Synthesis of single cell oil from crude glycerol: a systematic literature review

Authors

DOI:

https://doi.org/10.33448/rsd-v11i16.38071

Keywords:

Biodisel; Fungi; Yeasts; Oil; Transesterification.

Abstract

Biodiesel has been an important energy alternative for reducing the emission of polluting gases, however, vegetable oils and animal fats used as raw materials for its synthesis have restrictions that limit the production process. This work is a systematic literature review that discusses possible applications for the reuse of crude glycerol from the transesterification of vegetable oil or animal fat from biodiesel production. Thus, we present some microorganisms that may be used in the production of microbial oil (single cell oil) from crude glycerol. The yeasts Rhodotorula mucilagenosa and Rhodosporidium toruloides, with lipid content of 65% and 64%, respectively, and the filamentous fungus Mortierella isabellina with 66%, are potential producers of single cell oil. In M. isabelina, the stored lipids are mostly neutral, in addition, its total composition of fatty acids presents remarkable similarities with Brassica napus oil, an excellent oil for biodiesel production. The fatty acid profile for R. mucilagenosa is rich in oleic acid (61.88%), linoleic acid (16.17%), and linolenic acid (1.03%) comprising ~80% monounsaturated fatty acids and fatty acids polyunsaturated fats from total lipids. In R. toruloides, the fatty acid profiles of the lipids are similar to Jatropha curcas oil, a raw material widely used for the production of biodiesel. Overall, it is expected that this review will stimulate the development of new processes, mainly for the destination of crude glycerol from the biofuel chain in single cell oil.

References

Alves, A. P., Filho, G. M. R., & Mendes, M. F. (2017). Avaliação técnica de diferentes processos de separação para purificação do glicerol como subproduto. Revista Brasileira de Energias Renováveis, 6(5), 955–982. http://dx.doi.org/10.5380/rber.v6i5.52601.

Ayadi, I., Belghith, H., Gargouri, A., & Guerfali, M. (2018). Screening of new oleaginous yeasts for single cell oil production, hydrolytic potential exploitation and agro-industrial by-products valorization. Process Safety and Environmental Protection, 119, 104–114. https://doi.org/10.1016/j.psep.2018.07.012.

Bansal, N., Dasgupta, D., Hazra, S., Bhaskar, T., Ray, A., & Ghosh, D. (2020). Effect of utilization of crude glycerol as substrate on fatty acid composition of an oleaginous yeast Rhodotorula mucilagenosa IIPL32: Assessment of nutritional indices. Bioresource Technology, 309, 123330. https://doi.org/10.1016/j.biortech.2020.123330.

Bettencourt, S. Miranda, C., Pozdniakova, T. A., Sampaio, P., Franco-Duarte, R., & Pais, C. (2020). Single cell oil production by oleaginous yeasts grown in synthetic and waste-derived volatile fatty acids. Microorganisms, 8(11), 1–18. https://doi.org/10.3390/microorganisms8111809.

Cao, X., Pan, Y., Wei, W., Yuan, T., Wang, S., Xiang, L., & Yuan, Y. (2021). Single cell oil production by Trichosporon sp.: Effects of fermentation conditions on fatty acid composition and applications in synthesis of structured triacylglycerols. Lwt, 148, 111691. https://doi.org/10.1016/j.lwt.2021.111691.

Cardoso, B. F., Shikida, F. A., & Finco, A. (2017). Análise Fatorial do Sistema Agroindustrial do Biodiesel no Brasil e na União Europeia. Revista de Economia e Sociologia Rural, 55(3), 551–568. https://doi.org/10.1590/1234-56781806-94790550308.

Carota, E., Petruccioli, M., D’annible, A., & Crognale, S. (2020). Mixed glycerol and orange peel-based substrate for fed-batch microbial biodiesel production. Heliyon, 6(9), e04801. https://doi.org/10.1016/j.heliyon.2020.e04801.

Cintra, J. S. A., Portela, M. N., Silvany, T. C. de C., Pedroza, G. A. G., Santos, L. C. L. dos, & Lobato, A. K. de C. L. (2017). Influência do tempo de reação na produção de biodiesel via catálise heterogênea. Holos, 1, 195-204. https://doi.org/10.15628/holos.2017.5197.

Cozendey, D. A., Muniz, R. O., Dos Santos, R. C., De Souza, C. G., De Andrade, D. F., & D’Avila, L. A. (2021). Quantitative analysis of free glycerol in biodiesel using solidphase extraction and high-performance liquid chromatography. Microchemical Journal, 168. https://doi.org/10.1016/j.microc.2021.106347.

Da Silva, M. P. D., Glória, J. J., Panta, D. A. S., & Vieira, G. E. G. (2021). Microalgas e a terceira geração de biocombustíveis: desafios atuais e perspectivas futuras. Revista Desafios, 8, 58–76, 2021. http://dx.doi.org/10.20873/uftv8-11171.

Di Fidio, N. Minonne, F., Antonetti, C., & Galletti, A. M. R. (2021). Cutaneotrichosporon oleaginosus: A versatile whole-cell biocatalyst for the production of single-cell oil from agro-industrial wastes. Catalysts, 11(11), 1291. https://doi.org/10.3390/catal11111291.

Diwan, B., Parkhey, P., & Gupta, P. (2018). From agro-industrial wastes to single cell oils: a step towards prospective biorefinery. Folia Microbiologica, 63(5), 547– 568. https://doi.org/10.1007/s12223-018-0602-7.

Dobrowolski, A., Mitula. P., Rymowicz, W., & Mironczuk, A. M. (2016). Efficient conversion of crude glycerol from various industrial wastes into single cell oil by yeast Yarrowia lipolytica. Bioresource Technology, 207, 237–243. https://doi.org/10.1016/j.biortech.2016.02.039.

Gajdoš, P., Nicaud, J. M., & Čertík, M. (2017). Glycerol conversion into a single cell oil by engineered Yarrowia lipolytica. Engineering in Life Sciences, 17(3), 325– 332. https://doi.org/10.1002/elsc.201600065.

Garlapati, V. K., Shankar, U., & Budhiraja, A. (2016). Bioconversion technologies of crude glycerol to value added industrial products. Biotechnology Reports, 9, 9–14. https://doi.org/10.1016/j.btre.2015.11.002.

Guerfali, M., Ayadi, I., Belhassen, A., Gargouri, A., & Belghith, H. (2018). Single cell oil production by Trichosporon cutaneum and lignocellulosic residues bioconversion for biodiesel synthesis. Process Safety and Environmental Protection, 113, 292–304. https://doi.org/10.1016/j.psep.2017.11.002.

Guerfali, M., Ayadi, I., Sassi, H-E., Belhassen, A., Gargouri, A., & Belghith, H. (2020). Biodiesel-derived crude glycerol as alternative feedstock for single cell oil production by the oleaginous yeast Candida viswanathii Y-E4. Industrial Crops and Products, 145:112103. https://doi.org/10.1016/j.indcrop.2020.112103.

Hyppolito, M. L. De Souza, L. A., Da Silva, J. I., Da Silva, F. C., & Lopes, F. L. (2021). Production and characterization of diesel mixtures with corn oil biodiesel. Revista em Agronegocio e Meio Ambiente, 14(4). https://doi.org/10.17765/2176-9168.2021v14n4e8872.

Kamoun, O., Ayadi, I., Guerfali, M., Belghith, H., Gargouri, A., & Trigui-Lahiani, H. (2018). Fusarium verticillioides as a single-cell oil source for biodiesel production and dietary supplements. Process Safety and Environmental Protection, 118, 68–78. https://doi.org/10.1016/j.psep.2018.06.027.

Karamerou, E. E., Theodoropoulos, C., & Webb, C. A. (2016). Biorefinery approach to microbial oil production from glycerol by Rhodotorula glutinis. Biomass and Bioenergy, 89, 113–122. https://doi.org/10.1016/j.biombioe.2016.01.007.

Kolouchová, I., Mat’átková, O., Sigler, K., Masák, J., & Rezanka, T. (2016). Lipid accumulation by oleaginous and non-oleaginous yeast strains in nitrogen and phosphate limitation. Folia Microbiol, 61(5):431–438. https://doi.org/10.1007/s12223-016-0454-y.

Kumar, L. R., Kaur, R., Tyagi, R. D., & Drogui, P. (2021). Identifying economical route for crude glycerol valorization: Biodiesel versus polyhydroxy-butyrate (PHB). Bioresource Technology, 323, 124565. https://doi.org/10.1016/j.biortech.2020.124565.

Lopes, H. J. S., Bonturi, N., & Miranda, E. A. (2020). Rhodotorula toruloides single cell oil production using Eucalyptus urograndis hemicellulose hydrolysate as a carbon source. Energies, 13(4), 795. https://doi.org/10.3390/en13040795.

Ma, X., Liu, F., Helian, Y., Li, C., Wu, Z., Li, H., & Zhou, S. (2021). Current application of MOFs based heterogeneous catalysts in catalyzing transesterification/esterification for biodiesel production: A review. Energy Conversion and Management, 229, 113760. https://doi.org/10.1016/j.enconman.2020.113760.

Madani, M., Enshaeieh, M., & Abdoli, A. (2017). Single cell oil and its application for biodiesel production. Process Safety and Environmental Protection, 111, 747–756. https://doi.org/10.1016/j.psep.2017.08.027.

Medeiros, J. F. Berni, J. V., Diório, A., Saraiva, A. C. B., Gomes, M. C. S., & Pereira, N. C. (2019). Remoção de cor da glicerina bruta por adsorção em carvão ativado vegetal. Revista Brasileira de Energias Renováveis, 8(2). http://dx.doi.org/10.5380/rber.v8i2.65658.

Medina, J. D. C. Magalhães Junior, A. I., Zamora, H. D., & Melo, J. D. Q. (2019). Oil palm cultivation and production in South America: status and perspectives. Biofuels, Bioproducts and Biorefining, 13(5), 1202– 1210. https://doi.org/10.1002/bbb.2013.

Mhlongo, S. I. Ezeokoli, O. T., Roopnarain, A., Ndaba, B., Sekoai, P. T., Habimana, O., & Pohl, C. H. (2021). The potential of single-cell oils derived from filamentous fungi as alternative feedstock sources for biodiesel production. Frontiers in Microbiology, 12. https://doi.org/10.3389/fmicb.2021.637381.

Oliveira, M. Ramos, A., Monteiro, E., & Rouboa, A. (2022). Improvement of the Crude Glycerol Purification Process Derived from Biodiesel Production Waste Sources through Computational Modeling. Sustainability (Switzerland), 14(3). https://doi.org/10.3390/su14031747.

Papanikolaou, S., Rontou, M., Belka, A., Athenaki, M., Gardeli, C., Mallouchos, A., & Aggelis, G. (2017). Conversion of biodiesel-derived glycerol into biotechnological products of industrial significance by yeast and fungal strains. Engineering in Life Sciences, 17, 262–281. https://doi.org/10.1002/elsc.201500191.

Pardal, A., Fernandes, M. C., Éncinar, J, M., Sanches, N., & Chaves, H. (2017). Utilização do biodiesel: perspectiva química e ambiental. Research and Networks in Health, 1(3), 6.

Parsons, S., Allen, M. J., & Chuck, C. J. (2020). Coproducts of algae and yeast-derived single cell oils: A critical review of their role in improving biorefinery sustainability. Bioresource Technology, 303, 122862. https://doi.org/10.1016/j.biortech.2020.122862.

Qin, L. Liu, L., Zeng, A-P., & Wei, D. (2017). From low-cost substrates to Single Cell Oils synthesized by oleaginous yeasts. Bioresource Technology, 245, 1507–1519. https://doi.org/10.1016/j.biortech.2017.05.163.

Ram, S. K., Tyagi, R. D., & Drogui, P. (2018). Effect of sludge concentration and crude glycerol matrix as a substrate on the production of single-cell oil by oleaginous yeast Yarrowia lipolytica SKY7. Fermentation, 4(2). https://doi.org/10.3390/fermentation4020024.

Ramos, L. P., Kothe, V., César-Oliveira. M. A. F., Muniz-Wypych, A. S., Nakagaki, S., Krieger, N., Wypych, F., & Cordeiro, C. S. (2017). Biodiesel: Raw materials, production technologies and fuel properties. Revista Virtual de Química, 9(1), 317–369. http://dx.doi.org/10.21577/1984-6835.20170020.

Rezende, D. B., & Pasa, V. M. D. (2017). Tendências e oportunidades para pesquisas em biocombustíveis. The Journal of Engineering and Exact Sciences - JCEC, 03, 561–572. https://doi.org/10.18540/2446941602012016001.

Sagia, S., Sharma, A., Singh, S., Chaturvedi, S., Nain, P. K. S., & Nain, L. (2020). Single cell oil production by a novel yeast Trichosporon mycotoxinivorans for complete and ecofriendly valorization of paddy straw. Electronic Journal of Biotechnology, 44, 60–68. https://doi.org/10.1016/j.ejbt.2020.01.009.

Schirmer, W. N., & Ribeiro, C. B. (2017). Panorama dos combustíveis e biocombustíveis no brasil e as emissões gasosas decorrentes do uso da gasolina/etanol. BIOFIX Scientific Journal, 2(2), 16-22. dx.doi.org/10.5380/biofix.v2i2.53539.

Singh, D., Sharma, D., Soni, S. L., Sharma, S., Sharma, K. P., & Jhalani, A. (2020). Review article A review on feedstocks, production processes, and yield for different generations of biodiesel. Fuel, 262, 116553. https://doi.org/10.1016/j.fuel.2019.116553.

Uprety, B. K., Dalli, S. S., & Rakshit, S. K. (2017). Bioconversion of crude glycerol to microbial lipid using a robust oleaginous yeast Rhodosporidium toruloides ATCC 10788 capable of growing in the presence of impurities. Energy Conversion and Management, 135, 117–128. https://doi.org/10.1016/j.enconman.2016.12.071.

Varão, L. H. R., Silva, T. A. L., Zamora, H. D. Z., & Pasquini, D. (2017). Vantagens e limitações do sebo bovino enquanto matéria prima para a indústria brasileira de biodiesel. Holos, 7, 39–54. https://doi.org/10.15628/holos.2017.5010

Viñarta, S. C., Angelicola, M. V., Barros, M., Fernandez, P. M., Cormal, W. M., Aybar, M. J., & Figueroa, L. I. C. (2016). Oleaginous yeasts from Antarctica: Screening and preliminary approach on lipid accumulation. Journal of Basic Microbiology, 56(12), 1360–1368. https://doi.org/10.1002/jobm.201600099.

Vuono, F. M. (2021). Usabilidade de ícones em Ambientes Virtuais de Aprendizagem: uma análise pela ótica da neurociência e da experiência do usuário. Tese, Programa de Pós-graduação em Design - Universidade do Estado do Rio de Janeiro, Rio de Janeiro.

Wang, G., Bai, T., Miao, Z., Ning, W., & Liang, W. (2018). Simultaneous production of single cell oil and fumaric acid by a newly isolated yeast Aureobasidium pullulans var. aubasidani DH177. Bioprocess and Biosystems Engineering, 41(11), 1707–1716. https://doi.org/10.1007/s00449-018-1994-0.

Yoshinaga, F., Santos, A. S., Moura, B. F. S., & Bortoleto, G. G. (2020). Bioquerosene para aviação: cenário atual e perspectivas futuras. Bioeneria em Revista: Diálogos,10(1), 73–91.

Zuccaro, G., Del Mondo, A., Pinto, G., Pollio, A., & De Natale, A. (2021). Biorefinery-based approach to exploit mixed cultures of Lipomyces starkeyi and Chloroidium saccharophilum for single cell oil production. Energies, 14(5), 1340. https://doi.org/10.3390/en14051340.

Published

09/12/2022

How to Cite

CARDOSO, R. A. A. N. .; DAQUILA, B. V. .; CONTE, H.; OLIVEIRA, J. A. dos S. . Synthesis of single cell oil from crude glycerol: a systematic literature review. Research, Society and Development, [S. l.], v. 11, n. 16, p. e316111638071, 2022. DOI: 10.33448/rsd-v11i16.38071. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/38071. Acesso em: 7 may. 2024.

Issue

Section

Review Article