Effect of sucrose on the fatty acid metabolism of adventitious root cultures in vitro of Stevia rebaudiana

Authors

DOI:

https://doi.org/10.33448/rsd-v10i10.18651

Keywords:

Adventitious root cultures; Fatty acids; Stevia rebaudiana; Sucrose.

Abstract

In this study, the effect of sucrose on the neutral lipid profile of adventitious root cultures of Stevia rebaudiana was evaluated. The cultures were obtained employing a roller bottle system. In this system, Schott-type flasks were used, which contained Murashige and Skoog liquid medium at 33.3% strength (MS/3) supplemented with 30, 60, and 80 g L-1 of sucrose, respectively, and 10.7 mM 1-naphthaleneacetic acid (NAA). The spectroscopic analyzes showed that the portion of polyunsaturated fatty acids (PUFAs) was highest in roots treated with 30 g L-1 of sucrose. The spectrometric analyzes showed that the palmitic acid was found to be present in relatively higher amounts in the roots submitted to the MS/3-30 g L-1 (31.9%) and MS/3-60 g L-1 (29.5%) sucrose treatments, and lower in the treatment with MS/3-80 g L-1 (28.8%) of sucrose. Also, the treatment using 30 g L-1 of sucrose was the best for obtaining unsaturated fatty acids (UFAs) in the culture, with a relative percentage of 62.9%. Our results indicate that the MS medium that received 30 g L-1 of sucrose induced a lesser abiotic stress condition, which favored PUFAs production in the adventitious root cultures of S. rebaudiana.

Author Biographies

Bruna Gabrieli Follador, State University of Maringa

Pharmaceutical Sciences Graduate Program, Department of Pharmacy, State University of Maringa, Av. Colombo 5790, 87.020-900, Maringa, Brazil.

Éverton da Silva Santos, State University of Maringa

Pharmaceutical Sciences Graduate Program, Department of Pharmacy, State University of Maringa, Av. Colombo 5790, 87.020-900, Maringa, Brazil.

Regina Aparecida Correia Gonçalves, State University of Maringá

Pharmaceutical Sciences Graduate Program, Department of Pharmacy, State University of Maringa, Av. Colombo 5790, 87.020-900, Maringa, Brazil

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Published

08/08/2021

How to Cite

FOLLADOR, B. G.; SANTOS, Éverton da S. .; GONÇALVES, J. E.; GONÇALVES, R. A. C. .; OLIVEIRA, A. J. B. de . Effect of sucrose on the fatty acid metabolism of adventitious root cultures in vitro of Stevia rebaudiana. Research, Society and Development, [S. l.], v. 10, n. 10, p. e182101018651, 2021. DOI: 10.33448/rsd-v10i10.18651. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/18651. Acesso em: 27 oct. 2021.

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Agrarian and Biological Sciences