Technological Potential of Non-Saccharomyces yeasts
DOI:
https://doi.org/10.33448/rsd-v11i10.32754Keywords:
Yeasts; Non-Saccharomyces; Fermentation processes.Abstract
Beer is a beverage made up of water, malt, hops and yeast. Yeast is the main transforming agent, converting sugars into ethanol, CO2 and a series of compounds providing flavor and aroma. Saccharomyces yeasts stand out as the most used by the industry. With the growing number of beer consumers and the great diversity of flavors, the number of breweries is also increasing. The objective of this research was to evaluate the biotechnological potential of non-Saccharomyces yeasts kept in collections. The biotechnological potential to be used in the industry was evaluated. Biochemical differentiation of strains into ALE and LAGER, ethanol tolerance and yeast flocculation potential were performed. The results obtained showed that the yeasts presented a potential to be applied in fermentation processes. All yeasts used in the biochemical differentiation tests showed results that allow classifying them as LAGER type yeasts. In the flocculation test, all yeasts were found to be industrial yeasts according to the classification criteria of the American Society of Brewing Chemistry. In the evaluation of ethanol tolerance at concentrations of 10%, 15% and 20%, only the yeast used in the control showed tolerance to 15% ethanol, the yeast Saccharomyces cerevisiae obtained from the culture collection showed tolerance to 10% ethanol, and the others did not. showed tolerance to ethanol at the concentrations used. The study presented promising results suggesting that yeasts can be used in fermentation processes, being necessary to carry out further investigations to certify the real potential of these yeasts.
References
Alves jr, S. L., Herberts, R. A., Hollatz, C., Trichez, D., Milleti, L. C., Araujo, P. S., & Stambuk, B. U. (2008). Molecular analysis of maltotriose active transport and fermentation by Saccharomyces cerevisiae revels a determinant role for the AGT1 permease. Applied and Environmental Microbiology ,74, 1494-1501. 10.1128/AEM.02570-07
Andrietta, S. R., Serra, G. E., & Andrietta, M. G. S. (1999). Classificação das linhagens de leveduras de processos industriais de fermentação alcoólica utilizando capacidade fermentativa. STAB, açúcar, álcool e subprodutos, 17,54-59.
Araujo. T. M. (2013). Caracterização Bioquímico-molecular de Cepas de Saccharomyces cerevisiae Isoladas de Dornas de Fermentação de Cachaça para Produção de Cervejas. Dissertação Mestrado em Biotecnologia NUPEB. Universidade Federal de Ouro Preto. Ouro Preto, Minas Gerais.
ASBC (1992) ASBC Methods of Analysis (8th ed.), American Society of Brewing
Basso, R. F. (2019). Caracterização de leveduras não convencionais para produção de cervejas. Dissertação Mestrado em Ciência e Tecnologia de Alimentos Universidade de São Paulo Escola Superior de Agricultura "Luiz de Queiroz", Piracicaba. São Paulo.
Boulton, C. (2017) Brewing Yeast Physiology. Brewing Microbiology: Current Research, Omics and Microbial Ecology, Caister Academic Press, 1-28. 10.21775/9781910190616.01.
Brasil. (2020). Anuário da Cerveja. Ministério da Agricultura, Pecuária e Abastecimento-MAPA. http://www.cervbrasil.org.br/novo_site/http-www-cervbrasil-org-br-novo_site-wp-content-uploads-2021-04-anuariocerveja2-pdf/.
D’hautcourt, O. & Smart, K. A. (2018). Measurement of brewing yeast flocculation. Journal of American Society of Brewery Chemistry. 57: 123-128. doi: 10.1094/ASBCJ-57-0123.
Gallone, B., Mertens, S., Crauwelse, S., Lievense.B., Verstrepen, K. J., & Steesels, J. (2017). Genomics and Evolution of Beer Yeasts. Brewing Microbiology: Current Research, Omics and Microbial Ecology, Caister Academic Press, 145-178. 10.21775/9781910190616.06
Giorgi, V. V., & Júnior, J. O. C. (2016) A produção de cervejeira como patrimônio intangível. Cultura Histórica & Patrimonio.
Hatoum, R., Labrie, S., & Fliss, I. (2012). Antimicrobial and probiotic properties of yeasts: from fundamental to novel applications. Frontiers in Microbiology. (a.421), 1-12. 10.3389/fmicb.2012.00421.
Mukherjee, V., Radecka, D., Aerts, G., Verstrepen, K. J., Lievens, B., & Thevelein, J. M. (2017). Phenotypic landscape of non-conventional yeast species for different stress tolerance traits desirable in bioethanol fermentation. Biotechnology for biofuels, 10, 216. 10.1186/s13068-017-0899-5
Müller, M., Bellut, K., Tippmann, J., & Becker, T. (2017), Physical Methods for Dealcoholization of Beverage Matrices and their Impact on Quality Attributes. ChemBioEng Reviews, 4, 5,310–326. 10.1002/cben.201700010
Naumova, E. S., Serpova, E. V., Korshunova, I. V., & Naumov, G. I. (2011). Molecular polymorphism of α-galactosidase MEL genes of Saccharomyces yeasts. Microbiology, 80(4), 502–513. doi:10.1134/s0026261711040151
Parapouli, M., Vasileiadis, A., Afendra, A. S., & Hatziloukas E. (2020). Saccharomyces cerevisiae and its industrial applications. AIMS Microbiol, 6(1),1-31.10.3934/microbiol.2020001.
Sampaio, J. P., Pontes, A., Libkind, D., & Hutzler, M. (2017). Taxonomy, Diversity, and Typing of Brewing Yeasts. Brewing Microbiology: Current Research, Omics and Microbial Ecology, Caister Academic Press, 85-118. 10.21775/9781910190616.04
Sicard, D., & Legras, J. L. (2011). Bread, beer and wine: Yeast domestication in the Saccharomyces sensu stricto complex. Compts Rendus Biologies. 334(3):229-36. 10.1016/j.crvi.2010.12.016.
Smart, K. A. (2017). Yeast Stress and Brewing Fermentations. Brewing Microbiology: Current Research, Omics and Microbial Ecology, Caister Academic Press, 29-52. 10.21775/9781910190616.02.
Stewart, G. G., & Russel, I. (1998). An introduction to brewing Science & Technology: series III: brewer´s yeast. London: The Institute of brewing.
Varela C: (2016). The impact of non-Saccharomyces yeasts in the production of alcoholic beverages. Appl Microbiol Biotechnol, 100(23), 9861-9874. 10.1007/s00253-016-7941-6
Verstrepen, K. J., Derdelinckx, G., Verachtert, H., & Delvaux, F. R. (2003). Yeast flocculation: What brewers should know. Applied Microbiology and Biotechnology, 61(3), 197-205. 10.1007/s00253-002-1200-8.
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Copyright (c) 2022 Pérsio Alexandre da Silva; Ninive Bezerra Florêncio; Gleyka Daisa de Melo Santos; Erik Jonne Vieira de Melo; Norma Buarque de Gusmão
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