Standardization of an analytical method to quantify ochratoxin A in green coffee beans by high performance liquid chromatography
DOI:
https://doi.org/10.33448/rsd-v9i8.5070Keywords:
HPLC; Fungus; Toxin; Food safety.Abstract
Nowadays, Brazil is the largest producer and exporter of coffee, also the second largest consumer of the beverage. The importance of ensuring food safety for consumers has influenced research to improve and monitor the final product quality. Coffee is a product that presents a high risk of fungal contamination, which can result in the presence of mycotoxins and poses risks to human and animal health. Therefore, this study aimed to standardize a chromatographic method to test and quantify ochratoxin A in 13 samples of green coffee beans. The green coffee beans were stored in sheds without temperature or humidity control. Samples were ground, and the analyte was extracted by a 3% methanol:sodium bicarbonate (1:2 v/v) solution. Ochratoxin A was quantified in a high performance liquid chromatograph. The method was validated by testing the selectivity, linearity, accuracy, limits of detection and quantification. The method presented robustness to the tested parameters and among the analyzed samples. Ochratoxin A was detected above the limit established by the legislation (75.19 µg kg-1) only in one sample. Overall, the storage of green coffee beans in these sheds was adequate, since 12 samples had a low content of ochratoxin A and they were within the limit established by legislation. Therefore, food safety was guaranteed without any severe mycotoxin contamination.
References
Amezqueta, S., Schorr-Galindo, S., Murillo-Arbizu, M., Gonzalez-Peñas, E., De Cerain, A. L., & Guiraud, J. P. (2012). OTA-producing fungi in foodstuffs: a review. Food Control, 26(2), 259-268.
Barcelo, J. M., Barcelo, R. C., & Alvarez, A. (2017). Ochratoxin A, fungal contamination and antioxidant property of defective Arabica coffee in Benguet, Philippines. Emirates Journal of Food and Agriculture, 10-17.
Batista, L. R., & Chalfoun, S. M. (2007). Incidência de Ocratoxina A em diferentes frações do café (Coffea arabica L.): bóia, mistura e varrição após secagem em terreiros de terra, asfalto e cimento. Ciência e Agrotecnologia, 31(3), 804-813.
Benites, A. J., Fernandes, M., Boleto, A. R., Azevedo, S., Silva, S., & Leitão, A. L. (2017). Occurrence of ochratoxin A in roasted coffee samples commercialized in Portugal. Food control, 73, 1223-1228.
Bennet, J. W., and M. Klich. 2003. Mycotoxins. Clin. Microbiol. Rev, 16, 497-516.
Brasil. (2003). Agência Nacional de Vigilância Sanitária ANVISA (2003). Guia para avaliação de métodos analíticos e bioanalíticos. RE n° 899, 1-12.
Brasil. (2011). Agência Nacional de Vigilância Sanitária (ANVISA). Diário oficial da união. Resolução - RDC No - 7, de 18 de fevereiro de 2011. Dispõe sobre limites máximos tolerados (LMT) para micotoxinas em alimentos.
Brasil. (2017). Agência Nacional de Vigilância Sanitária (ANVISA). Diário oficial da união. Resolução - RDC No - 138 de 8 de fevereiro de 2017. Dispõe sobre limites máximos tolerados (LMT) para micotoxinas em alimentos.
Casal, S., Vieira, T., Cruz, R., & Cunha, S. C. (2014). Ochratoxin A in commercial soluble coffee and coffee substitutes. Food research international, 61, 56-60.
Codex alimentarius (2019) General standard for contaminant sand toxins in food and feed. CXS 193-1995, 1-66.
Das Chagas Cardoso Filho, F., de Caldas, M. L., & Muratori, M. C. S. (2015). Fungos e aflatoxinas em cereais: Uma revisão. Revista de Ciência Veterinária e Saúde Pública, 2(2), 122-130.
European Food Safety Authority (EFSA). (2004). Opinion of the Scientific Panel on contaminants in the food chain [CONTAM] related to ochratoxin A (OTA) as undesirable substance in animal feed. EFSA Journal, 2(10), 101.
Ferreira, D. F. (2011). Sisvar: a computer statistical analysis system. Ciência e agrotecnologia, 35(6), 1039-1042.
Galarce-Bustos, O., Alvarado, M., Vega, M., & Aranda, M. (2014). Occurrence of ochratoxin A in roasted and instant coffees in Chilean market. Food control, 46, 102-107.
Harris, D. C. (2008). Análise Química Quantitativa, trad. Jairo Bordinhão [et al.], LTC: Rio de Janeiro.
Iamanaka, B. T., Oliveira, I. S., & Taniwaki, M. H. (2013). Micotoxinas em alimentos. Anais da Academia Pernambucana de Ciência Agronômica, 7, 138-161.
Jeleń, H. H., & Grabarkiewicz-Szczȩsna, J. (2005). Volatile compounds of Aspergillus strains with different abilities to produce ochratoxin A. Journal of agricultural and food chemistry, 53(5), 1678-1683.
Magan, N., Medina, A., & Aldred, D. (2011). Possible climate‐change effects on mycotoxin contamination of food crops pre‐and postharvest. Plant pathology, 60(1), 150-163.
Medina, B. G. (2015). Avaliação da contaminação por Ocratoxina A em amostras de café torrado comercializadas no Brasil.
Oliveira, G., Evangelista, S. R., Passamani, F. R. F., Santiago, W. D., das Graças Cardoso, M., & Batista, L. R. (2019). Influence of temperature and water activity on Ochratoxin A production by Aspergillus strain in coffee south of Minas Gerais/Brazil. LWT, 102, 1-7.
Passamani, F. R. F., Hernandes, T., Lopes, N. A., Bastos, S. C., Santiago, W. D., CARDOSO, M. D. G., & Batista, L. R. (2014). Effect of temperature, water activity, and pH on growth and production of ochratoxin A by Aspergillus niger and Aspergillus carbonarius from Brazilian grapes. Journal of food protection, 77(11), 1947-1952.
Pereira, V. L., Fernandes, J. O., & Cunha, S. C. (2014). Mycotoxins in cereals and related foodstuffs: A review on occurrence and recent methods of analysis. Trends in Food Science & Technology, 36(2), 96-136.
Prelle, A., Spadaro, D., Denca, A., Garibaldi, A., & Gullino, M. L. (2013). Comparison of clean-up methods for ochratoxin A on wine, beer, roasted coffee and chili commercialized in Italy. Toxins, 5(10), 1827-1844.
Ribani, M., Bottoli, C. B. G., Collins, C. H., Jardim, I. C. S. F., & Melo, L. F. C. (2004). Validação em métodos cromatográficos e eletroforéticos. Química nova, 27(5), 771-780.
Soleimany, F., Jinap, S., & Abas, F. J. F. C. (2012). Determination of mycotoxins in cereals by liquid chromatography tandem mass spectrometry. Food chemistry, 130(4), 1055-1060.
Vanesa, D., & Ana, P. (2013). Occurrence of Ochratoxin A in coffee beans, ground roasted coffee and soluble coffee and method validation. Food Control, 30(2), 675-678.
Vecchio, A., Mineo, V., & Planeta, D. (2012). Ochratoxin A in instant coffee in Italy. Food Control, 28(2), 220-223.
World Health Organization, & International Agency for Research on Cancer. (1993). Some naturally occurring substances: food items and constituents, heterocyclic aromatic amines and mycotoxins. IARC Monographs on the Evaluation of the Carcinogenic Risk of Chemicals to Humans, 56.489-421
Yashin, A., Yashin, Y., Xia, X., & Nemzer, B. (2017). Chromatographic methods for coffee Analysis: A Review. Journal of Food Research, 6(4), 60-82.
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