Perfil químico, potencial bactericida in vitro y toxicidad contra Artemia salina Leach de aceites esenciales obtenidos de condimentos naturales

Autores/as

DOI:

https://doi.org/10.33448/rsd-v10i2.12898

Palabras clave:

Aceites esenciales; Antimicrobiano; Condimentos.

Resumen

El interés por los aceites esenciales y sus aplicaciones alimentarias ha crecido debido a la reacción negativa de los consumidores a los aditivos químicos sintéticos añadidos intencionalmente en los productos industrializados con el fin de aumentar su vida útil. En este contexto, el presente estudio tenía como objetivo evaluar el perfil químico, la toxicidad para la Artemia salina Leach y la actividad antibacteriana in vitro de los aceites esenciales obtenidos a partir de condimentos naturales en bacterias de importancia clínica y alimentaria. Material vegetal: se obtuvo en el municipio de San Luis-MA. Los aceites esenciales fueron extraídos por hidrodestilación a 100 °C/3 h. Se realizó la metodología Folin Ciocalteau para la determinación de fenólicos totales. El ensayo de toxicidad se realizó utilizando el bioensayo de letalidad Artemia salina Leach. La actividad antimicrobiana siguió la metodologia descrito por el Instituto de Normas Clínicas y de Laboratorio utilizando el Método de Difusión y Dilución de Discos de Caldo para la acción de los aceites esenciales contra la bacteria Escherichia coli, Salmonella sp., Staphylococcus aureus y Bacillus cereus. Se identificó la presencia de clases bioactivas en los materiales vegetales utilizados en esta investigación y los aceites esenciales obtenidos fueron clasificados como no tóxicos en el ensayo de toxicidad, presentando baja letalidad al micro crustáceo Artemia salina Leach. En el ensayo de actividad bactericida, los aceites esenciales de O. vulgare, T. vulgaris, C. zeylanicum mostraron resultados significativos, clasificados como eficientes contra los microorganismos probados. Por último, destacamos el uso de aceites esenciales clasificados como activos y no tóxicos en este estudio como alternativas en el control y combate de microorganismos patógenos que presentan una propuesta de producto natural con bajo coste de obtención y alto potencial de mercado.

Biografía del autor/a

Paulo Victor Serra Rosa, Laboratório de Pesquisa e Aplicação de Óleos Essenciais

Bacharel em biomedicina pela Faculdade Uninassau

Ari Pereira de Araújo Neto , Universidade Federal do Delta do Parnaíba

Doutorando em Biotecnologia

Ana Maria Almeida Silva Carvalho, Universidade Federal do Maranhão

Mestranda em Saúde do Adulto

Leila da Silva Silveira, Universidade Federal do Maranhão

Especialista em hematologia pela Universidade Federal do Goiás.

Ione Rocha Neves, Universidade Federal do Maranhão

Especialização em Educação Profissional na Área de Saúde: Enfermagem.
Especialização em Saúde da Família - Clínica na Atenção Primária.
Especialização em Enfermagem Clínico-cirúrgica na modalidade Residência.

Carlos Eduardo Pereira Conceição , Hospital Guarás

Especialista em Medicina do Trabalho

Francilidia Oliveira Vitorino de Assunção Conceição, Universidade Federal do Maranhão

Mestrando em Odontologia na área de Saúde Coletiva

Ricardo Teixeira de Sousa, LabMed e BioSaude

Especialista em Citologia Clínica

Citas

Achkar, M. T., Novaes, G. M., Silva, M. J. D., & Vilegas, W. (2013). Antioxidant property of phenolic compounds: importance in the diet and food preservation. Journal of the Vale do Rio Verde University, 11 (2), 398-406.

Alfaia, D. P. S. (2016). Phytochemical evaluation, cytotoxic and antimicrobial analysis of the crude ethanolic extract of the leaves of Annona muricata L. (Annonaceae). Amazon Biota (Biote Amazonie, Biota Amazonia, Amazonian Biota), 6 (1), 26-30.

Aligiannis, N., Kalpoutzakis, E., Mitaku, S., & Chinou, I. B. (2001). Composition and antimicrobial activity of the essential oils of two Origanum species. Journal of agricultural and food chemistry, 49 (9), 4168-4170.

Almeida, L. A. D. (2017). Phytochemical characterization of Piper nigrum L.

Andrade, M. A., Cardoso, M. D. G., Batista, L. R., Mallet, A. C. T., & Machado, S. M. F. (2012). Essential oils of Cymbopogon nardus, Cinnamomum zeylanicum and Zingiber officinale: composition, antioxidant and antibacterial activities. Revista Ciência Agronômica, 43 (2), 399-408.

Araujo, P. K. A. quality control of samples of pimpinella anisum l. Commercialized in the city of Palmas.

Atti-Santos, A. C., Rossato, M., Pauletti, G. F., Rota, L. D., Rech, J. C., Pansera, M. R., & Moyna, P. (2005). Physicochemical evaluation of essential oils of Rosmarinus officinalis L. Brazilian Archives of Biology and Technology, 48 (6), 1035-1039.

Bakkali, F., Averbeck, S., Averbeck, D., & Idaomar, M. (2008). Biological effects of essential oils – a review. Food and chemical toxicology, 46 (2), 446-475.

Biazotto, F. D. O. (2014). Antioxidant, anticholinesterase activity and metabolic profile of different types of peppers: Implications for Alzheimer's disease (Doctoral dissertation, University of São Paulo).

Binatti, T. T., Geromel, M. R., & Fazio, M. L. S. (2016). Antimicrobial action of spices on bacterial development. Food Hygiene, 30 (260/261).

Botelho, R. G., Inafuku, M. M., Maranho, L. A., Neto, L. M. Olinda, R. A., Dias, C. T., & Tornisielo, V. L. (2010). Acute and chronic toxicity of neem extract (Azadirachta indica) for Ceriodaphnia dubia. Pesticides: ecotoxicology and environment magazine, 20.

Brazil. Ministry of Health. RDC ANVISA No. 276, of September 22, 2005. Technical regulations for spices, seasonings and sauces. Official Gazette [of the Federative Republic of Brazil], Brasília, DF, 2005. Section 1.

Cardoso, J. F. R., Evangelista, D. W., Viana, E. B., Lima, M. E. F., Soares, B. A., Barreto Junior, C. B., & Danelli, M. G. M. (2005). Evaluation of the toxic effect of Piperine isolated from black pepper (Piper nigrum L) in mice. Rural University Magazine, 25 (1), 85-91.

Carvalho, C. A. D. (2012). Botanical, phytochemical characterization and evaluation of the biological activity of extracts of Piptadenia gonoacantha (Mart.) JF Macbr (FABACEAE).

Castilho, P. C., Savluchinske-Feio, S., Weinhold, T. S., & Gouveia, S. C. (2012). Evaluation of the antimicrobial and antioxidant activities of essential oils, extracts and their main components from oregano from Madeira Island, Portugal. Food Control, 23 (2), 552-558.

Chaves, R. D. S. B. (2019). Evaluation of the larvicidal activity of the crude ethanolic extract and essential oils of the leaves of origanum majorana L. and origanum vulgare L. against Aedes aegypti (Linnaeus, 1762).

Costa, L. M., Resende, O., Gonçalves, D. N., & Sousa, K. A. (2012). Quality of crambe fruits during storage. Revista Brasileira de Sementes, 34 (2), 293-301.

Costa Júnior, P. S. P., Ferreira, M. A., Bishop, A. S. D. R., Vieira, B. B., & Evangelista-Barreto, N. S. (2019). Antimicrobial activity of essential oils of different spices. Hig. Food, 2697-2701.

Costa, I. C. D. (2020). Polymeric nanoparticles filled with Piper nigrum essential oil: chemical and morphological characterization.

Cutrim, E. S. M., Teles, A. M., Mouchrek, A. N., Mouchrek Filho, V. E., & Everton, G. O. (2019). Evaluation of the antimicrobial and antioxidant activity of essential oils and hydroalcoholic extracts of Zingiber officinale (Ginger) and Rosmarinus officinalis (Rosemary). Revista Virtual de Química, 11 (1), 60-81.

Everton, G. O., Rosa, P. V. S., Neves, S. C., Pereira, A. P. M., Lima, E. C. S., Mendonça, I. P., ... & Mouchrek Filho, V. E. (2020). Chemical characterization, antimicrobial activity and toxicity of the essential oils of Pimenta dioica L. and Citrus sinensis L. Osbeck. Research, Society and Development, 9 (7), e803974842-e803974842.

Gessinger, M. M. (2013). Mathematical modeling of the supercritical extraction process of pimpinella anisum l. And study of its antimicrobial activity. Graduation Magazine, 6 (2).

Gomes, E. M. C., Pena, R. D. C. M., & Silva, S. S. S. M. (2016). Phytochemical composition and fungicidal action of crude extracts of Cinnamomum zeylanicum on Quambalaria eucalypti. Amazon Biota (Biote Amazonie, Biota Amazonia, Amazonian Biota), 6 (4), 54-58.

Gomes, E. M. C., Firmino, A. V., Pena, R. D. C. M., & Almeida, S. S. M. D. S. D. (2018). In vitro inhibitory effect of Cinnamomum zeylanicum Blume extracts on the control of Cylindrocladium candelabrum. Forest Science, 28 (4), 1559-1567.

Gonçalves, J. H. T., Santos, A. S., & Morais, H. A. (2015). Antioxidant activity, total phenolic compounds and phytochemical screening of dehydrated condiment herbs. Magazine of the Vale do Rio Verde University, 13 (1), 486-497.

Gonçalves, B. T., Lages, L. Z., Alves, Pic, & Gandra, And the Antibacterial Activity Of Thymus Essential Oil (Thymus Vulgaris L.) In Front Of Food Pathogens.

Hassan, O. M., & Elhassan, I. A. (2017). Characterization of essential oils from fruits of Umbelliferous crop cultivated in Sudan I. Pimpinella anisum L (Anise) and Anethum graveolens L. (Dill). Journal of Pharmacognosy and Phytochemistry, 6 (1), 109-112.

Hyldgaard, M., Mygind, T., & Meyer, R. L. (2012). Essential oils in food preservation: mode of action, synergies and interactions with components of the food matrix. Frontiers in microbiology, 3, 12.

Imtara, H., Al-Waili, N., Aboulghazi, A., Abdellaoui, A., Al-Waili, T., & Lyoussi, B. (2021). Chemical composition and antioxidant content of honey Thymus vulgaris and essential oil of Origanum vulgare; its effect on carbon tetrachloride-induced toxicity. Veterinary World, 14 (1), 292.

Indu, M. N., Hatha, A. A. M., Abirosh, C., Harsha, U., & Vivekanandan, G. (2006). Antimicrobial activity of southern Indian condiments on Escherichia coli, Salmonella, Listeria monocytogenes and Aeromonas hydrophila. Brazilian Journal of Microbiology, 37 (2), 153-158.

Jiménez, M., Domínguez, J. A., Pascual-Pineda, L. A., Azuara, E., & Beristain, C. I. (2018). Elaboration and characterization of O / W cinnamon (Cinnamomum zeylanicum) and black pepper (Piper nigrum) emulsions. Food Hydrocolloids, 77, 902-910.

Liston, M. D. S. (2013). Study of the composition and application of the essential oil of origanum vulgare l. As an antimicrobial agent in sururu (mytella falcata).

Machado, B. A. S., Ribeiro, D. S., & Druzian, J. I. (2013). Prospective study on the antimicrobial activity of some aromatic plants. Prospecting Notebooks, 6 (1), 97.

Menezes Filho, A. C. P., & Souza Castro, C. F. (2019). Phytochemical classes of secondary metabolites in foliar ethanolic extracts of species of the brazilian cerrado. Revista Saúde & Ciência Online, 8 (1), 45-61.

Melo, C. F. M., Furlan Junior, J., & Huhn, S. (1997). Black pepper: essential oil and oleoresin. In Embrapa Amazônia Oriental-Article in congress proceedings (ALICE). In: international seminar on pepper-do-reino and cupuaçu, 1996, Belém, PA. Anais ... Belém, PA: EMBRAPA-CPATU: JICA, 1997.

Mendes, LSDS (2012). Chemical Study and Larvicide Activity in Front Of Aedes Aegypti Of The Essential Oil Of The Leaves Of Cinnamomum Zeylanicum Breyn (Cinnamon).

Moreira, M. R., Ponce, A. G., Del Valle, C. E., & Roura, S. I. (2005). Inhibitory parameters of essential oils to reduce a foodborne pathogen. LWT-Food Science and Technology, 38 (5), 565-570.

National Health Surveillance Agency (2019). Brazilian Pharmacopoeia. (6a ed.), Editora Fio Cruz, 1. 1-546 p.

Porto, L. L., & Rosa, L. R. V. D. (2018). Evaluation of the antimicrobial potential of essential oils of coriander (coriandrum sativum l.) And oregano (origanum vulgare l.) (Bachelor's thesis, Universidade Tecnológica Federal do Paraná).

Pereira, E. M., Leite Filho, M. T., by Assis Mendes, F., Martins, Ana, & Rocha, A. P. T. (2015). Toxicological potential against Artemia Salina in condiment plants sold in the city of Campina Grande, PB. Green Journal of Agroecology and Sustainable Development, 10 (1), 52-56.

Ramos, A. V. G., Eninger, M. C., Tiuman, T. S., & Adamczuck, R. S. S. Antibacterial and cytotoxic activity of essential oil of thyme (thymus vulgaris l.) And evaluation of synergistic effect with synthetic food preservatives. Food, 38.

Reis, J. B., from Figueiredo, L. A., Castorani, G. M., & Veiga, Smom (2020). Evaluation of the antimicrobial activity of essential oils against food pathogens. Brazilian Journal of Health Review, 3 (1), 342-363.

Reis, J. B. (2012). Analytical study, evaluation of toxicity and molluscicidal activity of the essential oil Cinnamomum zeylanicum Blume (cinnamon) against the snail Biomphalaria glabrata (Say, 1818).

Rocha, W. S., Lopes, R. M., Silva, D. B. D., Vieira, R. F., Silva, J. P. D., & Agostini-Costa, T. D. S. (2011). Total phenolic compounds and condensed tannins in native fruits of the cerrado. Revista Brasileira de Fruticultura, 33 (4), 1215-1221.

Salviano, L. F., Geromel, M. R., & Fazio, M. L. S. (2017). Antibacterial activity of essential oils of green and roasted coffee (Coffea arabica), Cocoa (Theobroma cacao), Ceylon cinnamon bark and leaf (Cinnamomum zeylanicun). Hig. Food, 107-111.

Santin, R. (2013). Antifungal potential and toxicity of essential oils of the Lamiaceae family.

Santos, R. D. (2009). Phenolic compounds of lamiaceae herbs in the oxidative stability of butter and evaluation of the toxicity of rosemary extract (Rosemarinus officinalis l.).

Santos, E. J. D. (2014). Effect of ingestion of cinnamon tea C. burmannii on postprandial glycemia in non-diabetic adult individuals (Doctoral dissertation).

Silva, C. A. L., Amaral, C. A., Santos, C. M., Leite, D. S., & Santos Brasil, J. (2019). Antimicrobial activity of plants of popular use in Brazil: chamomile (matricaria chamomilla), fennel (pimpinella anisum) and jucá (caesalpinia ferrea). Antimicrobial activity of plants of popular use in Brazil: chamomile (matricaria chamomilla), fennel (pimpinella anisum) and jucá (caesalpinia ferrea), 1-388.

Santos, J. R. N., Teles, A. M., Ferreira, C. G., & Mouchrek, A. N. (2020). Evaluation of the bactericidal and antioxidant activity of the essential oil and the hydroalcoholic extract of oregano (Origanum vulgare). Research, Society and Development, 9 (10), e7829108410-e7829108410.

Santos, R. A. M., & Martins, K. M. (2019). Quality control of vegetable drugs Matricaria recutita L., Peumus boldus M. and Pimpinella anisum L., sold in pharmacies in Maringá-PR. Journal of Biology & Pharmacy And Agricultural Management, 15 (4).

Souza, C. A. S., Almeida, L. N., Santos Cruz, E., Silva, C. M. L., Júnior, J. A. C. N., Silva, F. A., & Serafini, M. R. (2017). Physico-chemical quality control and phytochemical characterization of the main medicinal plants sold at the Lagarto-SE fair. Scientia Plena, 13 (9).

Sarto, M. P. M., & Junior, G. Z. (2014). Antimicrobial activity of essential oils. UNINGÁ Review Magazine, 20 (1).

Schumack, K. D. S. P., Almeida Barbosa, A. C., Santos, L., Santos, T. G., & Lima, C. P. (2017). Antimicrobial activity of the essential oils of Thymus vulgaris L., Eucalyptus citriodora Hook and Illicium verum Hook f. Proceedings of EVINCI-UniBrasil, 3 (1), 244-244.

Simões, C. M. O., Schenkel, E. P., Gosmann, G., Mello, J. C. P., Mentz, L. A., & Petrovick, P. R. (2007). Pharmacognosy: from plant to medicine. 1002 pg. Porto Alegre / Florianópolis: Editora da UFSC / Editora da UFRGS.

Silva, T. B., & Rangel, E. T. (2010). Evaluation of the antimicrobial activity of the ethanol extract of thyme (Thymus vulgaris l.) In vitro. Electronic Pharmacy Magazine, 7 (2), 11-11.

Singh H. P., Kaur S., Negi K., Kumari S., Saini V., Batish, D. R. & Kohli R. K. (2012). Assessment of in vitro antioxidant activity of essential oil of Eucalyptus citriodora (lemon-scented Eucalypt; Myrtaceae) and its major constituents. LWT-Food science and Technology, 48 (2), 237-241.

Soares, V. G. (2020). Content of phenolic compounds and physico-chemical analyzes in different fresh and dehydrated condiments of rosemary, mint, basil and oregano.

Stobart, T. (2018). Herbs, spices and condiments: from A to Z. Editora Schwarcz-Companhia das Letras.

Vieira, R. (2010). Photosensitivity Study of Essential Oil of R osmarinuso ff icinalis Accompanied by UV-VIS Spectroscopy. 2010. 45 f. Course Conclusion Paper - Federal Technological University of Paraná (UTFPR), Toledo.

Viuda-Martos, M., Mohamady, M. A., Fernández-López, J., Abd ElRazik, K. A., Omer, E. A., Pérez-Alvarez, J. A., & Sendra, E. (2011). In vitro antioxidant and antibacterial activities of essentials oils obtained from Egyptian aromatic plants. Food Control, 22 (11), 1715-1722.

Zielinski, A. A. F .Evaluation of phenolic compounds and in vitro antioxidant activity of teas: classification, modeling and optimization by chemometric techniques.

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Publicado

28/02/2021

Cómo citar

MARTINS, T. G. T.; ROSA, P. V. S. .; ARAÚJO NETO , A. P. de; CARVALHO, A. M. A. S. .; SILVEIRA, L. da S. .; NEVES, I. R.; CONCEIÇÃO , C. E. P. .; CONCEIÇÃO, F. O. V. de A. .; SOUSA, R. T. de .; FONSECA, D. .; EVERTON, G. O.; MOUCHREK FILHO, V. E. . Perfil químico, potencial bactericida in vitro y toxicidad contra Artemia salina Leach de aceites esenciales obtenidos de condimentos naturales. Research, Society and Development, [S. l.], v. 10, n. 2, p. e58310212898, 2021. DOI: 10.33448/rsd-v10i2.12898. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/12898. Acesso em: 2 jul. 2024.

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Ciencias de la salud