Propriedades de transdiferenciação, osteocondução e osseoindução in vivo de biomateriais orgânicos experimentais solúveis em água – Estudo Piloto
DOI:
https://doi.org/10.33448/rsd-v10i5.15017Palavras-chave:
Engenharia de Tecidos; Orgânico; Biomateriais; Enxerto Ectópico; Regeneração Óssea; Imunohistoquímica.Resumo
A matriz óssea inorgânica bovina (IBBM) é um biomaterial com funcionalidades osteocondutoras comprovadas. Este estudo avaliou as funcionalidades ósseo-regenerativas do IBBM modificado ou não por MOE em ovinos. MOE foi sintetizado através da suspensão do nacre (0.05 g, diâmetros < 0.01 mm) em ácido acético anidro (pH 7, 25°C, 72 horas) usando agitação magnética. Tubos de polietileno (d = 5.00 mm, c = 10.0 mm, extremidades abertas) do controle negativo (sham) ou grupos experimentais (IBBM ou IBBM modificado por MOE) foram posicionados (n = 3/condição/animal; intramuscular) adjacentes á distal da coluna de ovelhas (8 animais, » 45 Kg, 2 anos). Tecidos foram colhidos (3 ou 6 meses) pós-implantação para análises histológicas (H), morfométricas (MM) e imunohistoquímicas (IH; Wnt-3a, CD34, Vimentin and PREF-1). Dados MM foram analisados utilizando os testes Shapiro-Wilk e Levene, Mann Whitney e Kruskal-Wallis. Dados IM foram analisados usando ANOVA de medidas repetidas e Tukey. Diferenças (p < 0.05) foram observadas entre of grupos experimentais (IBBM e IBBM + MOE aos 3 e 6 meses) e controles (sham) para área total; não foram encontradas diferenças para partículas remanescentes entre os grupos experimentais; osso neoformado ocorreu apenas na presença de biomateriais. Os maiores valores de osso neoformado foram observados com IBBM + MOE (6-meses). Diferenças estatísticas (p > 0.05) não encontradas na análise IM (Wnt-3a, CD34, Vimentin and PREF-1). Resultados indicam que materiais experimentais (IBBM + MOE) apresentam funcionalidades promissoras. Estudos adicionais são necessários para definir os efeitos longitudinais dos biomateriais e suas propriedades de biocompatibilidade de longo prazo.
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Copyright (c) 2021 Viviane Rozeira Crivellaro; Gilvan Spada; Cláudia Salete Judachesci ; Paula Porto Spada; Luiza Rodrigues Saling; Idélcena Tatiane Miranda; Tatiana Miranda Deliberador; Marilisa Carneiro Leão Gabardo; Rafaela Scariot; Moira Pedroso Leão; Carmen Lucia Mueller Storrer; Sharukh Soli Khajotia; Fernando Luis Esteban Florez; João César Zielak
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