Optimization process of vaccine production of outer membrane vesicle from Neisseria meningitidis associated with Zika virus
Keywords:OMV; Zika Virus; Vaccine; Outer membrane vesicle; Scheduling.
Several vaccine prototypes were made using variations in concentration, volume, time and speed of agitation. From these prototypes, some characterization assays were carried out, such as the analysis of particle size and zeta potential, where it was possible to determine the size of the nanoparticles, the polydispersity index, with the most promising samples having a size of 252.1nm ± 71.2 nm. The polydispersity index was 0.572 ±0.02, the standard deviation being a little high due to the presence of free viral particles and some associated vesicles. It is noteworthy that the isolated vesicle has a size of 140 to 160nm and the polydispersity index of 0.513 demonstrates that we do not have interference in the sample of our vesicle (it is known that the size of the viral particle of Zika virus is between 40 and 60nm). Cytotoxicity testing was done to analyze whether they have cytotoxic profiles and also demonstrate that the prototype's neutralization and inactivation method works correctly. The analysis of the protein profile of some of the samples revealed that there are two major Zika virus proteins in the fused proteasome, namely the E protein, which is directly involved in the recognition of host cell receptors, and the C protein, which is related to capsid assembly, in addition to playing an important role in the immune response.
Agumadu, V. C., & Ramphul, K. (2018). Zika Virus: A Review of Literature. Cureus, 10(7), e3025. https://doi.org/10.7759/cureus.3025
Alves, D.A., Mattos, I.B., Hollanda, L.M., & Lancellotti, M. (2013). Use of Mesoporous Silica SBa-15 and SBa-16 in Association of Outer Membrane Vesicles - OMV from Neisseria meningitidis. Journal of Vaccines and Vaccination, 2013, 1-6.
Amaral P., Resende de Carvalho L., Hernandes Rocha T.A., da Silva N.C. & Vissoci J.R.N. (2019) Geospatial modeling of microcephaly and zika virus spread patterns in Brazil. PLOS ONE 14(9): e0222668.
Barrett, A.D.T. (2018) Current status of Zika vaccine development: Zika vaccines advance into clinical evaluation. npj Vaccines 3, 24.
Barreto-Vieira, D. F., Barth, O. M., Silva, M. A. N. da., Santos, C. C., Santos, A. da S., F Filho, J. B., & Filippis, A. M. B. de. (2016). Ultrastructure of Zika virus particles in cell cultures. Memórias Do Instituto Oswaldo Cruz, 111(Mem. Inst. Oswaldo Cruz, 2016 111(8)), 532–534. https://doi.org/10.1590/0074-02760160104
Chen, Y., Chi, X., Zhang, H., Zhang, Y., Qiao, L., Ding, J., Han, Y., Linm Y. & Jiang, J. (2023). Identification of Potent Zika Virus NS5 RNA-Dependent RNA Polymerase Inhibitors Combining Virtual Screening and Biological Assays. International Journal of Molecular Sciences, 24(3), 1900. MDPI AG. Retrieved from http://dx.doi.org/10.3390/ijms24031900
Cruz-Oliveira, C., Freire, J. M., Conceição, T. M., Higa, L. M., Castanho, M. A., & Da Poian, A. T. (2015). Receptors and routes of dengue virus entry into the host cells. FEMS microbiology reviews, 39(2), 155–170. https://doi.org/10.1093/femsre/fuu004
Dai, L., Song, J., Lu, X., Deng, Y. Q., Musyoki, A. M., Cheng, H., Zhang, Y., Yuan, Y., Song, H., Haywood, J., Xiao, H., Yan, J., Shi, Y., Qin, C. F., Qi, J., & Gao, G. F. (2016). Structures of the Zika Virus Envelope Protein and Its Complex with a Flavivirus Broadly Protective Antibody. Cell host & microbe, 19(5), 696–704. https://doi.org/10.1016/j.chom.2016.04.013
Garcez, P. P., Loiola, E. C., Madeiro da Costa, R., Higa, L. M., Trindade, P., Delvecchio, R., Nascimento, J. M., Brindeiro, R., Tanuri, A., & Rehen, S. K. (2016). Zika virus impairs growth in human neurospheres and brain organoids. Science (New York, N.Y.), 352(6287), 816–818. https://doi.org/10.1126/science.aaf6116
Giraldo, M. I., Gonzalez-Orozco, M., & Rajsbaum, R. (2023). Pathogenesis of Zika Virus Infection. Annual review of pathology, 18, 181–203. https://doi.org/10.1146/annurev-pathmechdis-031521-034739
Gurumayum, S., Brahma, R., Naorem, L. D., Muthaiyan, M., Gopal, J., & Venkatesan, A. (2018). ZikaBase: An integrated ZIKV- Human Interactome Map database. Virology, 514, 203–210. https://doi.org/10.1016/j.virol.2017.11.007
Labib, B. A., & Chigbu, D. I. (2022). Pathogenesis and Manifestations of Zika Virus-Associated Ocular Diseases. Tropical Medicine and Infectious Disease, 7(6), 106. MDPI AG. Retrieved from http://dx.doi.org/10.3390/tropicalmed7060106
Lee, J., & Shin, O. (2019). Advances in Zika Virus–Host Cell Interaction: Current Knowledge and Future Perspectives. International Journal of Molecular Sciences, 20(5), 1101. MDPI AG. Retrieved from http://dx.doi.org/10.3390/ijms20051101
Markoff, L. (2003) 5′-and 3′-noncoding regions in Flavivirus RNA. ISSN 0065-3527
Martins, P., Machado, D., Theizen, T. H., Guarnieri, J. P. O., Bernardes, B. G., Gomide, G. P., Corat, M. A. F., Abbehausen, C., Módena, J. L. P., Melo, C. F. O. R., Morishita, K. N., Catharino, R. R., Arns, C. W., & Lancellotti, M. (2018). Outer Membrane Vesicles from Neisseria Meningitidis (Proteossome) Used for Nanostructured Zika Virus Vaccine Production. Scientific reports, 8(1), 8290. https://doi.org/10.1038/s41598-018-26508-z
Pang, Z., Chong, J., Zhou, G., Morais D., Chang, L., Barrette, M., Gauthier, C., Jacques, PE., Li, S., and Xia, J. (2021) MetaboAnalyst 5.0: narrowing the gap between raw spectra and functional insights Nucl. Acids Res. (doi: 10.1093/nar/gkab382)
Pattnaik, A., Sahoo, B. R., & Pattnaik, A. K. (2020). Current Status of Zika Virus Vaccines: Successes and Challenges. Vaccines, 8(2), 266.
Roby, J. A., Setoh, Y. X., Hall, R. A., & Khromykh, A. A. (2015). Post-translational regulation and modifications of flavivirus structural proteins. The Journal of general virology, 96(Pt 7), 1551–1569. https://doi.org/10.1099/vir.0.000097
van der Pol, L., Stork, M., & van der Ley, P. (2015). Outer membrane vesicles as platform vaccine technology. Biotechnology journal, 10(11), 1689–1706. https://doi.org/10.1002/biot.201400395
World Health Organization. The history of zika virus (2016). https://www.who.int/news-room/feature-stories/detail/the-history-of-zika-virus
Yeasmin M., Molla M.M.A., Masud H.M.A.A., Saif-Ur-Rahman K.M. (2023) Safety and immunogenicity of Zika virus vaccine: a systematic review of clinical trials. Rev Med Virol; 33(1): e2385. https://doi.org/10.1002/rmv.2385
Zhang, Y., Corver, J., Chipman, P. R., Zhang, W., Pletnev, S. V., Sedlak, D., Baker, T. S., Strauss, J. H., Kuhn, R. J., & Rossmann, M. G. (2003). Structures of immature flavivirus particles. The EMBO journal, 22(11), 2604–2613. https://doi.org/10.1093/emboj/cdg270
How to Cite
Copyright (c) 2023 João Paulo de Oliveira Guarnieri; Bruno Gaia Bernardes; Carlos Fernando Macedo da Silva; Janaína Artem Ataide; Arthur Noin de Oliveira; Jeany Delafiori; Rodrigo Ramos Catharino; Priscila Gava Mazzola; Marcelo Lacellotti
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.