Onion (Allium cepa) DNA extraction: Perspective of scientific practices through laboratory practice
DOI:
https://doi.org/10.33448/rsd-v12i14.44583Keywords:
Deoxyribonucleic acid; Laboratory practice; DNA extraction; High school.Abstract
DNA represents the structural unit of life, being one of the best-known organic substances. DNA extraction has become a alternative to teaching-learning in Biology classes. In this sense, the objective of this work was to facilitate the learning of high school Biology students, based on an experimental activity involving the extraction of DNA from onions (Allium cepa). The activity was implemented in a practical class laboratory at IFPE-Campus Barreiros in two pedagogical moments. The first moment consisted of a practical class for extracting DNA from A. cepa, using everyday materials. In the second moment, students were invited to evaluate the activity through a questionnaire. From the analysis of the results, it was possible to verify that the proposed activity encouraged motivation, aroused curiosity and facilitated students learning. Furthermore, the evaluation of the activity by the students was considered stimulating by the authors. Finally, in addition to combining theory with practice, this work can be useful as reference material on the DNA molecule.
References
Araújo, M. S., & Freitas, W. L. S. (2019). A experimentação no ensino de biologia: uma correlação entre teoria e prática para alunos do ensino médio em Floriano/PI. Revista de Ensino de Biologia da SBEnBio, 12 (1): 22-35.
Brasil (1996). Lei de Diretrizes e Bases da Educação Nacional, LDB. 9394/1996.
Brasil (2017a). Base Nacional Comum Curricular (BNCC). MEC.
Brasil (2017b). Parâmetros Curriculares Nacionais para o Ensino Médio (PCNEM). MEC.
Cardoso, T.C., Lima, M. M. O., Araújo, J. S., Alves, W. S., Martins, F. A., & Almeida, P. M. (2021). Molecular and Forensic Biology in High School. Research, Society and Development, 10 (8), e47710817624.
Demir, K. (2016). “Uncooking” a Traditional DNA-Extraction Laboratory from the Scientific-Practices Perspective. The American Biology Teacher, 78 (7): 582-590.
De Robertis, E. M. F., HIB, J. (2014). Bases da biologia celular e molecular. 16. ed., Rio de Janeiro: Guanabara Koogan.
Fagundes, S. F., Nascimento, C. N., & Brito, D. Q., (2022). A prática experimental: extração de DNA aplicada ao Ensino Básico. Physicae Organum, 8 (1): 233-248.
Furlan, C. M., Almeira, A. C., Rodrigues, C. D. N., Tanigushi, D. G., Santos, D. Y. A. C., Motta, L. B. & Chow, F. (2011). Extração de DNA vegetal: o que estamos realmente ensinando em sala de aula? Química Nova na Escola, 33(1).
Gonçalves, T. M. (2021). Extraindo o DNA de vegetais: uma proposta de aula prática para facilitar a aprendizagem de Genética no Ensino Médio. Revista Educação Pública, 21 (15).
Gonçalves, T. M. (2022). Genetics in the kitchen: an experimental activity in the home extraction of DNA from Kiwi fruit (Actinidia deliciosa). Research, Society and Development, 11 (4): 1-10.
Hearn, R.P., & Arblaster, K.E. (2010). DNA Extraction techniques for use in education. Biochemistry and Molecular Biology Education, 38 (3): 161-166.
Kinoshita, Y., Yamanoi, T., & Takemura, M. (2016). Extracting DNA to visualize the unity & diversity of life. The American Biology Teacher, 78 (2): 118-126.
Matta, L. D. M., Santos, I. R., Mendonça, S. C. S., Carvalho, D. V. M., Silveira, A. P. M., & Silva, R. P. (2020). Ensino e aprendizagem de biomoléculas no ensino médio: extração de DNA e estímulo à experimentação. Revista de Ensino de Biologia da SBEnBio, 13 (1): 59-73.
Sepel, L. M. N., & Loreto, E. L. S. (2002). Isolation and visualization of nucleic acid with homemade apparatus. Biochemistry and Molecular Biology Education, 30, 5: 306–308.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Edmilson Clarindo de Siqueira; Bogdan Doboszewski
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
