Cytotoxicity and osteogenic potential of experimental medication with calcium hydroxide and activated charcoal
DOI:
https://doi.org/10.33448/rsd-v10i5.14671Keywords:
Alkaline phosphatase; Calcium hydroxide; Charcoal; Endodontics.Abstract
Objectives: The aim of this study was to evaluate the cytotoxicity and osteogenic potential of experimental medications containing Calcium Hydroxide (CH) and Activated charcoal (AC). Methodology: Osteoblastic (MC3T3) and fibroblastic (L929) cells were cultivated in 96 well plates (1 x 104 cells/well) and, after 24 h, treated with extracts, according to experimental groups [(Experimental groups: C - Control; CH - CH paste; CH+10%AC - experimental paste 1 (CH paste + 10% AC); CH+5%AC - experimental paste 2 (CH paste + 5% AC)]. Cytotoxicity and potential osteogenic was performed by MTT and alkaline phosphatase activity, respectively, after 1, 3 and 7 days. Results: For intergroups comparisons, ANOVA 2 factors were used, followed by Tukey test (p < 0.05). There was no difference among the pastes for cytotoxicity in both cells (p > 0.05). For osteogenic potential, it was found that all experimental groups stimulated mineralization in relation to control group, except for experimental paste 2 at 7 days. Conclusion: The addition of AC to the CH paste does not modify toxicity and osteogenic properties. Add 10% of AC appears to be more effective than 5%.
References
Althumairy, R. I., Teixeira, F. B., & Diogenes, A. (2014). Effect of dentin conditioning with intracanal medicaments on survival of stem cells of apical papilla. J. Endod, 40(4):521-5. https://doi.org/10.1016/j.joen.2013.11.008
Ballini, A., Cantore, S., Saini, R., Pettini, F., Fotopoulou, E. A., Saini, S. R., Georgakopoulos, I. P., Dipalma, G., Gargiulo Isacco, C., & Inchingolo, F. (2019). Effect of activated charcoal probiotic toothpaste containing Lactobacillus paracasei and xylitol on dental caries: a randomized and controlled clinical trial. Journal of biological regulators and homeostatic agents, 33(3), 977–981.
Carvalho, G. A. O., de Almeida, R. R., Câmara, J. V. F., & Pierote, J. J. A. (2020). Hidróxido de cálcio versus hibridização em capeamentos pulpares: revisão de literatura. Research, Society and Development, 9(7), e244974069-e244974069. http://dx.doi.org/10.33448/rsd-v9i7.4069
Carvalho, N. C., Guedes, S., Albuquerque-Júnior, R., de Albuquerque, D. S., de Souza Araújo, A. A., Paranhos, L. R., Camargo, S., & Ribeiro, M. (2018). Analysis of Aloe vera cytotoxicity and genotoxicity associated with endodontic medication and laser photobiomodulation. Journal of photochemistry and photobiology. B, Biology, 178, 348–354. https://doi.org/10.1016/j.jphotobiol.2017.11.027
Chan, W., Chowdhury, N. R., Sharma, G., Zilm, P., & Rossi-Fedele, G. (2020). Comparison of the biocidal efficacy of sodium dichloroisocyanurate and calcium hydroxide as intracanal medicaments over a 7-day contact time: an ex vivo study. Journal of Endodontics, 46(9), 1273-1278. https://doi.org/10.1016/j.joen.2020.05.011
Chen, L., Zheng, L., Jiang, J., Gui, J., Zhang, L., Huang, Y., Chen, X., Ji, J. & Fan, Y. (2016). Calcium Hydroxide–induced Proliferation, Migration, Osteogenic Differentiation, and Mineralization via the Mitogen-activated Protein Kinase Pathway in Human Dental Pulp Stem Cells. J. Endod, 42(9):1355-61. https://doi.org/10.1016/j.joen.2016.04.025
Correa, G. T. B., et al (2009). Cytotoxicity evaluation of two root canal sealers and a commercial calcium hydroxide paste on THP1 cell line by Trypan Blue assay. Journal of Applied Oral Science, 17(5), 457-461. https://dx.doi.org/10.1590/S1678-77572009000500020
da Silva, G. F., Cesário, F., Garcia, A., Weckwerth, P. H., Duarte, M., de Oliveira, R. C., & Vivan, R. R. (2020). Effect of association of non-steroidal anti-inflammatory and antibiotic agents with calcium hydroxide pastes on their cytotoxicity and biocompatibility. Clinical oral investigations, 24(2), 757–763. https://doi.org/10.1007/s00784-019-02923-y
de Oliva, M. A., Maximiano, W. M., de Castro, L. M., da Silva, P. E., Jr, Fernandes, R. R., Ciancaglini, P., Beloti, M. M., Nanci, A., Rosa, A. L., & de Oliveira, P. T. (2009). Treatment with a growth factor-protein mixture inhibits formation of mineralized nodules in osteogenic cell cultures grown on titanium. The journal of histochemistry and cytochemistry: official journal of the Histochemistry Society, 57(3), 265–276. https://doi.org/10.1369/jhc.2008.952713
Desai S, Chandler N. (2009). Calcium hydroxide-based root canal sealers: a review. J Endod.35(4):475-80. 10.1016/j.joen.2008.11.026.
Elfaramawy, M. (2021). The Effect Of The Addition Of Activated Charcoal To Different Formulations Of Calcium Hydroxide On Their Effect On The Fracture Resistance Of Endodontically Treated Teeth. Egyptian Dental Journal, 67(2), 1629-1623. 10.21608/edj.2021.48721.1325
Estrela, C., Sydney, G. B., Bammann, L. L., & Felippe Junior, O. (1995). Mechanism of the action of calcium and hydroxy ions of calcium hydroxide on tissue and bacteria. Braz Dent J, 6(2): 85-90. http://143.107.206.201/bdj/t0262.html
Gao, Y., Wang, G., Li, Y., Lv, C., & Wang, Z. (2019). Effects of oral activated charcoal on hyperphosphatemia and vascular calcification in Chinese patients with stage 3-4 chronic kidney disease. Journal of nephrology, 32(2), 265–272. https://doi.org/10.1007/s40620-018-00571-1
Garrocho-Rangel, A., Escobar-García, D. M., Gutiérrez-Sánchez, M., Herrera-Badillo, D., Carranco-Rodríguez, F., Flores-Arriaga, J. C., & Pozos-Guillén, A. (2021). Calcium hydroxide/iodoform nanoparticles as an intracanal filling medication: synthesis, characterization, and in vitro study using a bovine primary tooth model. Odontology, 1-9. https://doi.org/10.1007/s10266-021-00591-7
Giongo, M, Santos, R. A. M. dos, Maciel, S. M, Fracasso, M. L. C, & Victorino, F. R. (2017). Analysis of pH and release of calcium of association between melaleuca alternifolia oil and calcium hydroxide. Revista de Odontologia da UNESP, 46(2), 104-108. Epub March 13, 2017.https://doi.org/10.1590/1807-2577.07816
Hilton, T. J., Ferracane, J. L., Mancl, L., & Northwest Practice-based Research Collaborative in Evidence-based Dentistry (NWP) (2013). Comparison of CaOH with MTA for direct pulp capping: a PBRN randomized clinical trial. Journal of dental research, 92(7), 16S–22S. https://doi.org/10.1177/0022034513484336
Illingworth, J. M., Rand, B., Williams, P. T. (2012). Novel activated carbon fibre matting from biomass fibre waste. Proceedings of the Institution of Civil Engineers-Waste and Resource Management, 165(3):123-132. https://doi.org/10.1680/warm.12.00001
ISO E. Biological evaluation of medical devices-Part 12: Sample Preparation and reference materials. 2012.
ISO, Standardization IOf. ISO 10993-5: Biological evaluation of medical devices-Part 5: Tests for in vitro cytotoxicity. ISO Geneva; 2009.
Kim, H. C., Park, S.J., Lee, C. G., Kim, S. B., Kim, K. W. (2009). Bacterial attachment to iron-impregnated granular activated carbon. Colloids Surf. B: Biointerfaces, 74(1):196-201. https://doi.org/10.1016/j.colsurfb.2009.07.018
Labban, N., Yassen, G. H., Windsor, L. J., & Platt, J. A. (2014). The direct cytotoxic effects of medicaments used in endodontic regeneration on human dental pulp cells. Dental traumatology, 30(6), 429–434. https://doi.org/10.1111/edt.12108
Lim, M.J., Jang, H.J., Yu, M.K., Lee, K.W., Min, K.S. (2017). Removal efficacy and cytotoxicity of a calcium hydroxide paste using N-2-methyl-pyrrolidone as a vehicle. Restor Dent Endod, 42(4):290-300. https://doi.org/10.5395/rde.2017.42.4.290
Modareszadeh, M. R., Di Fiore, P. M., Tipton, D. A., & Salamat, N. (2012). Cytotoxicity and alkaline phosphatase activity evaluation of endosequence root repair material. Journal of endodontics, 38(8), 1101–1105. https://doi.org/10.1016/j.joen.2012.04.014
Mohammadi, Z., & Dummer, P. M. (2011). Properties and applications of calcium hydroxide in endodontics and dental traumatology. International endodontic journal, 44(8), 697–730. https://doi.org/10.1111/j.1365-2591.2011.01886.x
Mosmann T. (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of immunological methods, 65(1-2), 55–63. https://doi.org/10.1016/0022-1759(83)90303-4
Oliveira, I. R., Andrade, T. L., Jacobovitz, M., & Pandolfelli, V. C. (2013). Bioactivity of calcium aluminate endodontic cement. Journal of endodontics, 39(6), 774–778. https://doi.org/10.1016/j.joen.2013.01.013
Pereira, T. C., da Silva Munhoz Vasconcelos, L. R., Graeff, M., Ribeiro, M., Duarte, M., & de Andrade, F. B. (2019). Intratubular decontamination ability and physicochemical properties of calcium hydroxide pastes. Clinical oral investigations, 23(3), 1253–1262. https://doi.org/10.1007/s00784-018-2549-0
Pires, C. W., Botton, G., Cadoná, F. C., Machado, A. K., Azzolin, V. F., da Cruz, I. B., Sagrillo, M. R., & Praetzel, J. R. (2016). Induction of cytotoxicity, oxidative stress and genotoxicity by root filling pastes used in primary teeth. International endodontic journal, 49(8), 737–745. https://doi.org/10.1111/iej.12502
Reddy, S., Prakash, V., Subbiya, A., & Mitthra, S. (2020). 100 Years of Calcium Hydroxide in Dentistry: A Review of Literature. Indian Journal of Forensic Medicine & Toxicology, 14(4), 1203.
Saatchi, M., Shokraneh, A., Navaei, H., Maracy, M. R., & Shojaei, H. (2014). Antibacterial effect of calcium hydroxide combined with chlorhexidine on Enterococcus faecalis: a systematic review and meta-analysis. Journal of Applied Oral Science, 22(5), 356-365. http://dx.doi.org/10.1590/1678-775720140032
Saravanan, A., Kumar, P.S., Devi, G.K., Arumugam, T. (2016). Synthesis and characterization of metallic nanoparticles impregnated onto activated carbon using leaf extract of Mukia maderasapatna: Evaluation of antimicrobial activities. Microb. Pathog, 97:198-203. https://doi.org/10.1016/j.micpath.2016.06.019
Souza-Filho, F. J. de, et al (2008). Antimicrobial effect and pH of chlorhexidine gel and calcium hydroxide alone and associated with other materials. Brazilian Dental Journal, 19(1), 28-33. https://doi.org/10.1590/S0103-64402008000100005
Sugumaran, P., Susan, V. P., Ravichandran, P., & Seshadri, S. (2012). Production and characterization of activated carbon from banana empty fruit bunch and Delonix regia fruit pod. J Sustainable Energy & Environment, 3(3):125-32.
Tanomaru-Filho, M., Guerreiro-Tanomaru, J. M., Faria, G., Aguiar, A. S., & Leonardo, R. T. (2015). Antimicrobial activity and ph of calcium hydroxide and zinc oxide nanoparticles intracanal medication and association with chlorhexidine. The journal of contemporary dental practice, 16(8), 624-629. DOI: 10.5005/jp-journals-10024-1732
Victorino, F. R., Rocha, I. S., de Oliveira Lazarin, R., Seron, M. A., Sivieri-Araujo, G., & Almeida, R. S. (2021). Maxillary Canine with two roots and two canals: A case report. Research, Society and Development, 10(2). DOI: http://dx.doi.org/10.33448/rsd-v10i2.12599
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Copyright (c) 2021 Gabriela Sumie Yaguinum Gonçalves; Danielle Gregorio; Isabelly Ribeiro Custódio; Luciana Prado Maia; Bruno Piazza; Graziela Garrido Mori
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