Panagrellus redivivus: a promising nematode for cell biology practical classes
DOI:
https://doi.org/10.33448/rsd-v11i2.25629Keywords:
Multicellularity; Cell theory; Cytology practice; Embryogenesis.Abstract
Cell theory condenses studies on the origin, composition and functioning of cells. The first contributors to this theory were Matthias Schleiden and Theodor Schwann who, through their studies, concluded that all living beings are made up of cells. The understanding of basic concepts of cell biology is fundamental for the learning of physiology, reproduction, heredity, propagation of diseases and interactions between living beings. Therefore, from elementary school to high school, science subjects cover cytology topics. However, these are treated in a predominantly theoretical way. Practical classes are extremely important for scientific literacy and should be carried out from basic education onwards. However, there is a lack of experiments that allow the visualization of animal cells in living tissue. In view of this, this article proposes a practical class using the worm Panagrellus redivivus in order to elucidate multicellularity as well as the formation of new cells and the living being through embryogenesis. This nematode is easy to obtain and handling is done using low-cost materials, but the learning gain is very high. It is believed that the use of this experimental model not only facilitates the understanding of the proposed topic but also helps and encourages the scientific training of students.
References
Abolafia, J., Alizadeh, M., & Khakvar, R. (2016). Description of Panagrellus ulmi sp. n. (Rhabditida, Panagrolaimidae) from Iran, and comments on the species of the genus and its relatives. Zootaxa, 4162(2), 245–267.
Alberts, B., Bray, D., Hopkin, K., Johnson. A., Lewis, J., Raff, M. ... Walter, P. A. (2017). Fundamentos da Biologia Celular. Porto Alegre, Brasil: Artmed.
Borges, T. S., & Alencar G. (2014). Metodologias ativas na promoção da formação crítica do estudante: o uso das metodologias ativas como recurso didático na formação crítica do estudante do ensino superior. Cairu em Revista, 3(4), 119-143. Recuperado de https://www.cairu.br/revista/arquivos/artigos/2014_2/08%20METODOLOGIAS%20ATIVAS%20NA%20PROMOCAO%20DA%20FORMACAO%20CRITICA%20DO%20ESTUDANTE.pdf
Costa, J. L., & Frenedozo, R. C. (2020). Metodologias ativas nas atividades investigativas em aulas de Biologia. (Dissertação de Mestrado). Universidade Cruzeiro Do Sul, São Paulo, Brasil.
Fernandes, M. G., Vaini, J. O., Crispim. B. A., & Teixeira, T. Z. (2017). Práticas de biologia celular. Dourados, Brasil: UFGD.
Ferris, H. (2012). The beer mat nematode, Panagrellus redivivus: A study of the connectedness of scientific Discovery. Journal of Nematode Morphology and Systematics, 12(1), 19-25.
Focken, U., Schlechtriem, C., Wuthenau, M., García-Ortega, A., Puello-Cruz, A., & Becker, C. (2006, 08 de setembro). Massa de Panagrellus redivivus produzida em meio sólido como alimento vivo para larvas de Litopenaeus vannamei. Aquaculture Research, 37(14), 1429-1436.
Freitas, D. F., (2017). Caracterização e produção de melanina pelo fungo Nematófago duddingtonia flagrans e avaliação da sua participação na atividade predatória (Tese de Doutorado). Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Brasil.
Gaspar, A (2015). Experiências de Ciências para o Ensino Fundamental. São Paulo, Brasil: Ática.
Hechler, H. C., (1970). Reproduction, Chromosome Number, and Postembryonic Development of Panagrellus redivivus (Nematoda: Cephalobidae). Journal of Nematology, 2(4), 355-361.
Ivanova, E., Perfilieva, K., & Spiridonov, S. (2018). Panagrellus levitatus sp. n. (Rhabditida: Panagrolaimidae), a nematode suppressing Drosophila melanogaster in laboratory cultures. Nematology, 20(3), 285-297.
Lavens, P., & Soorgelos, P. (1996). Manual on the production and use of live food for aquaculture. Roma, Itália: FAO.
Morán, J. (2015). Mudando a educação com metodologias ativas (Coleção Mídias Contemporâneas. Convergências Midiáticas, Educação e Cidadania: aproximações jovens). Ponta Grossa: UEPG/PROEX.
Nadler, S.A., Ley, P., Mundo-Ocampo, M., Smythe, A. B., Stock, S. P., Bumbarger, D. ... Baldwin, J.G. (2006). Phylogeny of Cephalobina (Nematoda): Molecular evidence for recurrent evolution of probolae and incongruence with traditional classiWcations. Molecular Phylogenetics and Evolution, 40(3), 696-711.
Oliveira, M. A. F., Andrade, L. C. R., & Araújo V. R. (2019, outubro). Reflexões sobre a importância das práticas de microscopia no conteúdo de biologia celular no ensino médio. In Anais do VI Congresso Nacional de Educação. Fortaleza, Brasil.
Quesado, H. L. C., Cavalcante, M. P. P., & Menezes, M. F. F. (1996). Biologia práticas. Fortaleza, Brasil: EUFC.
Radwin, I. A., & Rouse, D. B. (1990). Communications: Yield characteristics of the free-living nematode Panagrellus redivivus in different culture media. The Progressive Fish-Culturist, 52(4), 237-240.
Sautter, J., Kaiser, H., Focken, U., & Becker, K. (2007, 3 de maio). Panagrellus redivivus (Linné) as a live food organismin the early rearing of the catfish Synodontis petricola (Matthes). Aquaculture Research, 38(6), 653-659.
Souza, A. C. (2013). A experimentação no ensino de ciências: importância das aulas práticas no processo de ensino aprendizagem (Monografia de Especialização). Universidade Tecnológica Federal do Paraná, Medianeira, Brasil.
Stock, S.P., & Nadler, S. A. (2006). Morphological and molecular characterisation of Panagrellus spp. (Cephalobina: Panagrolaimidae): taxonomic status and phylogenetic relationships. Nematology, 8(6), 921-938.
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