Actividad antioxidante, inhibición de la enzima convertidora de angiotensina I (ECA) y actividad antibacteriana de hidrolizados de proteína de caseinato de búfalo y sus fracciones
DOI:
https://doi.org/10.33448/rsd-v9i12.10772Palabras clave:
Ultrafiltración; Leche de búfalo; Alimentos funcionales.Resumen
En el presente estudio, los caseinatos de leche de búfala producidos por bromelina, neutrasa, papaína y tripsina fueron ultrafiltrados y se evaluó la actividad antioxidante y antimicrobiana de diferentes fracciones. El potencial biológico se evaluó mediante una serie de mediciones: capacidad de eliminar los radicales del 2,2'-azino-bis (ácido 3-etilbenctiazolín-6-sulfónico), 2,2'-difenil-1-picrilhidrazilo (DPPH) e hidroxilos; quelación del cobre y el hierro; propiedades antidiabéticas; inhibición de la enzima convertidora de angiotensina; y actividad antibacteriana contra las cepas de Escherichia coli ATCC 25922, Listeria monocytogenes ATCC 19114, Salmonella typhimurium ATCC 14028 y Staphylococcus aureus ATCC 25923. Las pruebas de eliminación de radicales hidroxilo y DPPH revelaron un mayor potencial en las fracciones de 3-10 kDa. Se observó una actividad de quelación del hierro >70% en todas las fracciones, incluyendo <3 kDa. La quelación del cobre fue >60% en las fracciones >10 kDa. α La inhibición de la amilasa y la actividad antihipertensiva fue óptima en la fracción <3 kDa. La actividad antibacteriana osciló entre 3,28 y 100% de inhibición contra los microorganismos probados, la fracción <3 kDa mostró un mayor potencial inhibitorio. La actividad antihipertensiva de las fracciones osciló entre el 39,35 y el 89,58%. Todos los tratamientos fueron capaces de producir hidrolizados y fracciones con potencial biológico, por lo que el método de ultrafiltración demostró ser eficaz en la separación de péptidos con diferentes masas molares y su posible uso en la industria alimentaria o farmacéutica.
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Derechos de autor 2020 Wellington Leal dos Santos; Thailan Arlindo da Silva; Patrícia Lins Azevedo do Nascimento; Rosângela Estevão Alves Falcão; João Tiago Correia Oliveira; Keila Aparecida Moreira
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