By-products of vitiviniculture in the control of downy mildew and induction of defense enzymes in grapevine
DOI:
https://doi.org/10.33448/rsd-v10i11.19908Keywords:
Vitis vinifera; Disease management; Downy mildew; Superoxide dismutase; Peroxidase.Abstract
The preparation of the wines was obtained with a large amount of residues that can be composted with antimicrobial and inductive action. In this sense, the objective of this study was to verify the action of aqueous extract of grape residue (AEGR) on the control of downy mildew and the activation of defense enzymes in cv. Margot. The treatments used were: 12% AEGR, Saccharomyces cerevisae (Agro-Mos®), Bordeaux mixture (BM) and water (control). A germination test of Plasmopara viticola, area under the disease progression curve (UDPC) of downy mildew in leaf discs, grapevine plants under greenhouse conditions and organic vineyard were performed. The activity of the enzymes superoxide dismutase (SOD) and peroxidase (POD) on leaves of grapevines was determined. AEGR reduces the germination of P. viticola by 58%, 99.3% and 50% the UDPC of the downy mildew in leaf discs and in greenhouse plants, respectively. However, in the field the AEGR did not present statistical difference with the control. It was observed that in greenhouse plants SOD was similar for control and AEGR and in the field this enzyme showed no activity. POD showed an increase of 60.34% in the plants treated with AEGR in the greenhouse and 45% in the field. The AEGR presents potential for the control of downy mildew and activation of cv. Margot, but it is necessary to optimize these effects in plants under field conditions.
References
Andreasson, E., Marit, L. & Laith M. (2017). Plant extracts comprising at least a phenolic compound, for inducing the natural defense of a plant against a pathogen, such as Phytophthora infestans. United States Patent, 9, 669-681.
Ayoade, J. O. Introdução a climatologia para os trópicos (1998). trad. Maria Juraci Zani dos Santos. Bertrand Brasil, 350p.
Azevedo, L. A. S. (1997). Manual de quantificação de doenças de plantas. Novartis Biociências- Setor Agro, 114p.
Baxter, A., Mittler, R. & Uzuki, N. (2014). ROS as key players in plant stress signalling. Journal Experimental Botany, 65(5), 1229–1240.
Benouaret, R., Goujon, E. & Goupil, P. (2014). Grape marc extract causes early perception events, defence reactions and hypersensitive response in cultured tobacco cells. Plant Physiology and Biochemistry, 77, 84-89.
Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72 (1-2), 248–254.
Costa, M. J., Zambolim, L. & Rodrigues, F. A. (2007). Avaliação de produtos alternativos no controle da ferrugem do cafeeiro. Fitopatologia Brasileira, 32 (2), 150-155.
Diniz, I., Azinheira, H., Figueiredo, A., Gichuru, E., Oliveira, H., Guerra-Guimaraes, L. & Silva, M. C. (2019). Fungal penetration associated with recognition, signaling and defence-related genes and peroxidase activity during the resistance response of coffee to Colletotrichum kahawae. Physiological and Molecular Plant Pathology, 105, 119-127.
Ferreira, D. F. (2011). Sisvar: a computer statistical analysis system. Ciência e Agrotecnologia, 35, 1039-1042.
Fesel, P.H. & Zuccaro, A. (2016). β-glucan: Crucial component of the fungal cell wall and elusive MAMP in plants. Fungal Genetics and Biology, 90, 53-60.
Garcia, C., Fedrigo, K., Gabriel, A., Botelho, R. V., Rodrigues, J. D., & Ono, E. O. (2021). Control of mildew in vines with cinnamon extract and catalase activity in organic production. Research, Society and Development, 10, 1-10.
Gabaston, J., Richard, T., Biais, B., Waffo-Teguo, P., Pedrot, E., Jourdes, M., & Mérillon, J. M. (2017). Stilbenes from common spruce (Picea abies) bark as natural antifungal agent against downy mildew (Plasmopara viticola). Industrial Crops and Products, 103, 267-273.
Gauthier, A., Trouvelot, S., Kelloniemi, J., Frettinger, P., Wendehenne, D., Daire, X., & Poinssot, B. (2014). The sulfated laminarin triggers a stress transcriptome before priming the SA-and ROS-dependent defenses during grapevine's induced resistance against Plasmopara viticola. PLoS One, 9, e88145.
Giannopolitis, C. N. & Ries, S. K. (1977). Superoxide dismutases. I. Occurrence in higher plants. Plant Physiology, 59, 309-314.
Kim, N., Kim, J. K, Hwang, D, & Lim, Y. H. (2013). The possible mechanism of rhapontigenin influencing antifungal activity on Candida albicans. Medical mycology, 51, 45-52.
Koh, J. C., Barbulescu, D. M., Salisbury, P. A., & Slater, A. T. (2016). Pterostilbene is a potential candidate for control of blackleg in canola. PLoS One, 11, e0156186.
Lusso, M. F. G. & Pascholati, S. F. (1999). Activity and isoenzymatic pattern of soluble peroxidases in maize tissues after mechanical injury or fungal inoculation. Summa Phytopathologica, 25, 244-249.
Madureira, J., Barros, L., Melo, R., Verde, S. C., Ferreira, I. C., & Margaça, F. M. A. (2018). Degradation of phenolic acids by gamma radiation as model compounds of cork wastewaters. Chemical Engineering Journal, 341, 227-237.
Martínez, G., Regente, M., Jacobi, S., Del Rio, M., Pinedo, M., & De La Canal, L. (2017). Chlorogenic acid is a fungicide active against phytopathogenic fungi. Pesticide Biochemistry and Physiology, 140, 30-35.
Ministério da agricultura, pecuária e abastecimento. MAPA-2018. Projeções do agronegócio, 2018. http://www.agricultura.gov.br/assuntos/politica-agricola/todas-publicacoes-de-politica-agricola/projecoes-doagronegocio/PROJECOES2018_FINALIZADA_web_050 92018.pdf?fbclid=I wAR1E_suqSIA OOAtv1bGME1J4Bab5uNbnz6l2uwBoaBZIpZWHA2kKyBYQotA
Pereira, A. S., Shitsuka, D. M., Parreira, F. J., & Shitsuka, R. (2018). Metodologia da pesquisa científica. UFSM. 5.3.
Pereira, D. T. V., Tarone, A. G., Cazarin, C. B. B., Barbero, G. F. & Martínez, J. (2019). Pressurized liquid extraction of bioactive compounds from grape marc. Journal of Food Engineering, 240, 105-113.
Richard, T., Abdelli-Belhad, A., Vitrac, X., Waffo-Téguo, P., & Mérillon, J. M. (2016). Vitis vinifera canes, a source of stilbenoids against downy mildew. Oeno One, 50, 137-143.
Shaner, G. & Finney, R. (1977). The effect of nitrogen fertilization on the expression of slow mildewing resistance in Knox Wheat. Journal of Phytopathology, 67, 1051-1056.
Teixeira, A., Baenas, N., Dominguez-Perles, R., Barros, A., Rosa, E., Moreno, D. A., & Garcia-Viguera, C. (2014). Natural bioactive compounds from winery by-products as health promoters: A review. International Journal of Molecular Sciences, 15, 15638-15678.
Tröster, V., Setzer, T., Hirth, T., Pecina, A., Kortekamp, A. & Nick, P. (2017). Probing the contractile vacuole as Achilles’ heel of the biotrophic grapevine pathogen Plasmopara viticola. Protoplasma, 254, 1887-1901.
Yadav, S., Gill, S. S, Passricha, N., Gill, R., Badhwar, P., Anjum, N. A., & Tuteja, N. (2019). Genome-wide analysis and transcriptional expression pattern-assessment of superoxide dismutase (SOD) in rice and Arabidopsis under abiotic stresses. Plant Gene, 17, 1-9.
Zanardo, N. M. T, Pascholati, S. F, & Fialho, M. B. (2010). Resistência de plântulas de pepineiro a Colletotrichum lagenarium induzida por frações de extrato de Saccharomyces cerevisiae. Pesquisa Agropecuária Brasileira, 44, 1499-1503.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Marcos Vinicius Horst; Carla Daiane Leite; Carla Garcia; Aline José Maia; Cacilda Márcia Duarte Rios Faria; Renato Vasconcelos Botelho

This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.