Citotoxicidade e potencial osteogênico de medicação experimental com hidróxido de cálcio e carvão ativado
DOI:
https://doi.org/10.33448/rsd-v10i5.14671Palavras-chave:
Fosfatase alcalina; Hidróxido de cálcio; Carvão vegetal; Endodontia.Resumo
Objetivos: O objetivo deste estudo foi avaliar a citotoxicidade e o potencial osteogênico de medicamentos experimentais contendo Hidróxido de Cálcio (HC) e Carvão Ativado (CA). Metodologia: Células osteoblásticas (MC3T3) e fibroblásticas (L929) foram cultivadas em placas de 96 poços (1 x 104 células / poço) e, após 24 h, tratadas com extratos, de acordo com os grupos experimentais [(Grupos experimentais: C – Controle; CH - pasta HC; HC + 10% CA - pasta experimental 1 (pasta HC + 10% CA); HC + 5% CA - pasta experimental 2 (pasta HC + 5% CA)]. Citotoxicidade e potencial osteogênico foram realizados por Atividade do MTT e da fosfatase alcalina, respectivamente, após 1, 3 e 7 dias. Resultados: Para as comparações intergrupos, foram utilizados os fatores ANOVA 2, seguido do teste de Tukey (p <0,05). Não houve diferença entre as pastas para citotoxicidade em ambas as células (p> 0,05). Para o potencial osteogênico, verificou-se que todos os grupos experimentais estimularam a mineralização em relação ao grupo controle, exceto a pasta experimental 2 aos 7 dias. Conclusão: A adição de CA na pasta HC não altera a toxicidade e as propriedades. A adição de 10% de CA parece ser mais eficaz do que 5%.
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Copyright (c) 2021 Gabriela Sumie Yaguinum Gonçalves; Danielle Gregorio; Isabelly Ribeiro Custódio; Luciana Prado Maia; Bruno Piazza; Graziela Garrido Mori
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