Comparison of four serological tests for the diagnosis of swine brucellosis

Authors

DOI:

https://doi.org/10.33448/rsd-v9i7.4418

Keywords:

Fluorescence polarization; Complement fixation; Rose Bengal; Tube agglutination; Brucella suis.

Abstract

The purpose of this article is to present results of a research in which the results of four serological tests were compared for the diagnosis of swine brucellosis in pigs from Brazilian herds in which the outbreak occurred in relation to other brucellosis-free.  Rose Bengal (RBT), complement fixation (CFT), agglutination plus 2-mercaptoethanol test (TAT+ME), and commercial fluorescence polarization assay (FPA) were used to test 333 sera (271 sows and 62 finishing pigs) from a Brucella suis infected swine herd and 1,100 swine sera from brucellosis free pigs taken at a slaughterhouse. Considering infected all the 271 sows from the outbreak, and interpreting results of the FPA according to the manufacturer directions, sensitivities observed were 95.94% for FPA, 94.83% for RBT, 93.73% for CFT, and 92.25% for TAT+ME. Considering infected all the pigs from the infected herd with a positive result in at least one test, sensitivities observed were 98.92% for RBT, 97.13% for CFT, 96.06% for FPA, and 94.98% for TAT+ME. Specificities of the tests were CFT and TAT+ME, 100%; FPA, 99.55%; and RBT 99.27%. Results indicated a good performance of all the tests, and the agreement among them was almost perfect.

Author Biography

Raphaella Barbosa Meirelles-Bartoli, Universidade Federal de Jataí

Mestre e Doutora em Medicina Veterinária Preventiva pela Unesp de Jaboticabal-SP. Docente desde 2011 na Universidade Federal de Jataí (UFJ) atuando na área de Doenças Infecciosas, Epidemiologia, Saúde Pública e Saúde Única. Responsável por disciplinas na graduação do curso de Medicina Veterinária, na Residência Multiprofissional em Saúde - Medicina Veterinária e no Programa de Pós Graduação em Biociência Animal. Coordenadora do Laboratório de Saúde Pública Veterinária e do Núcleo de Imunização e Sorologia Antirrábica Humana e Animal na UFJ. Coodenadora de Estágios do curso de Medicina Veterinária. Coordenadora do Grupo de Estudos em Saúde Pùblica Veterinária. Membro da Comissão de Saúde Animal da AUGM. Membro da Comissão Estadual de Saúde Pública do CRMV-GO.

References

Alton, GG, Jones, LM, Angus, RD & Verger, JM. (1988). Techniques for the brucellosis laboratory. Institut Nacional de la Recherche Agronomique.

Brasil. (2017). Instrução normativa nº 10, de 3 de março de 2017. Ministério da Agricultura, Pecuária e Abastecimento. http://www.in.gov.br/materia/-/asset_publisher/Kujrw0TZC2Mb/content/id/19124587/do1-2017%E2%80%9306-20-instrucao-normativa-n-10-de-3-de-marco-de-2017%E2%80%9319124353

Di Febo, T, Luciani, M, Portanti, O, Bonfini, B, Lelli, R & Tittarelli, M. (2012). Development and evaluation of diagnostic tests for the serological diagnosis of brucellosis in swine. Veterinaria Italiana, 48 (2), 145-56. http://www.izs.it/vet_italiana/2012/48_2/145.pdf

Dohoo, I, Martin, W & Stryhn, H. (2009). Veterinary epidemiologic research. Ver Inc.

Jacobson, RH. (1998). Validation of serological assays for diagnosis of infectious diseases. Revue Scientifique et Technique, 17 (2), 469-86. https://doi.org/10.20506/rst.17.2.1119

Landis, RR & Kock, GG. (1977). The measurement of observer agreement for categorical data. Biometrics, 33 (1), 159-74. https://doi.org/10.2307/2529310

MacMillan, AP. (1999). Brucellosis. In: Straw, BE, D´Allaire, S, Mengeling, WL & Taylor, DM (Eds.), Diseases of swine (pp. 385-39). Blackwell Publishing.

Meirelles-Bartoli, RB, Mathias, LA & Samartino, LE. (2012). Brucellosis due to Brucella suis in a swine herd associated with a human clinical case in the State of São Paulo, Brazil. Tropical Animal Health and Production, 44 (7), 1575-1579. https://doi.org/10.1007/s11250-012-0108-2

Musser, JMB, Schwartz, AL, Srinath, I & Waldrup, KA. (2013). Use of serology and bacterial culture to determine prevalence of Brucella spp. in feral swine (Sus scrofa) in proximity to a beef cattle herd positive for Brucella suis and Brucella abortus. Journal of Wildlife Diseases, 49, 215-220. https://doi.org/10.7589/2012-06-169

Nielsen, K, Gall, D, Jolley, M, Leishman, G, Balsevicius, S, Smith, P, Nicoletti, P & Thomas, F. (1996). A homogeneous fluorescence polarization assay for detection of antibody to Brucella abortus. Journal of Immunological Methods, 195 (1-2), 161-168. https://doi.org/10.1016/0022-1759(96)00116-0

Nielsen, K., Gall, D., Smith, P., Vigliocco, A., Perez, B., Samartino, L., Nicoletti, P., Dajer, A., Elzer, P., & Enright, F. (1999). Validation of the fluorescence polarization assay as a serological test for the presumptive diagnosis of porcine brucellosis. Veterinary Microbiology, 68 (3-4), 245-253. https://doi.org/10.1016/s0378-1135(99)00077-2

Pereira, AS, Shitsuka, DM, Parreira, FJ & Shitsuka, R. (2018). Metodologia da pesquisa científica. [e-book]. Santa Maria. Ed. UAB/NTE/UFSM. Disponível em: https://repositorio.ufsm.br/bitstream/handle/1/15824/Lic_Computacao_Metodologia-Pesquisa-Cientifica.pdf?sequence=1.

Praud, A., Gimenez, O., Zanella, G., Dufour, B., Pozzi, N., Antras, V., Meyer, L., & Garin-Bastuji, B. (2012). Estimation of sensitivity and specificity of five serological tests for the diagnosis of porcine brucellosis. Preventive Veterinary Medicine, 104 (1-2), 94-100. https://doi.org/10.1016/j.prevetmed.2011.10.014

Silva Paulo, P., Vigliocco, A. M., Ramondino, R. F., Marticorena, D., Bissi, E., Briones, G., Gorchs, C., Gall, D., & Nielsen, K., (2000). Evaluation of primary binding assays for presumptive serodiagnosis of swine brucellosis in Argentina. Clinical and Diagnostic Laboratory Immunology, 7 (5), 828-831. https://dx.doi.org/10.1128%2Fcdli.7.5.828-831.2000

R Core Team. (2013). R: A language and environment for statistical computing. http://www.R-project.org/

Stoffregen, W. C., Olsen, S. C., Wheeler, C. J., Bricker, B. J., Palmer, M. V., Jensen, A. E., Halling, S. M., & Alt, D. P. (2007). Diagnostic characterization of a feral swine herd enzootically infected with Brucella. Journal of Veterinary Diagnostic Investigation, 19, 227-237. https://doi.org/10.1177%2F104063870701900301

Thrusfield, M. (2005). Veterinary epidemiology. Blackwell Publishing.

World Organization for Animal Health (2009). Manual of diagnostic tests and vaccines for terrestrial animals. OIE.

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Published

23/05/2020

How to Cite

MEIRELLES-BARTOLI, R. B.; CRUZ, C. de A.; MORAES, R. S. de; OLIVEIRA, R. A.; PAULA, E. M. N. de; SOUSA, D. B. de; ASSIS, N. A. de; MATHIAS, L. A. Comparison of four serological tests for the diagnosis of swine brucellosis. Research, Society and Development, [S. l.], v. 9, n. 7, p. e493974418, 2020. DOI: 10.33448/rsd-v9i7.4418. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/4418. Acesso em: 23 nov. 2024.

Issue

Vol. 9 No. 7

Section

Agrarian and Biological Sciences

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