Biotechnology: identification and evaluation of the Bacillus cereus amylolytic activity

Authors

DOI:

https://doi.org/10.33448/rsd-v10i13.21301

Keywords:

Amylase; Bacillus; Bioprocess; Biotechnology; Enzymes.

Abstract

Biotechnology is the branch of science that uses molecules, viruses, microorganisms, cells, animals, plants or part of them in technological processes to generate benefits for humans, to flora, fauna and the environment. In this context, enzymes are natural biocatalysts that present substrate specificity and extreme importance to vital processes, as they develop indispensable functions in biochemical reactions of cell metabolism, and can be used in biotechnological processes. Knowing the human needs, for a world population of about 7.7 billion people and the functional usefulness of enzymes, there is, on the one hand, a gigantic demand for the consumption of various products in the agricultural sector, processed and industrialized, such as: food, beverages, clothing in the textile sector, medicines, vaccines, cosmetics in the chemical-pharmaceutical sector, as well as in the production of paper and fuels, in which enzymes, mainly amylases, have been widely used in production processes. In this work, experiments were carried out with the wild Bacillus cereus bacterium to verify the production of amylases, the results obtained could demonstrate the formation of amylolysis halos around the colonies in Petri dishes containing Tryptic Soy Agar + starch (1%) medium, pH 7.3 and grown in a biological oven at 37oC for 24 hours, when revealed in iodine vapor; the Amylolysis Index (AI) was 3.3 and the efficiency of starch substrate degradation by amylases was greater than 90% in the evaluated treatments.

Author Biographies

Jefferson Alves da Costa Junior, Universidade Federal de Santa Maria

Prof. Dr. Programa de Pós-Graduação em Ciência e Tecnologia Ambiental, Universidade Federal de Santa Maria, Campus de Frederico Westphalen - RS, Brasil

Genesio Mario da Rosa, Universidade Federal de Santa Maria

Prof. Dr. Programa de Pós-Graduação em Ciência e Tecnologia Ambiental, Universidade Federal de Santa Maria, Campus de Frederico Westphalen - RS, Brasil

Arci Dirceu Wastowski , Universidade Federal de Santa Maria

Prof. Dr. Programa de Pós-Graduação em Ciência e Tecnologia Ambiental, Universidade Federal de Santa Maria, Campus de Frederico Westphalen - RS, Brasil.

Hilda Hildebrand Soriani, Universidade Federal de Santa Maria

Prof. Dr. Programa de Pós-Graduação em Ciência e Tecnologia Ambiental, Universidade Federal de Santa Maria, Campus de Frederico Westphalen - RS, Brasil.

Ana Paula Corteze Locatelli, Universidade Federal de Santa Maria

MsC-academic/Programa de Pós-Graduação em Ciência e Tecnologia Ambiental, Universidade Federal de Santa Maria, Campus de Frederico Westphalen - RS, Brasil

Darlan Weber da Silva, Universidade Federal de Santa Maria

MsC-Academic/Programa de Pós-Graduação em Ciência e Tecnologia Ambiental, Universidade Federal de Santa Maria, Campus de Frederico Westphalen - RS, Brasil

Deisy Brasil Gonçalves, Universidade Federal de Santa Maria

MsC- academic/Programa de Pós-Graduação em Ciência e Tecnologia Ambiental, Universidade Federal de Santa Maria, Campus de Frederico Westphalen - RS, Brasil

Gabriel Baraldi Volpi , Universidade Federal de Santa Maria

MsC-academic/Programa de Pós-Graduação em Ciência e Tecnologia Ambiental, Universidade Federal de Santa Maria, Campus de Frederico Westphalen - RS, Brasil. 

Isabela Schamann Konzen , Universidade Federal de Santa Maria

Sci. Init. Academic - Curso de Engenharia Ambiental e Sanitária, Universidade Federal de Santa Maria, Campus de Frederico Westphalen - RS, Brasil.

Kauane Andressa Flach , Universidade Federal de Santa Maria

MsC- academic/Programa de Pós-Graduação em Ciência e Tecnologia Ambiental, Universidade Federal de Santa Maria, Campus de Frederico Westphalen - RS, Brasil.

Ubiratan Alegransi Bones , Universidade Federal de Santa Maria

MsC-academic/Programa de Pós-Graduação em Ciência e Tecnologia Ambiental, Universidade Federal de Santa Maria, Campus de Frederico Westphalen - RS, Brasil.

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Published

18/10/2021

How to Cite

COSTA JUNIOR, J. A. da .; ROSA, G. M. da; WASTOWSKI , A. D.; SORIANI, H. H. .; LOCATELLI, A. P. C. .; SILVA, D. W. da; GONÇALVES, D. B. .; VOLPI , G. B. .; KONZEN , I. S. .; FLACH , K. A. .; BONES , U. A. . Biotechnology: identification and evaluation of the Bacillus cereus amylolytic activity . Research, Society and Development, [S. l.], v. 10, n. 13, p. e437101321301, 2021. DOI: 10.33448/rsd-v10i13.21301. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/21301. Acesso em: 14 nov. 2024.

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Agrarian and Biological Sciences