Characterization of the biochemical response of Coffea canephora accessions regarding resistance to orange rust

Authors

DOI:

https://doi.org/10.33448/rsd-v11i7.30171

Keywords:

Enzyme activity; Coffee; Clones; Genetical enhancement.

Abstract

In situations of abiotic or biotic stress, plants tend to activate defense responses, whether structural or biochemical. The objective of this work was to study the interaction of clones of Coffea canephora x Hemileia vastatrix, to identify the host responses in the interaction of incompatibility and compatibility with the pathogen. For that, seedlings with 120 days of age of the clones of the variety BRS 'Ouro Preto' C199, C160 and C125, were inoculated with the pathogen (1.5 x 104 urediniospores/mL) and the plants were taken to the inoculation chamber at 25 °C and 90% relative humidity for up to 168 hours. The data were submitted to analysis of variance and the means were compared by Tukey test at 1% of significance. After the quantification of total proteins, it is possible to observe that in Clones C199 and C160 the first increase in protein content occurred after six hours, with the maximum production peak occurring after 72 hours. And this dispersing slightly only after 120 hours. In the susceptible clone, the response only occurred after 48 hours, which was 20% lower than the resistant variety and there was no second peak of response, of production. For the activities of peroxidase and phenylalanine-ammonia-lyase, a similar response pattern was observed for the clones under study, where an induction of activity and its intensity were higher in these resistant clones. This result suggests that there is a biochemical response involved in rust resistance and this should be especially investigated at 72 hours after inoculation.

Author Biographies

Aline Souza da Fonseca, Universidade Federal de Rondônia

Biologist, Master in Environmental Sciences and Doctoral Student in Biodiversity and Biotechnology at the Bionorte-Federal University of Rondônia -UNIR, Porto Velho (RO) Brazil.

Tamiris Chaves Freire , Universidade Federal de Rondônia

Biologist, Master in Environmental Sciences and Doctoral Student in Biodiversity and Biotechnology at the Bionorte-Federal University of Rondônia -UNIR, Porto Velho (RO) Brazil.

Jessica Silva Felix Bastos, Universidade Federal de Rondônia

Biologist, Master in Environmental Sciences and Doctoral Student in Biodiversity and Biotechnology at the Bionorte-Federal University of Rondônia -UNIR, Porto Velho (RO) Brazil.

Simone Carvalho Sangi, Universidade Federal de Rondônia

Biologist, Master in Environmental Sciences and Doctoral Student in Biodiversity and Biotechnology at the Bionorte-Federal University of Rondônia -UNIR, Porto Velho (RO) Brazil.

Liliani Ogrodowczyk, Universidade Federal de Rondônia

Pharmacist, Master in Environmental Sciences, Federal University of Rondônia -UNIR, Porto Velho (RO) Brazil.

Rodrigo Barros Rocha, Empresa Brasileira de Pesquisa Agropecuária

Biologist, PhD in Genetics and Improvement from the Federal University of Viçosa, Researcher at the Brazilian Agricultural Research Corporation of Embrapa Rondônia, Brazil.

Marcelo Curitiba Espindula, Empresa Brasileira de Pesquisa Agropecuária

Agronomist, Doctor in Plant Science from the Federal University of Viçosa, Researcher at the Brazilian Agricultural Research Corporation of Embrapa Rondônia, Brazil.

José Roberto Vieira Junior, Empresa Brasileira de Pesquisa Agropecuária

Agronomist, PhD in Plant Pathology from the Federal University of Viçosa, Researcher at the Brazilian Agricultural Research Corporation, Brazil.

Cléberson de Freitas Fernandes , Empresa Brasileira de Pesquisa Agropecuária

Pharmacist-biochemist, PhD in Biochemistry from the Federal University of Ceará, Researcher at the Brazilian Agricultural Research Corporation, Brazil.

References

Alfonsi, W. M. V., Coltri, P. P., Zullo Jr., J., Patrício, F. R. A. & Alfonsi, E. L. (2019). Período de incubação da ferrugem do cafeeiro. Summa phytopathol. 45(2), 134-140. https://doi.org/10.1590/0100-5405/187216.

Bradford, M. M. (1976). A rapid and sensitive method for the quantification of microgram quantities of proteins utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-254, 1976.

CONAB. (2020). Acomp. safra brasileira de café, v. 6 – Safra 2020, n.1- Primeiro levantamento. CONAB – Companhia Nacional de Abastecimento. Brasília, p. 1-62. https://www.conab.gov.br/info-agro/safras/cafe.

CONAB. (2022). Acomp. safra brasileira de café, v. 9 – Safra 2022, n.1- Primeiro levantamento. CONAB – Companhia Nacional de Abastecimento. Brasília, p. 1-60. https://www.conab.gov.br/info-agro/safras/cafe.

Dhingra, O. B. & Sinclair, J. B. (1995). Basic Plant Pathology Methods. 2nd Edition, CRC Press, Boca Raton.

Galeano, R. M. S., Campelo, A. P. S., Mackert, A., & Brasil, M.S. (2019). Desenvolvimento inicial e quantificação de proteínas do milho após inoculação com novas estirpes de Azospirillum brasilense. Agricultura Neotropical. 6(2) 95-99. https://doi.org/10.32404/rean.v6i2.2613

Horst, M. V., Leite, C. D., Garcia, C., Maia, A. J., Faria, C. M. D. R. & Botelho, R. V. (2021). Subproduto da vitivinicultura no controle do míldio e indução de enzimas de defesa em videira. Research, Society and Development, 10(11). http://dx.doi.org/10.33448/rsd-v10i11.19908

Karanastasi, E., Kostara, T., Malamos, N. & Zervoudakis G. (2018). Catalase activity, lipid peroxidation, and protein concentration in leaves of tomato infected with meloidogyne javanica. Nematropica. 48(1). file:///C:/Users/Profissional/Downloads/admin-final-galley-ms-648-karanastasi-pages-15-20%20.pdf

Kinnear, P. R. & Gray, C. D. (2000). SPSS for windows made simple. Department of Psychology, University of Aberdeen. United Kingdom.

Macagnan, D., Romeiro, R. S., Baracat-Pereira, M. C., Lanna-Filho, R., Batista, G. S. & Pomella, A.W.V. (2008). Atividade de enzimas associadas ao estado de indução em mudas de cacaueiro expostas a dois actinomicetos residentes de filoplano. Summa Phytopathologica, 34(1) 34-37. https://www.scielo.br/j/sp/a/g6dVyh8XqrDSVfKdL9Z9tgR/?lang=pt

MAPA. (2022). Ministério da Agricultura, Pecuária e Abastecimento. Agronegócio brasileiro: balança comercial do agronegócio – janeiro/2022. https://static.poder360.com.br/2022/02/exportacoes-agro-jan2022.pdf

Melo, G. A., Shimizu, M. M. & Mazzafera, P. (2006). Polyphenoloxidase activity in coffee leaves and its role in resistance against the coffee leaf miner and coffee leaf rust. Phytochemistry. 67(3), 277–285. https://doi.org/10.1016/j.phytochem.2005.11.003

Menezes-Silva, P. E., Sanglard, L. M. V. P., Ávila, R. T., Morais, L. E, Martins, S. C. V, Nobres, P. & Matta, F. M. (2017). Photosynthetic and metabolic acclimation to repeated drought events play key roles in drought tolerance in coffee. Journal of Experimental Botany, 68(15), 4309–4322. https://doi.org/10.1093/jxb/erx211

Mori, T., Sakurai, M. & Sakuta, M. (2001). Effects of conditioned medium on activities of PAL, CHS, DAHP synthase (DS-Co and DS-Mn) and anthocyanin production in suspension cultures of Fragaria ananassa. Plant Science. 160(2), 355–360. https://doi.org/10.1016/s0168-9452(00)00399-x

Pérez-Àlvarez, S., Héctor-Ardisana, E. F., Escobedo-Bonilla, C. M., Flores-Córdova, M. A., Sánchez-Chávez, E. & García, C. U. (2021). Actividad bioquímica y molecular de enzimas del estrés oxidativo en plantas detomate creciendo con plomo. Ecosistemas y recursos agropecuários, 8(3). https://doi.org/10.19136/era.a8n3.3163

Pinto, M. S. T., Ribeiro, J. M. & Oliveira, E. A. G. (2011). O estudo de genes e proteínas de defesa em plantas. Revista Brasileira de Biociências. 9(2), 241-248. http://www.ufrgs.br/seerbio/ojs/index.php/rbb/article/view/1732

Piza, I. M. T., Lima, G. P. P. & Brasil, O. G. (2003). Atividade de peroxidase e níveis de proteínas em plantas de abacaxizeiro micropropagadas em meio salino. Revista Brasileira de Agrociência. 9(4), 361-366. https://periodicos.ufpel.edu.br/ojs2/index.php/CAST/article/view/661/659

Rahman, M. & Punja, Z. K. (2005). Biochemistry of ginseng root tissues affected by rusty root symptoms. Plant Physiology and Biochemistry. 43(12), 1103-1114. https://doi.org/10.1016/j.plaphy.2005.09.004

Ryan, C. A. (2000). The systemin signaling pathway: differential activation of plant defensive genes. Biochimica et Biophyica Acta. 1477 (1–2), 112-121. https://doi.org/10.1016/S0167-4838(99)00269-1

Silva, M. C., Nicole, M., Guerra-Guimarães, L. & Rodrigues J. R. (2002). Hypersensitive cell death and post-haustorial defence responses arrest the Orange rus (Hemileia vastatrix) growth in resistant coffe leaves. Physiological and Molecular Plant Pathology. 60(4), 169-183. https://doi.org/10.1006/pmpp.2002.0389

Silva, L. C., Silva, I. S., Souza, P. C., Vieira, R. J., Oliveira, D. C., Costa, L. G. & Campos, D. S. (2018). Distribuição espacial da ferrugem do cafeeiro (Hemileia vastatrix) em uma lavoura do IFMG, campus Bambuí/MG. http://www.sbicafe.ufv.br/handle/123456789/11673

Talhinhas, P., Batista, D., Diniz, I., Vieira, A., Silva, D. N., Loureiro, A, Tavares, S., Pereira, A, P. Azinheira, H. G., Guerra-Guimaraes, L., Varzea ,V. & Silva, M. C. (2017). The coffee leaf rust pathogen Hemileia vastatrix: one and a half centuries around the tropics. Molecular plant pathology. 18(8), 1039 –1051. https://doi.org/10.1111/mpp.12512

Urbanek, H., Kuzniak-Gebarowska, E. & Herka, K. (1991). Elicitation of defense responses in bean leaves by Botrytis cinerea polygalacturonase. Acta Physiologiae Plantarum, 13(1), 43-50

Zambolim, L. (2016). Current status and management of coffee leaf rust in Brazil. Tropical Plant Pathology, 41(1), p.1-8. https://doi.org/10.1007/s40858-016-0065-9

Published

04/06/2022

How to Cite

FONSECA, A. S. da .; FREIRE , T. C. .; BASTOS, J. S. F. .; SANGI, S. C. .; OGRODOWCZYK, L.; ROCHA, R. B. .; ESPINDULA, M. C. .; VIEIRA JUNIOR, J. R.; FERNANDES , C. de F. . Characterization of the biochemical response of Coffea canephora accessions regarding resistance to orange rust. Research, Society and Development, [S. l.], v. 11, n. 7, p. e56211730171, 2022. DOI: 10.33448/rsd-v11i7.30171. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/30171. Acesso em: 6 jul. 2022.

Issue

Section

Agrarian and Biological Sciences