Actividad fitoquímica, físico-química, microbiológica y anticolinesterasa de Ginkgo biloba L. y Bacopa monnieri L. utilizados en fitoterapia

Autores/as

DOI:

https://doi.org/10.33448/rsd-v10i3.13480

Palabras clave:

Control de calidad; Análisis fitoquímico; Actividad anticolinesterasa; Ginkgo biloba L; Bacopa monnieri L.

Resumen

Los medicamentos a base de hierbas a base de Ginkgo biloba L. y Bacopa monnieri L. se utilizan para mejorar la memoria y la función cognitiva. Se evaluó el control de calidad y la actividad anticolinesterasa de los medicamentos herbarios preparados a partir de G. biloba y B. monnieri vendidos en farmacias de manipulación. Se obtuvieron muestras de medicamentos herbales a base de G. biloba y B. monnieri de las farmacias distribuidoras de Teresina-PI, sometidas a análisis de etiquetado y actividad anticolinesterasa. El estudio fitoquímico se realizó mediante prospección preliminar, CCD y CLAE. Se realizaron pruebas físico-químicas y análisis microbiológicos de acuerdo con Pharmacopéia 2012. Se observó que el etiquetado, material extraño, desintegración y parámetros microbiológicos estaban de acuerdo con las normas ANVISA. Las pruebas colorimétricas fueron uniformes en las muestras de B. monnieri y variables de G. biloba. La evaluación del peso medio mostró que las muestras G1 y B3 tienen un contenido capsular superior al descrito en la etiqueta y presentan diferentes valores de pH. Entre los perfiles termogravimétricos, las pérdidas de masa de las muestras G1 y B3 presentaron valores con alto contenido en cenizas, sugiriendo adulteraciones. La prospección fitoquímica mostró flavonoides como metabolitos secundarios comunes en ambas especies, corroborando con análisis de CCD y HPLC, que identificaron los compuestos ácido clorogénico, rutina, miricetina y quercetina. Las muestras G4, B2 y B5 presentan metabolitos capaces de inhibir la enzima acetilcolinesterasa con IC50 de 0.8540 mg / mL, 0.9650 mg / mL y 1.8221 × 10-5 mg / mL, respectivamente. Las muestras G1 y B3 de G. biloba y B. monnieri, no obedecieron algunos parámetros de control de calidad para medicamentos herbarios según los criterios de la Farmacopea Brasileña.

Biografía del autor/a

Tatiana de Oliveira Lopes, Piaui State University

Chemistry Department, PPGQ -GERATEC- Piaui State University, Teresina, PI, CEP-64002-150, Brazil.

Jaqueline Fernanda de Sousa Silva, Piaui State University

Chemistry Department, PPGQ -GERATEC- Piaui State University, Teresina, PI, CEP-64002-150, Brazil.

Rudielson dos Santos Silva, Piaui State University

Chemistry Department, PPGQ -GERATEC- Piaui State University, Teresina, PI, CEP-64002-150, Brazil.

Renata da Silva Carneiro, Piaui State University

Departamento de Química, PPGQ -GERATEC- Universidade Estadual do Piauí, Teresina, PI, CEP-64002-150, Brasil.

Antônio Rafael de Oliveira, Piaui State University

Chemistry Department, PPGQ -GERATEC- Piaui State University, Teresina, PI, CEP-64002-150, Brazil.

Yáscara Lopes de Oliveira, Piaui State University

Chemistry Department, PPGQ -GERATEC- Piaui State University, Teresina, PI, CEP-64002-150, Brazil.

Renato Pinto de Sousa, Piaui Federal Universit

Chemistry Department, LPN, Piaui Federal Universit, CEP - 64049-550 Teresina, PI, Brazil

Clara Andrezza Crisóstomo Bezerra Costa, Alagoas Federal Institute

Chemistry Department - Alagoas Federal Institute, Maceió, AL, CEP-57035-350, Brazil

 

Iron Jonhson de Araújo Veras, Piaui State University

Biology Department, LABMICRO, Piaui State University, Teresina, PI, CEP-64002-150, Brazil

Ronaldo dos Santos Sousa Júnior, Piaui Federal Universit

Pharmacy Department, LPNNEX, Piaui Federal Universit, CEP - 64049-550 Teresina, PI, Brazil.

Johnnatan Duarte de Freitas , Alagoas Federal Institute

Chemistry Department - Alagoas Federal Institute, Maceió, AL, CEP-57035-350, Brazil

Mahendra Rai, SGB Amravati University

Department of Biotechnology, SGB Amravati University, Amravati, 444 602, Maharashtra State, India

Laécio Santos Cavalcante, Piaui State University

Chemistry Department, PPGQ -GERATEC- Piaui State University, Teresina, PI, CEP-64002-150, Brazil.

Francisca Lucia de Lima, Piaui State University

Biology Department, LABMICRO, Piaui State University, Teresina, PI, CEP-64002-150, Brazil.

Mariana Helena Chaves, Piaui Federal Universit

Chemistry Department, LPN, Piaui Federal Universit, CEP - 64049-550 Teresina, PI, Brazil.

Chistiane Mendes Feitosa, Piaui Federal Universit

Pharmacy Department, LPNNEX, Piaui Federal Universit, CEP - 64049-550 Teresina, PI, Brazil.

Valdiléia Teixeira Uchôa, Piaui State University

Chemistry Department, PPGQ -GERATEC- Piaui State University, Teresina, PI, CEP-64002-150, Brazil.

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Publicado

20/03/2021

Cómo citar

LOPES, T. de O. .; SILVA, J. F. de S. .; SILVA, R. dos S. .; CARNEIRO, R. da S. .; OLIVEIRA, A. R. de .; OLIVEIRA, Y. L. de .; SOUSA, R. P. de .; COSTA, C. A. C. B. .; VERAS, I. J. de A. .; SOUSA JÚNIOR, R. dos S. .; FREITAS , J. D. de .; RAI, M.; CAVALCANTE, L. S. .; LIMA, F. L. de .; CHAVES, M. H. .; FEITOSA, C. M. .; UCHÔA, V. T. . Actividad fitoquímica, físico-química, microbiológica y anticolinesterasa de Ginkgo biloba L. y Bacopa monnieri L. utilizados en fitoterapia. Research, Society and Development, [S. l.], v. 10, n. 3, p. e39010313480, 2021. DOI: 10.33448/rsd-v10i3.13480. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/13480. Acesso em: 23 nov. 2024.

Número

Vol. 10 Núm. 3

Sección

Ciencias de la salud

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Derechos de autor 2021 Tatiana de Oliveira Lopes; Jaqueline Fernanda de Sousa Silva; Rudielson dos Santos Silva; Renata da Silva Carneiro; Antônio Rafael de Oliveira; Yáscara Lopes de Oliveira; Renato Pinto de Sousa; Clara Andrezza Crisóstomo Bezerra Costa; Iron Jonhson de Araújo Veras; Ronaldo dos Santos Sousa Júnior; Johnnatan Duarte de Freitas ; Mahendra Rai; Laécio Santos Cavalcante; Francisca Lucia de Lima; Mariana Helena Chaves; Chistiane Mendes Feitosa; Valdiléia Teixeira Uchôa

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