X-ray energy dispersive spectroscopy (EDS) coupled with scanning electron microscope (SEM): fundamentals and applications in dairy products
DOI:
https://doi.org/10.33448/rsd-v10i10.18622Keywords:
EDS; MEV; Dairy products; Elemental analysis.Abstract
Due to the increase in demand for dairy products, the study of their chemical compositions and the changes that occur during storage is extremely important. Therefore, it is necessary to develop efficient analytical techniques in the investigation of the constituent elements of such products, and X-ray energy dispersive spectroscopy (EDS) coupled to the scanning electron microscope (SEM) is a technique that is becoming explored in this context. Due to the lack of published works involving the aforementioned technique in the characterization of dairy products, this work aimed to expand the knowledge about the method in the dairy area. When analyzing dairy samples, SEM provides valuable information about particle morphology, which is critical in choosing target particles. Subsequently, through EDS, the elemental analysis of the surface of the target particles is performed. In the scientific literature, the use of EDS coupled with SEM to characterize samples of powdered milk is observed, with the predominance of the elements carbon and oxygen being reported, in addition to the presence of minerals. Still, the concentrations of carbon, oxygen and nitrogen, obtained through EDS, could be applied in a matrix formula that allowed the calculation of the surface content of constituent compounds of the samples, that is, proteins, lactose and fat. Therefore, from what was exposed in the course of the work, it can be concluded that EDS coupled to SEM is a microanalysis technique with great potential to be further explored and used in the elementary characterization of the surface of particles that make up dairy samples.
References
Daly, K., Fenton, O., Ashekuzzaman, S. M., & Fenelon, A. (2019). Characterisation of dairy processing sludge using energy dispersive X-ray fluorescence spectroscopy. Process Safety and Environmental Protection, 127, 206–210.
Damodaran, S., Parkin, K. L., & Fennema, O. R. (2010). Química de Alimentos de Fennema. Artmed.
Dedavid, B. A., Gomes, C. I., Machado, G. (2007). Microscopia Eletrônica de Varredura - Aplicações e preparação de amostras: materiais poliméricos, metálicos e semicondutores. EDIPUCRS.
Deng, Y., Man, C., Fan, Y., Wang, Z., Li, L., Ren, H., Cheng, W., & Jiang, Y. (2015). Preparation of elemental selenium-enriched fermented milk by newly isolated Lactobacillus brevis from kefir grains. International Dairy Journal, 44, 31–36.
Duarte, L. C., et al. (2003). Aplicações de Microscopia Eletrônica de Varredura (MEV) e Sistema de Energia Dispersiva (EDS) no Estudo de Gemas: exemplos brasileiros. Pesquisas em Geociências, 30(2), 3-15.
Espindola, W. R., Nascente, E. de P., Urzêda, M., Teodoro, J. V. da S., Gonçalves, G. B., Castro, R. D. de, Martins, M. E. P., & Souza, W. J. de. (2020). Quality of refrigerated raw milk produced in the microregion of Pires do Rio, Goiás, Brazil. Research, Society and Development, 9(7), e153973958. https://doi.org/10.33448/rsd-v9i7.3958
Ferrer-Eres, M.A. et al. (2010). Archaeopolymetallurgical study of materials from an Iberian culture site in Spain by scanning electron microscopy with X-ray microanalysis, chemometrics and image analysis. Microchemical Journal, 95, 298-305.
Gandhi, G., Amamcharla, J. K., & Boyle, D. (2017). Effect of milk protein concentrate (MPC80) quality on susceptibility to fouling during thermal processing. LWT - Food Science and Technology, 81, 170–179.
Goldstein, J., Newbury, D.E., Echlin, P., Joy, D.C., Lyman, C.E., Lifshin, E., Linda Sawyer, L., Michael, J.R., (2003). Scanning Electron Microscopy and X-ray Microanalysis. Plenum Press.
Hussain, I., Bell, A. E., & Grandison, A. S. (2013). Mozzarella-Type Curd Made from Buffalo, Cows’ and Ultrafiltered Cows’ Milk: 2. Physicochemical Properties, Curd Yield and Quality, Casein Fractions and Micelle Size. Food and Bioprocess Technology, 6(7), 1741–1748.
Ismail, A. F., Khulbe, K. C., Matsuura, T., Ismail, A. F., Khulbe, K. C., & Matsuura, T. (2019). RO membrane characterization. Reverse Osmosis; Elsevier: Amsterdam, The Netherlands, 57-90.
Jesus, E. L. de, Berndt, F. M., Meneguelli, M., & Muniz, I. M. (2020). Physical chemical characteristics of cooled raw milk under federal inspection. Research, Society and Development, 9(3), e64932302. https://doi.org/10.33448/rsd-v9i3.2302
Kenneth P. Severin. (2004). Dispersive Spectrometry of Common Rock. https://link-springer-com.pbidi.unam.mx:2443/content/pdf/10.1007%2F978-1-4020-2841-0.pdf
Kim, E. H. J., Chen, X. D., & Pearce, D. (2009). Surface composition of industrial spray-dried milk powders. 2. Effects of spray drying conditions on the surface composition. Journal of Food Engineering, 94(2), 169–181.
Lima, E. de A., Botteon, R. de C. C. M., Baroni, F. de A., & Lima, A. C. P. (2020). Influence of filamentous fungi and yeasts on bacterial count and somatic cells in samples of raw bovine milk. Research, Society and Development, 9(7), e304974135. https://doi.org/10.33448/rsd-v9i7.4135
Materials Evaluation and Engineering, Inc. (2016). Energy Dispersive x-ray Spectroscopy (EDS). Handbook of Analytical Methods for Materials, 15-16.
Murrieta-Pazos, I., Gaiani, C., Galet, L., Calvet, R., Cuq, B, & Scher, J. (2012). Food powders: Surface and form characterization revisited. Journal of Food Engineering, 112, 1–21.
Manske, G. A., Danieli, B., Zuffo, G. R., Rigo, E., Gomes, F. J., Zampar, A., & Schogor, A. L. B. (2020). Occurrence of Unstable non-acid milk (UNAM) on commercial farms in the extreme west of Santa Catarina. Research, Society and Development, 9(7), e715974654. https://doi.org/10.33448/rsd-v9i7.4654
Murrieta-Pazos, I., Gaiani, C., Galet, L. & Scher, J. (2012). Composition gradient from surface to core in dairy powders: Agglomeration effect. Food Hydrocolloids, 26, 149–158.
Murrieta-Pazos, I., Galet, L., Rolland, C., Scher, J., & Gaiani, C. (2013). Interest of energy dispersive X-ray microanalysis to characterize the surface composition of milk powder particles. Colloids and Surfaces B: Biointerfaces, 111, 242–251.
Oliveira, F. C. S. de, Farias, L. C. B., Carmo, R. M. do, Oliveira, L. A. de, Santos, G. de O., Silva, M. S., Leão, P. V. T., Cunha, J. V. T. da, Medeiros, J. S., Nicolau, E. S., & Silva, M. A. P. da. (2020). Physico-chemical and sensory characteristics of fresh cheeses fermented with Milk kefir and water kefir. Research, Society and Development, 9(4), e153943015. https://doi.org/10.33448/rsd-v9i4.3015
Parween, R. et al. (2016). Elemental Analysis of Cow’s Milk Applying SEM-EDX Spectroscopy Technique. FUUAST Journal of Biology, 6(2), 161-164.
Pattaro, L., Silva, J. A. G. e, Farias, L. C. B., Medeiros, J. S., Teixeira, P. C., Cunha, J. V. T. da, More, J. C. R. S., Almeida, T. V. de, Nicolau, E. S., & Silva, M. A. P. da. (2020). Physico-chemical and sensory analyzes of milk smoothies of diferente species fermented by kefir, flavored with banana and apple. Research, Society and Development, 9(5), e112953145. https://doi.org/10.33448/rsd-v9i5.3145
Pereira, A. S., Shitsuka, D. M., Parreira, F. J., & Shitsuka, R. (2018). Metodologia da pesquisa científica. 1 edição. UAB/NTE/UFSM.
Siegbahn, K. et al. (1967). ESCA: Atomic, Molecular and Solid State Structure Studied by means of Electron Spectroscopy, Uppsala: Almquist & Wiksells.
Scoutaris, N. et al. (2014). SEM/EDX and confocal Raman microscopy as complementary tools for the characterization of pharmaceutical tablets. International Journal of Pharmaceutics, 470, 88-98.
Toledo, P. H. D. M., Pereira, J. P. F., Perrone, Í. T., Carvalho, A. F., Oliveira, L. F. C., & Stephani, R. (2020). Caracterização da superfície de partículas de produtos lácteos desidratados. Revista do Instituto de Laticínios Cândido Tostes, 75(1), 10–21.
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Copyright (c) 2021 Maria Eduarda Martins Vieira; Mariana Leite Simões e Silva; Luiz Fernando Cappa de Oliveira; Ítalo Tuler Perrone; Rodrigo Stephani
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