos Aurélio Santos da Costa; Anna Carolina Lopes de Lira; Geovanna Hachyra Facundo Guedes; José Anderson da Silva Gomes; Jennyfer Martins de Carvalho; Maria Luísa Figueira de Oliveira; Maria Eduarda da Silva; Bárbara Silva Gonzaga; Pedro Vinícius Silva Novis; Diana Babini Lapa de Albuquerque Britto; Bruno Mendes Tenório; Carina Scanoni Maia; Juliana Pinto de Medeiros; Fernanda das Chagas Angelo Mendes Tenorio

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.