Antibacterial activity of crude extract of Tabernaemontana catharinensis latex (A. DC) against Alicyclobacillus spp.

Authors

DOI:

https://doi.org/10.33448/rsd-v10i9.17907

Keywords:

Antibacterial activity; Tabernaemontana catharinensis (A. DC); Latex; Alicyclobacillus spp.; Orange juice.

Abstract

Alicyclobacillus spp. is composed of Gram-positive, aerobic, thermoacidophilic, endospore-forming bacteria that cause food and beverage spoilage. The presence of Alicyclobacillus spp. may result in the production of guaiacol, which leads to sensory changes in the odour and taste of citrus juices and acidic foods. Tabernaemontana catharinensis (A. DC) is a plant belonging to the family Apocynaceae that produces milky latex with several biological activities described as antioxidant, antiviral, antimicrobial, trypanocidal and anti-leishmanicidal. Therefore, this study aims to evaluate the antimicrobial activity of the crude latex of T. catharinensis (A. DC) against microorganisms of the genus Alicyclobacillus spp. The minimum inhibitory concentration of latex was 7.81 μg/ml for the five Alicyclobacillus species analysed. The minimum bactericidal concentration for the species Alicyclobacillus acidoterrestris 0244T, A. hesperidum 0298T, A. acidiphilus 0247T and A. cycloheptanicus 0297T was 250 μg/ml. Cytotoxicity analysis demonstrated that latex was toxic to Vero cells at concentrations greater than 84.67 μg/ml. Scanning electron microscopy revealed changes in the cell wall of A. acidoterrestris 0244T present in orange juice when treated with crude latex. The results obtained suggest that the crude latex of T. catharinensis (A. DC) displays an antimicrobial effect against Alicyclobacillus, with potential for application in the food industry.

Author Biographies

Márcia Maria dos Anjos Szczerepa , State University of Maringá

Doctor in Food Science, State University of Maringá, Av. Colombo, 5790, Maringá 87020-900, Paraná, Brazil

Kathielle Luiza Mucellini , State University of Maringá

Graduated in Pharmacy, State University of Maringá. Av. Colombo, 5790, Maringá 87020-900, Paraná, Brazil

Érica Benassi Zanqueta , State University of Maringá

Doctor in Pharmaceutical Sciences, State University of Maringá. Av. Colombo, 5790, Maringá 87020-900, Paraná, Brazil.

César Armando Contreras Lancheros , State University of Maringá

Doctor in Pharmaceutical Sciences, State University of Maringá. Av. Colombo, 5790, Maringá 87020-900, Paraná, Brazil.

Tânia Ueda Nakamura , State University of Maringá

Pharmacy Department, State University of Maringá. Av. Colombo, 5790, Maringá 87020-900, Paraná, Brazil.

Celso Vataru Nakamura , State University of Maringá

Pharmacy Department, State University of Maringá. Av. Colombo, 5790, Maringá 87020-900, Paraná, Brazil.

Eliana Harue Endo , State University of Maringá

Doctor in Pharmaceutical Sciences, State University of Maringá. Av. Colombo, 5790, Maringá 87020-900, Paraná, Brazil.

Miguel Machinski Junior , State University of Maringá

Basic Health Science Department, State University of Maringá. Av. Colombo, 5790, Maringá 87020-900, Paraná, Brazil.

Jane Martha Graton Mikcha , State University of Maringá

Department of Clinical Analysis and Biomedicine, State University of Maringá, Av. Colombo, 5790, Maringá 87020-900, Paraná, Brazil.

Regina Aparecida Correia Gonçalves , State University of Maringá

Pharmacy Department, State University of Maringá. Av. Colombo, 5790, Maringá 87020-900, Paraná, Brazil.

Arildo José Braz de Oliveira, State University of Maringá

Pharmacy Department, State University of Maringá. Av. Colombo, 5790, Maringá 87020-900, Paraná, Brazil.

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Published

23/07/2021

How to Cite

RICHARD, F. .; SZCZEREPA , M. M. dos A.; MUCELLINI , K. L.; ZANQUETA , Érica B. .; LANCHEROS , C. A. C. .; NAKAMURA , T. U. .; NAKAMURA , C. V. .; ENDO , E. H. .; MACHINSKI JUNIOR , M.; GRATON MIKCHA , J. M.; GONÇALVES , R. A. C. .; OLIVEIRA, A. J. B. de .; ABREU FILHO, B. A. de. Antibacterial activity of crude extract of Tabernaemontana catharinensis latex (A. DC) against Alicyclobacillus spp. Research, Society and Development, [S. l.], v. 10, n. 9, p. e16310917907, 2021. DOI: 10.33448/rsd-v10i9.17907. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/17907. Acesso em: 4 nov. 2024.

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Agrarian and Biological Sciences