Atividade antioxidante, inibição da enzima conversora de angiotensina I (ECA) e atividade antibacteriana de hidrolisados proteicos de caseinato de búfalo e suas frações
DOI:
https://doi.org/10.33448/rsd-v9i12.10772Palavras-chave:
Ultrafiltração; Leite de búfalo; Alimentos funcionais.Resumo
No presente estudo, os hidrolisados do caseinato de leite de búfala produzidos por bromelina, neutrase, papaína e tripsina foram ultrafiltrados, e diferentes frações foram avaliadas quanto à atividade antioxidante, inibição da enzima conversora de angiotensina I e antimicrobiana. O potencial biológico foi avaliado através de uma série de atividades: capacidade de remover radicais de 2,2'-azino-bis (ácido 3-etilbenztiazolina-6-sulfônico), 2,2'-difenil-1-picrylhydrazyl (DPPH) e hidroxilas; quelação de cobre e ferro; propriedades antidiabéticas; inibição da enzima conversora de angiotensina; e atividade antibacteriana contra Escherichia coli ATCC 25922, Listeria monocytogenes ATCC 19114, Salmonella typhimurium ATCC 14028 e cepas de Staphylococcus aureus ATCC 25923. Os testes para sequestro dos radicais hidroxila e DPPH revelaram um maior potencial nas frações de 3-10 kDa. A atividade de quelação do ferro >70% foi observada em todas as frações, incluindo <3 kDa. A quelação do cobre foi >60% nas frações >10 kDa. A inibição da α-amilase e atividade anti-hipertensiva foi ótima na fração <3 kDa. A atividade antibacteriana variou entre 3,28 e 100% de inibição contra os microrganismos testados. A fração <3 kDa mostrou um potencial inibitório maior. A atividade anti-hipertensiva das frações variou entre 39,35 e 89,58%. Todos os tratamentos foram capazes de produzir hidrolisados e frações com potencial biológico e, portanto, o método de ultrafiltração prova ser eficaz na separação de peptídeos com diferentes massas molares e uso potencial na indústria alimentícia ou farmacêutica.
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Copyright (c) 2020 Wellington Leal dos Santos; Thailan Arlindo da Silva; Patrícia Lins Azevedo do Nascimento; Rosângela Estevão Alves Falcão; João Tiago Correia Oliveira; Keila Aparecida Moreira
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