Comparison of four serological tests for the diagnosis of swine brucellosis

Authors

DOI:

https://doi.org/10.33448/rsd-v9i7.4418

Keywords:

Fluorescence polarization; Complement fixation; Rose Bengal; Tube agglutination; Brucella suis.

Abstract

The purpose of this article is to present results of a research in which the results of four serological tests were compared for the diagnosis of swine brucellosis in pigs from Brazilian herds in which the outbreak occurred in relation to other brucellosis-free.  Rose Bengal (RBT), complement fixation (CFT), agglutination plus 2-mercaptoethanol test (TAT+ME), and commercial fluorescence polarization assay (FPA) were used to test 333 sera (271 sows and 62 finishing pigs) from a Brucella suis infected swine herd and 1,100 swine sera from brucellosis free pigs taken at a slaughterhouse. Considering infected all the 271 sows from the outbreak, and interpreting results of the FPA according to the manufacturer directions, sensitivities observed were 95.94% for FPA, 94.83% for RBT, 93.73% for CFT, and 92.25% for TAT+ME. Considering infected all the pigs from the infected herd with a positive result in at least one test, sensitivities observed were 98.92% for RBT, 97.13% for CFT, 96.06% for FPA, and 94.98% for TAT+ME. Specificities of the tests were CFT and TAT+ME, 100%; FPA, 99.55%; and RBT 99.27%. Results indicated a good performance of all the tests, and the agreement among them was almost perfect.

Author Biography

Raphaella Barbosa Meirelles-Bartoli, Universidade Federal de Jataí

Mestre e Doutora em Medicina Veterinária Preventiva pela Unesp de Jaboticabal-SP. Docente desde 2011 na Universidade Federal de Jataí (UFJ) atuando na área de Doenças Infecciosas, Epidemiologia, Saúde Pública e Saúde Única. Responsável por disciplinas na graduação do curso de Medicina Veterinária, na Residência Multiprofissional em Saúde - Medicina Veterinária e no Programa de Pós Graduação em Biociência Animal. Coordenadora do Laboratório de Saúde Pública Veterinária e do Núcleo de Imunização e Sorologia Antirrábica Humana e Animal na UFJ. Coodenadora de Estágios do curso de Medicina Veterinária. Coordenadora do Grupo de Estudos em Saúde Pùblica Veterinária. Membro da Comissão de Saúde Animal da AUGM. Membro da Comissão Estadual de Saúde Pública do CRMV-GO.

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Published

23/05/2020

How to Cite

MEIRELLES-BARTOLI, R. B.; CRUZ, C. de A.; MORAES, R. S. de; OLIVEIRA, R. A.; PAULA, E. M. N. de; SOUSA, D. B. de; ASSIS, N. A. de; MATHIAS, L. A. Comparison of four serological tests for the diagnosis of swine brucellosis. Research, Society and Development, [S. l.], v. 9, n. 7, p. e493974418, 2020. DOI: 10.33448/rsd-v9i7.4418. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/4418. Acesso em: 18 apr. 2024.

Issue

Vol. 9 No. 7

Section

Agrarian and Biological Sciences

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