Propiedades de transdiferenciación, osteoconducción y osteoinducción in vivo de biomateriales orgánicos experimentales soluble en agua – Estudio Piloto
DOI:
https://doi.org/10.33448/rsd-v10i5.15017Palabras clave:
Ingeniería de Tejidos; Orgánica; Biomateriales; Injerto Ectopico; Regeneracíon Ósea; Inmunohistoquímica.Resumen
Matriz ósea inorgánica bovina (IBBM) son biomateriales con características osteocoductoras comprobadas. El objetivo del presente fue evaluar las funcionalidades de regeneración ósea de IBBM modificado por MOE en ovinos. MOE fue sintetizado suspendiendo-se nácar (0.05 g, diámetros < 0.01 mm) en ácido acético anhidro (pH 7, 25° C, 72 horas) con agitación magnética. Tubos huecos de polietileno (d = 5.00 mm, l = 10.00 mm, extremos abiertos) del grupo control negativo (simulacro) o grupos experimentales (IBBM o IBBM modificado por MOE) fueran colocados (n = 3/condición/animal; intramuscular) adyacente a la parte distal de la columna de ovejas (8 animales, » 45 kg, 2 años). Tejidos fueron recogidos (3 o 6 meses) después implantación para análisis histológicos (H), morfométricos (MM) e inmunohistoquímicos (IH; Wnt-3a, CD34, Vimentin y PREF-1). Datos MM se analizaron utilizando las pruebas de Shapiro-Wilk y Levene, Mann Whitney y Kruskal Wallis. Datos IM se analizaron mediante ANOVA de medidas repetidas y Tukey. Diferencias (p < 0.05) ocurrieran entre grupos experimentales (IBBM y IBBM +MOE a los 3 y 6 meses) y controles (simulacro) para área total; no se encontraron diferencias para partículas remanentes entre grupos experimentales; hueso recién formado se produjo solo en la presencia de biomateriales. Maior cantidad de hueso fue observada con IBBM + MOE (6-meses). No hubo diferencias (p > 0.05) en IH (Wnt-3a, CD34, Vimentin y PREF-1). Resultados reportados indican que materiales experimentales (IBBM + MOE) tienen características prometedoras. Se necesitan estudios para definir efectos longitudinales y de biocompatibilidad de los biomateriales.
Citas
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Derechos de autor 2021 Viviane Rozeira Crivellaro; Gilvan Spada; Cláudia Salete Judachesci ; Paula Porto Spada; Luiza Rodrigues Saling; Idélcena Tatiane Miranda; Tatiana Miranda Deliberador; Marilisa Carneiro Leão Gabardo; Rafaela Scariot; Moira Pedroso Leão; Carmen Lucia Mueller Storrer; Sharukh Soli Khajotia; Fernando Luis Esteban Florez; João César Zielak
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