Avaliação da suplementação com caseína sobre o periodonto e osso mandibular de ratos alimentados com dieta padrão e dieta de cafeteria

Marília Rizzi; Karoline Rodrigues  Pasqualotto; Gabrielle Delfrate; Tayline Mroczek; Amanda Priscilla  Soistak; Larissa Portela David; Marcela Claudino; Dionísia Xavier Scomparin

doi:10.33448/rsd-v10i7.16169

Autores/as

Marília Rizzi Universidade Estadual de Ponta Grossa https://orcid.org/0000-0002-6912-8446
Karoline Rodrigues Pasqualotto Universidade Estadual de Ponta Grossa https://orcid.org/0000-0001-8165-5540
Gabrielle Delfrate Delfrate https://orcid.org/0000-0002-4326-9566
Tayline Mroczek Universidade Estadual de Ponta Grossa https://orcid.org/0000-0003-1004-0789
Amanda Priscilla Soistak Universidade Estadual de Ponta Grossa https://orcid.org/0000-0002-1257-8884
Larissa Portela David Universidade Estadual de Ponta Grossa https://orcid.org/0000-0002-5555-3062
Marcela Claudino Universidade Estadual de Ponta Grossa https://orcid.org/0000-0003-1553-5852
Dionísia Xavier Scomparin Universidade Estadual de Ponta Grossa https://orcid.org/0000-0001-8658-2467

DOI:

https://doi.org/10.33448/rsd-v10i7.16169

Palabras clave:

Cafetería dietética; Proteína láctea; Caseína; Reabsorción ósea alveolar; Periodonto.

Resumen

Objetivo: Evaluar el efecto de la suplementación con caseína en el periodonto y el hueso mandibular de ratas alimentadas con una dieta estándar y una dieta de cafetería. Metodología: Se dividieron 24 ratas Wistar en cuatro grupos experimentales: dieta normal (DN), dieta normal con caseína (DNC), dieta de cafetería (DC) y dieta de cafetería con caseína (DCC). Los grupos DNC y DCC recibieron 2 mL de caseína micelar desde el día 60 al 90. A los 90 días, los animales se ortotanizaron y los materiales se recogieron para su análisis. Resultados: Los animales de los grupos DC y DCC mostraron un mayor índice de adiposidad en comparación con DN y DNC (p <0001). El grupo DCC tuvo un nivel de colesterol HDL significativamente menor que el DN (p <0,0092). En el análisis radiográfico de la hemimandibula de los animales, no se observó diferencia en relación a la densidad de píxeles, sin embargo, sí hubo diferencia en el tamaño de porción mandibular entre DCC y DNC (p <0.0196) y DCC y DN (p <0.0039). Histológicamente, hubo una reducción de los vasos sanguíneos, tanto en el hueso alveolar (p = 0,0033), hueso de furca (p <0,0001) como en el ligamento periodontal (p = 0,0001) en la EC. Además, en el hueso alveolar hubo una disminución de los osteocitos en el grupo DCC en relación con la CND (p = 0,0198). Conclusiones: El aumento de la adiposidad contribuyó a la disminución de vasos sanguíneos y osteocitos en el tejido óseo de ratas, a pesar de no influir en la inflamación. Este efecto fue independiente de la caseína. Por lo tanto, la suplementación con caseína en ratas sometidas a una dieta de cafetería no fue eficaz para disminuir la acumulación de tejido adiposo, ni tampoco interfirió positivamente en el metabolismo óseo del hemimandibula.

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Evaluación de la suplementación con caseína en periodonto y hueso mandibular de ratas alimentadas con una dieta estándar y una dieta de cafetería

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