Cassava hydrolysates used as substrates for the production of second-generation ethanol by Saccharomyces cerevisiae ATCC 26602 immobilized in sodium alginate spheres




Fermentation; Yeast; Celullosic ethanol; Acid hydrolysis; Residue.


Cassava has a prominent position in the Brazilian agriculture and economy. During the production of flour, a very large volume of residue is generated. One way to use these residues is through the production of ethanol. In this context, the present research aimed to use cassava residues as raw material for ethanol production. For this, sulfuric acid was used at concentrations of 1.0 to 5.0% during 5, 10 and 15 minutes of heating in an autoclave. The fermentation was carried out by Saccharomyces cerevisiae ATCC 26602, and to achieve a better performance, it was immobilized in sodium alginate spheres. Ethanol production was also estimated using a synthetic medium added with glucose that served as a comparison standard. The results of the experiment showed that the highest concentration of reducing sugars obtained by the hydrolysis of the residue was with the use of 2.0% of H2SO4 with 15 min. heating at 121 ºC, releasing 56.26 g/L of reducing sugars. The parameters used that led to the highest production of ethanol by the yeast, 7.85 g/L of ethanol, were: pH of 6.5, growth temperature of 30 °C, without agitation. The results showed that cassava residues can be used as a potential substrate for ethanol production. Thus, this work presents data on the most suitable conditions for the use of these industrial wastes in order to generate less polluting fuel energy, an increasingly attractive feature in a world where economic and environmental concerns grow every day.


Behera, S., Mohanty, R. C. & Ray, R. C. (2010). Comparative study of bio-ethanol production from mahula (Madhuca latifolia L.) flowers by Saccharomyces cerevisiae and Zymomonas mobilis. Applied Energy. 87(1), 2352-2355.

Chaovanalikit, A. & Wrolstad, R. (2004). Total Anthocyanins and Total Phenolics of Fresh and Processed Cherries and Their Antioxidant Properties. Journal of Food Science. 69(1), FCT67- FCT72.

Correia, I. A., Scandelai, A. P. J., Silva, V. A., Nadine C. B. & Souza, A. T. de S. (2018). Caracterização da manipueira e possibilidades de tratamento. Colloquium Exactarum. 10, 180-185.

Dubois, M. et al. (1956). Colorimetric method for determination of sugars and related substances. Anal. Chem. 28(1), 350-356.

Hashem, M. & Darwish, S. (2010). Production of bioethanol and associated by-products from potato starch residue stream by Saccharomyces cerevisiae. Biomass and Bioenergy. 34(1), 953-959.

Larsson, S. et al. (1999b). The generation of fermentation inhibitors during dilute acid hydrolysis of softwood. Enz. Microb. Technol. 24(3-4). 151-159.

Loureiro, A. C. et al. (2020). Assessment of the potential of residues (peel, skin and ends) from the processing of cassava (Manihot esculenta Crantz) for the production of bioethanol using acid hydrolysis. Brazilian Applied Science Review. 4(2).

Nelson, N. (1944). A photometric adaptation of Somogyi method for the determination of glucose. J. Biol. Chem, 153(1), 375-380.

Ofori-Boateng, C. & Lee, K. T. (2014). An oil palm-based biorefinery concept for cellulosic ethanol and phytochemicals production: Sustainability evaluation using exergetic life cycle assessment. Applied Thermal Engineering. 62(1), 90-104.

Otekunrin, O. A. & Sawicka, B. (2019). Cassava, A 21 st Century Staple Crop: How can Nigeria Harness its Enormous Trade Potentials? Acta Scientific Agriculture. 3(8), 194–202.

Palmqvist, E. & Hahn-Hägerdal, B. (2000). Fermentation of lignocellulosic hydrolysates. II: inhibitors and mechanisms of inhibition. Bioresource Technology. 74(1), 25-33.

Pampulha, M. & Loureiro-Dias, M. (1989). Combined effect of acetic acid, pH and ethanol on intracellular pH of fermenting yeast. Appl. Microbiol. Biotechnol. 31(5), 547-550.

Park, J. K. & Chang, H. N. (2000). Microencapsulation of microbial cells.biotechnology Advances. 18, 303-313.

Polachini, T. C. et al. (2020). Chemical composition of lignocellulosic biomass from cassava bagasse and cassava peel: influence of particle size distribution. Advances in Research and Innovations in Chemical Engineering. Atena Editora, 1, 196-208.

Silva, M. D. et al. (2020). Acid Hydrolysis of corn cob for the production of second generation ethanol by Saccharomyces cerevisiae ATCC 26602. International Journal of Development Research. 10(8), 38871-38878.

Somogyi, M. (1945). A new reagent for determination of sugars. J. Biol. Chem. 160(1), 61-68.

Tampion, J. & Tampion, M. D. (1988). Immobilized cells: principles and applications. Cambridge University Press. 257p.,1988.

Tomás-Pejó, E. et al. (2011). Pretreatment Technologies for Lignocellulose-to-Bioethanol Conversion. Biofuels: Alternative Feedstocks and Conversion Processes. Madrid: Elsevier, 149- 176.

Toogood, H. S. & Scrutton, N. S. (2018). Discovery, characterization, engineering, and applications of ene-reductases for industrial biocatalysis. ACS Catal.




How to Cite

SILVA, M. D. da; CANO, J. P.; GARCIA-CRUZ, C. H. Cassava hydrolysates used as substrates for the production of second-generation ethanol by Saccharomyces cerevisiae ATCC 26602 immobilized in sodium alginate spheres . Research, Society and Development, [S. l.], v. 13, n. 3, p. e9613345355, 2024. DOI: 10.33448/rsd-v13i3.45355. Disponível em: Acesso em: 15 jun. 2024.



Agrarian and Biological Sciences