Microbiological evaluation of cellular devices as a source of propagations of bacterial resistance
DOI:
https://doi.org/10.33448/rsd-v11i3.26759Keywords:
Cellular device; Biofilm; Multi-resistance; Virulence.Abstract
Cell phones are an important social tool in everyday life, as they facilitate communication processes and allow people to approach quickly and efficiently. Its indiscriminate use in clinical environments by health professionals can contribute to the phenomenon of bacterial resistance, since it serves as a carrier of several microorganisms, and among them they can contain some with high pathogenicity and that present intrinsic virulence mechanisms, such as formation of Biofilms. Therefore, the objective of the present study was to analyze the profile of bacterial isolates from cell phones of health professionals from different sectors in a hospital of Recife, Pernambuco-Brazil, taking into account their resistance profile and their virulence factor, such as biofilm formation. Microbiological data from 10 isolates from cellular devices of health professionals were investigated. The isolates were seeded in specific culture media and the potential for biofilm formation was evaluated on Congo red agar and the isolates were characterized for their antimicrobial resistance profile. As a result, a profile of contamination of cell phones by resistant microorganisms and biofilm producers was obtained, as well as alarming data on the spread of bacterial resistance. In view of the results found, it is concluded that cell phones are important sources of propagation of bacterial resistance, requiring better hygiene processes for their use in the clinical and therapeutic environment, so that they do not put patients in hospital beds at risk. ICU's.
References
Andrade, A. N. A., et al. (2017). Avaliação de parâmetros físico-químicos e eficácia antimicrobiana de saneantes domissanitários de abrangência local e nacional. http://periodicos.ces.ufcg.edu.br/periodicos/index.php/99cienciaeducacaosaude25/article/view/153.
ANVISA, Agência Nacional de Vigilância Sanitária. (2019)."Boletim Segurança do Paciente e Qualidade em Serviços de Saúde no 16. Avaliação dos indicadores nacionais das Infecções Relacionadas à Assistência à Saúde (IRAS) e Resistência microbiana do ano de 2016. https://www.gov.br/anvisa/pt-br/centraisdeconteudo/publicacoes/servicosdesaude/publicacoes
Araújo, L. A. V., et al. (2020). Occurrence and Diversity of Intra-and Interhospital Drug-Resistant and Biofilm-Forming Acinetobacter baumannii and Pseudomonas aeruginosa. Microbial Drug Resistance, 26(7), 802-814. 10.1089/mdr.2019.0214.
Bengoechea, J. Á., & Sá Pessoa, J. (2019). Klebsiella pneumoniae infection biology: living to counteract host defences. FEMS microbiology reviews, 43 (2), 123-144. 10.1093/femsre/fuy043.
Branquinho, P. H. P., & Ferreira, S. C. (2019). Análise microbiologia de superfície em aparelhos celulares da comunidade universitária da unincor com ênfase em Staphylococcus aureus. Revista de Iniciação Científica da Universidade Vale do Rio Verde, 8 (2).
Carneiro, L. L. R. (2013). Smartphones e Tablets para Profissionais de Saúde. TI Medicina, 53. https://www.teses.usp.br/teses/disponiveis/22/22132/tde-07012015-154330/publico/VALTUIRDUARTEDESOUZAJUNIOR.pdf
Chessa, D., et al. (2016). Staphylococcus aureus and Staphylococcus epidermidis virulence strains as causative agents of persistent infections in breast implants. PLoS One, 11 (1). 10.1371/journal.pone.0146668. eCollection 2016.
CLSI. (2018). Performance standards for antimicrobial susceptibility testing. (28th ed.). CLSI supplement M100. Wayne, PA: Clinical and Laboratory Standards institute.
Costa, M., & Silva, W. N. (2018). Investigação dos principais micro-organismos responsáveis por infecções nosocomiais em UTIs neonatais: uma revisão integrativa. Revista Eletrônica da Faculdade de Ceres, 7(1),1-27. https://doi.org/10.36607/refacer.v7i1.3319
European Centre for Disease Prevention and Control (ECDC) (2016). Point prevalence survey of healthcare associated infections and antimicrobial use in European acute care hospitals. https://ecdc.europa.eu/sites/portal/files/media/en/publications/Publications/healthcare-associated-infections-antimicrobial-use-PPS.pdf
Freeman, D. J., Falkiner, F. R., & Keane, C. T. (1989). New method for detecting slime production by coagulase negative staphylococci. Journal Clinical Pathology, 42 (8), 872-4. 10.1136/jcp.42.8.872
Freitas, V. R., & Picoli, S. U. (2007). A coloração de Gram e as variações na sua execução. Revista Newslab, 82, 124-128.
Hage, J. E., Schoch, P., & Cunha, B. A. (2013). Pseudomonas pseudoalcaligenes peritoneal dialysis-associated peritonitis. Peritoneal Dialysis International, 33 (2), 223-224. 10.3747/pdi.2012.00112
Jõesaar, M., et al. (2017). Strategy of Pseudomonas pseudoalcaligenes C70 for effective degradation of phenol and salicylate. PloS one, 12 (3), 173-180. https://doi.org/10.1371/journal.pone.0173180
Laila, H. J. E. A., & Santos, R.C.V. (2016). General aspects and molecular mechanisms involved in the formation of biofilms by Pseudomonas aeruginosa. Disciplinarum Scientia, (17), 125-144.
Loucif, L., et al. (2017). First detection of VIM-2 metallo-β-lactamase-producing Pseudomonas putida in Blattella germanica cockroaches in an Algerian hospital. Antimicrobial agents and chemotherapy, 61 (8), 1-3. 10.1128/AAC.00357-17
Loureiro, R. J., et al. (2016). O uso de antibióticos e as resistências bacterianas: breves notas sobre a sua evolução. Revista Portuguesa de saúde pública, 34 (1) ,77-84.
Macedo, A. J. R. (2019). Prevalência de infecções microbianas nas unidades de terapia intensiva neonatal de dois hospitais de referência da região norte do Ceará. https://repositorio.ufc.br/handle/riufc/41335.
Magiorakos, A. P. et al. (2012). Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clinical microbiology and infection, 18 (3), 268-281. 10.1111/j.1469-0691201103570.x
Modesto, E. M., & Brito, D.V.D. (2019). Infecções relacionadas à assistência à saúde em recém-nascidos de alto risco: perfil de resistência dos bacilos Gram negativos. Revista Eletrônica Acervo Saúde, 11 (7), 517-517. https://doi.org/10.25248/reas.e517.2019.
Nunes, K.O., & Siliano, P. R. (2016). Identificação de bactérias presentes em aparelhos celulares. Identification of bacteria present of mobile phones. Science in health, 7(1), 22-25.
Oliveira, W. F., et al. (2018). Staphylococcus aureus and Staphylococcus epidermidis infections on implants. Journal of Hospital Infection, 98 (2), 111-117. 10.1016/j.jhin.2017.11.008.
Pinto, G. P. N. M. (2016). Biofilmes e feridas crónicas. Tese de Doutorado. https://bdigital.ufp.pt/bitstream/10284/5816/1/PPG_25983.pdf.
Rocha, C. H. L., Muniz, G. S., & Rocha, F. M. G. (2018). Identificação de bactérias presentes em biofilmes de superfícies metálicas. Revista de Investigação Biomédica, 10 (2), 172-180.
Santos A. K. S., et al. (2016). Microbiological profile of nosocomial infections at intensive care units. Journal of Nursing UFPE/ Revista de Enfermagem UFPE online, 10 (3), 1432-1440. 10.5205/1981-8963.
Silva, F. H.., et al. (2020). Antimicrobial resistance and formation of biofilm in clinics isolates causers of infections related to health care. American Journal of Biotechnology and Bioinformatics, 3 (8), 1-8.
Sousa, A. F. F. L., Oliveira, L. B., & Moura, M. E. B. (2016). Perfil epidemiológico das infecções hospitalares causadas por procedimentos invasivos em unidade de terapia intensiva. Revista Prevenção de Infecção e Saúde, 2 (2), 11-17.
Sousa, M. A. S., et al. (2017). Infecções hospitalares relacionadas a procedimentos invasivos em unidades de terapia intensiva: revisão integrativa. Revista Prevenção de Infecção e Saúde, 3 (3), 49-58.
Teixeira, F. N., & Silva, C. V. (2017). Análise microbiológica em telefones celulares. Revista F@ pciência. Apucarana, 11 (3), 15-24. http://www.fap.com.br/fap-ciencia/11_edicao/003.pdf.
Trentin, D. S., Giordani, R. B., & Macedo, A. J. (2013). Biofilmes bacterianos patogênicos: aspectos gerais, importância clínica e estratégias de combate. Revista Liberato, 14 (22), 214-238. 10.31514/rliberato2013v14n22.p213
Wintersdorff, C. J. H., et al. (2016). Dissemination of antimicrobial resistance in microbial ecosystems through horizontal gene transfer. Frontiers in microbiology, (7), 173-173.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Elaine de Lima e Silva; Luciana da Silva Macedo; Meire dos Santos Falcão de Lima ; Gleiciere Maia Silva
![Creative Commons License](http://i.creativecommons.org/l/by/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.