Chemical composition, phytotoxicity and cytogenotoxicity of essential oil from leaves of Psidium guajava L. cultivars
DOI:
https://doi.org/10.33448/rsd-v10i9.17710Keywords:
Biological activity; Bioherbicides; Chromosome; Plant bioassay; Terpenes; Mitotic index.Abstract
Natural products with biological activity, such as essential oils, can be used in the search for and development of ecological herbicides as an alternative to reduce the damage caused by synthetic herbicides. This work to aimed to determine the chemical composition and phytotoxic properties of the essential oils, at concentrations of 3000, 1500, 750, 375 and 187.5 µg/mL, of four cultivars of Psidium guajava (guava) evaluated on germination and root growth of plant models Lactuca sativa and Sorghum bicolor, as well as in the L. sativa cell cycle. Exposure to essential oils reduced germination and root growth in bioassays, especially at the highest concentration (3000 µg/mL). The essential oils interfered in the normal dynamics of the cell cycle of L. sativa at most concentrations, causing a decrease in the mitotic index and increasing of chromosomal alterations, evidencing aneugenic and clastogenic action. The biological activity of the oils was associated with the presence of sesquiterpenes and monoterpenes found here, such as caryophyllene oxide, (E) -caryophyllene, and limonene. Thus, the essential oils of cultivars of guava demonstrated the promising potential for use as natural herbicides.
References
Adams, R. P. (2007). Identification of essential oil components by gas chromatography/mass spectrometry (Ed. 4). Allured Publishing Corporation.
Almeida, L., Teixeira, M. C., Lemos, J. R., Lacerda, M. N., & Silva, T. C. (2019). Bioatividade de óleos essenciais na germinação e no vigor em sementes de tomate. Biotemas, 32(2), 13–21.
Alves, T. de A., Pinheiro, P. F., Fontes, M. M. P., Andrade-Vieira, L. F., Corrêa, K. B., Alves, T. A., Cruz, F. A., Júnior, V. L., Ferreira, A., & Soares, T. C. B. (2018). Toxicity of thymol, carvacrol and their respective phenoxyacetic acids in Lactuca sativa and Sorghum bicolor. Industrial Crops and Products, 114, 59–67. https://doi.org/10.1016/j.indcrop.2018.01.071
Amri, I., Hamrouni, L., Hanana, M., Gargouri, S., Fezzani, T., & Jamoussi, B. (2013). Chemical composition, physico-chemical properties, antifungal and herbicidal activities of Pinus halepensis Miller essential oils. Biological Agriculture and Horticulture, 29(2), 91–106. https://doi.org/10.1080/01448765.2013.764486
Andrade-Vieira, L. F., Botelho, C. M., Palmieri, M.J., Laviola, B. G., & Praça-Fontes, M. M. (2014). Effects of Jatropha curcas oil in Lactuca sativa root tip bioassays. Anais da Academia Braseleira de Ciências, 86(1), 373-382. http://dx.doi.org/10.1590/0001-3765201420130041
Aragão, F. B., Queiroz, V. T., Ferreira, A., Costa, A. V., Pinheiro, P. F., Carrijo, T. T., & Andrade-Vieira, L. F. (2017). Phytotoxicity and cytotoxicity of Lepidaploa rufogrisea (Asteraceae) extracts in the plant model Lactuca sativa (Asteraceae). Revista de Biologia Tropical, 65(2) 1-10. https://doi.org/10.15517/rbt.v65i2.25696
Araniti, F., Graña, E., Krasuska, U., Bogatek, R., Reigosa, M. J., Abenavoli, M. R., & Sánchez-Moreiras, A. M. (2016). Loss of gravitropism in farnesene-treated arabidopsis is due to microtubule malformations related to hormonal and ROS unbalance. PLoS ONE, 11(8), 1–26. https://doi.org/10.1371/journal.pone.0160202
Aslam, F., Khaliq, A., Matloob, A., Tanveer, A., Hussain, S., & Zahir, Z. A. (2017). Allelopathy in agro-ecosystems: a critical review of wheat allelopathy-concepts and implications. Chemoecology, 27(1). https://doi.org/10.1007/s00049-016-0225-x
Babich, H. (1997). The “Allium” test: A simple, eukaryote genotoxicity assay. The American Biology Teacher, 59(9), 580–583. https://doi.org/10.2307/4450386
Barbosa, L. C. A., Demuner, A. J., Clemente, A. D., Paula, V. F., & Ismail, F. M. D. (2007). Seasonal variation in the composition of volatile oils from Schinus terebinthifolius Raddi. Quimica Nova, 30(8), 1959–1965. https://doi.org/10.1590/S0100-40422007000800030
Beech, E., Rivers, M., Oldfield, S., & Smith, P. P. (2017). GlobalTreeSearch: the first complete global database of tree species and country distributions. Journal of Sustainable Forestry, 36, 454–489. https://doi.org/10.1080/10549811.2017.1310049
Boaro, C. S. F., Vieira, M. A. R., Campos, F. G., Ferreira, G., Chacón, I. D. C., & Marques, M. O. M. (2019). Factors influencing the production and chemical composition of essential oils in aromatic plants from Brazil. Essential Oil Research, 19–47. https://doi.org/10.1007/978-3-030-16546-8_2
Caputo, L., Smeriglio, A., Trombetta, D., Cornara, L., Trevena, G., Valussi, M., Fratianni, F., Feo, V., & Nazzaro, F. (2020). Chemical composition and biological activities of the essential oils of Leptospermum petersonii and Eucalyptus gunnii. Frontiers in Microbiology, 11, 1–15. https://doi.org/10.3389/fmicb.2020.00409
Chaturvedi, T., Singh, S., Nishad, I., Kumar, A., Tiwari, N., Tandon, S., Saikia, D., & Verma, R. S. (2019). Chemical composition and antimicrobial activity of the essential oil of senescent leaves of guava (Psidium guajava L.). Natural Product Research, 35, 1393-1397. https://doi.org/10.1080/14786419.2019.1648462
Dayan, F. E., Cantrell, C. L., & Duke, S. O. (2009). Natural products in crop protection. Bioorganic and Medicinal Chemistry, 17(12), 4022–4034. https://doi.org/10.1016/j.bmc.2009.01.046
Dayan, F. E., & Duke, S. O. (2014). Natural compounds as next-generation herbicides. Plant Physiology, 166(3), 1090–1105. https://doi.org/10.1104/pp.114.239061
Dias, J. D. F. G., Miguel, O. G., & Miguel, M. D. (2009). Composition of essential oil and allelopathic activity of aromatic water of Aster lanceolatus Willd. (Asteraceae). Brazilian Journal of Pharmaceutical Sciences, 45(3), 469–474. https://doi.org/10.1590/S1984-82502009000300012
Duke, S. O., & Oliva, A. (2004). Mode of action of phytotoxic terpenoids. In H. Macias, F., Galindo, J., Molinillo, J., Cutler (Ed.), Allelopathy: Chemistry and mode of action of allelochemicals. CRC Press. https://doi.org/10.1201/9780203492789.ch10
Duke, Stephen O., Romagni, J. G., & Dayan, F. E. (2000). Natural products as sources for new mechanisms of herbicidal action. Crop Protection, 19, 583–589. https://doi.org/10.1016/S0261-2194(00)00076-4
Durazzini, A. M. S., Machado, C. H. M., Fernandes, C. C., Willrich, G. B., Crotti, A. E. M., Candido, A. C. B. B., Magalhães, L. G., Squarisi, I. S., Ribeiro, A. B., Tavares, D. C., Martins, C. H. G., & Miranda, M. L. D. (2019). Eugenia pyriformis Cambess: a species of the Myrtaceae family with bioactive essential oil. Natural Product Research. https://doi.org/10.1080/14786419.2019.1669031
El-Ghamery, A. A., El-Kholy, M. A., & Abou El-Yousser, M. A. (2003). Evaluation of cytological effects of Zn2+ in relation to germination and root growth of Nigella sativa L. and Triticum aestivum L. Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 537, 29-41. https://doi.org/10.1016/S1383-5718(03)00052-4
Fagodia, S. K., Singh, H. P., Batish, D. R., & Kohli, R. K. (2017). Phytotoxicity and cytotoxicity of Citrus aurantiifolia essential oil and its major constituents: Limonene and citral. Industrial Crops and Products, 108, 708–715. https://doi.org/10.1016/j.indcrop.2017.07.005
Fiskesjö, G. (1985). The Allium test as a standard in environmental monitoring. Hereditas, 102(1), 99–112. https://doi.org/10.1111/j.1601-5223.1985.tb00471.x
Giunti, G., Campolo, O., Laudani, F., Zappalà, L., & Palmeri, V. (2021). Bioactivity of essential oil-based nano-biopesticides toward Rhyzopertha dominica (Coleoptera: Bostrichidae). Industrial Crops and Products, 162, 113257. https://doi.org/10.1016/j.indcrop.2021.113257
Gruľová, D., Caputo, L., Elshafie, H. S., Baranová, B., De Martino, L., Sedlák, V., Gogaľová, Z., Poráčová, J., Camele, I., & Feo, V. (2020). Thymol chemotype Origanum vulgare L. essential oil as a potential selective bio-based herbicide on monocot plant species. Molecules, 25(3), 595. https://doi.org/10.3390/molecules25030595
Habermann, E., Pereira, V. D. C., Imatomi, M., Pontes, F. C. & Gualtieri, S. C. J. (2017). In vitro herbicide activity of crude and fractionated leaf extracts of Blepharocalyx salicifolius (Myrtaceae). Brazilian Journal of Botany, 40, 33-40. https://doi.org/10.1007/s40415-016-0317-4
Harashima, H., & Schnittger, A. (2010). The integration of cell division, growth and differentiation. Current Opinion in Plant Biology, 13(1), 66–74. https://doi.org/10.1016/j.pbi.2009.11.001
Harborne, J. B., & Tomas-Barberan, F. A. (1990). Ecological chemistry and biochemistry of plant terpenoids. Clarendon Press.
Hayes, W. B. (1953). Fruit Growing in India. Allahabad.
Hazrati, H., Saharkhiz, M. J., Niakousari, M., & Moein, M. (2017). Natural herbicide activity of Satureja hortensis L. essential oil nanoemulsion on the seed germination and morphophysiological features of two important weed species. Ecotoxicology and Environmental Safety, 142, 423–430. https://doi.org/10.1016/j.ecoenv.2017.04.041
Ibrahim, M., Farooq, T., Hussain, N., Hussain, A., Gulzar, T., Hussain, I., Akash, M. S., & Rehmani, F. S. (2013). Acetyl and butyryl cholinesterase inhibitory sesquiterpene lactones from Amberboa ramosa. Chemistry Central Journal, 7(1), 3–7. https://doi.org/10.1186/1752-153X-7-116
Inderjit, Wardle, D. A., Karban, R., & Callaway, R. M. (2011). The ecosystem and evolutionary contexts of allelopathy. Trends in Ecology and Evolution, 26, 655-662. https://doi.org/10.1016/j.tree.2011.08.003
Jabran, K., Mahajan, G., Sardana, V., & Chauhan, B. S. (2015). Allelopathy for weed control in agricultural systems. Crop Protection, 72, 57–65. https://doi.org/10.1016/j.cropro.2015.03.004
Jana, A., & Biswas, S. M. (2011). Lactam nonanic acid, a new substance from Cleome viscosa with allelopathic and antimicrobial properties. Journal of Biosciences, 36(1), 27–35. https://doi.org/10.1007/s12038-011-9001-9
Jerônimo, L. B., da Costa, J. S., Pinto, L. C., Montenegro, R. C., Setzer, W. N., Mourão, R. H. V., da Silva, J. K. R., Maia, J. G. S., & Figueiredo, P. L. B. (2021). Antioxidant and cytotoxic activities of Myrtaceae essential oils rich in terpenoids from Brazil. Natural Product Communications, 16(2), 1934578X2199615. https://doi.org/10.1177/1934578X21996156
Khadhri, A., El Mokni, R., Almeida, C., Nogueira, J. M. F., & Araújo, M. E. M. (2014). Chemical composition of essential oil of Psidium guajava L. growing in Tunisia. Industrial Crops and Products, 52, 29–31. https://doi.org/10.1016/j.indcrop.2013.10.018
Lee, W. C., Mahmud, R., Noordin, R., Pillai Piaru, S., Perumal, S., & Ismail, S. (2013). Free radicals scavenging activity, cytotoxicity and anti-parasitic activity of essential oil of Psidium guajava L. leaves against Toxoplasma gondii. Journal of Essential Oil-Bearing Plants, 16(1), 32–38. https://doi.org/10.1080/0972060X.2013.764196
Leme, D. M., & Marin-Morales, M. A. (2009). Allium cepa test in environmental monitoring: A review on its application. Mutation Research - Reviews in Mutation Research, 682, 71-81. https://doi.org/10.1016/j.mrrev.2009.06.002
Nist standard reference database 69. (2011). NIST Chemistry WebBook. https://webbook.nist.gov/chemistry.
Maffei, M. E., Gertsch, J., & Appendino, G. (2011). Plant volatiles: production, function and pharmacology. Natural Product Reports, 28(8), 1359–1380. https://doi.org/10.1039/c1np00021g
Matoba, H., Mizutani, T., Nagano, K., Hoshi, Y., & Uchiyama, H. (2007). Chromosomal study of lettuce and its allied species (Lactuca spp., Asteraceae) by means of karyotype analysis and fluorescence in situ hybridization. Hereditas, 144(6), 235–243. https://doi.org/10.1111/j.2007.0018-0661.02012x
Matsumoto, S. T., Mantovani, M. S., Malaguttii, M. I. A., Dias, A. L., Fonseca, I. C., & Marin-Morales, M. A. (2006). Genotoxicity and mutagenicity of water contaminated with tannery effluents as evaluated by the micronucleus test and comet assay using the fish Oreochromis niloticus and chromosome aberrations in onion root-tips. Genetics and Molecular Biology, 29, 148–158. https://doi.org/10.1590/S1415-47572006000100028
Mendes, L. A., Martins, G. F., Valbon, W. R., Souza, T. S., Menini, L., Ferreira, A., & Ferreira, M. F. S. (2017). Larvicidal effect of essential oils from Brazilian cultivars of guava on Aedes aegypti L. Industrial Crops and Products, 108, 684–689. https://doi.org/10.1016/j.indcrop.2017.07.034
Navarro-Rocha, J., Andrés, M. F., Díaz, C. E., Burillo, J., & González-Coloma, A. (2020). Composition and biocidal properties of essential oil from pre-domesticated spanish Satureja montana. Industrial Crops and Products, 145, 111958. https://doi.org/10.1016/j.indcrop.2019.111958
Oliveira Jr., R. S. (2011). Mecanismos de Ação de Herbicidas in Biologia e Manejo de plantas Daninhas. Omnipax.
Pinheiro, P. F., Costa, A. V., Alves, T. A., Galter, I. N., Pinheiro, C. A., Pereira, A. F., Oliveira, C. M. R., & Fontes, M. M. P. (2015). Phytotoxicity and cytotoxicity of essential oil from leaves of Plectranthus amboinicus, carvacrol, and thymol in plant bioassays. Journal of Agricultural and Food Chemistry, 63(41), 8981–8990. https://doi.org/10.1021/acs.jafc.5b03049
R Development Core Team. (2020). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. http://www.R-project.org/
Rajkumar, V., Gunasekaran, C., Paul, C. A., & Dharmaraj, J. (2020). Development of encapsulated peppermint essential oil in chitosan nanoparticles: characterization and biological efficacy against stored-grain pest control. Pesticide Biochemistry and Physiology, 170, 104679. https://doi.org/10.1016/j.pestbp.2020.104679
Ribas, P. P., & Matsumura, A. T. S. (2009). A química dos agrotóxicos: impacto sobre a saúde e meio ambiente. Revista Liberato, 10(14), 149–158. https://doi.org/10.31514/rliberato.2009v10n14.p149
Rice, E. L. (2012). Allelopathy. Academic press.
Ruilong, W., Shaolin, P., Rensen, Z., Ling, W. D., & Zengfu, X. U. (2009). Cloning, expression and wounding induction of β-caryophyllene synthase gene from Mikania micrantha H.B.K. and allelopathic potential of β-caryophyllene. Allelopathy Journal, 24(1), 35–44.
Satyal, P., Paudel, P., Lamichhane, B., & Setzer, W. N. (2015). Leaf essential oil composition and bioactivity of Psidium guajava from Kathmandu, Nepal. American Journal of Essential Oils and Natural Products, 3(2), 11–14.
Sousa, S. M., Silva, P. S., Campos, J. M. S., & Viccini, L. F. (2009). Cytotoxic and genotoxic effects of two medicinal species of Verbenaceae. Caryologia, 62(4), 326-333 https://doi.org/10.1080/00087114.2004.10589698
Scalvenzi, L., Grandini, A., Spagnoletti, A., Tacchini, M., Neill, D., Ballesteros, J., Sacchetti, G., & Guerrini, A. (2017). Myrcia splendens (Sw.) DC. (syn. M. fallax (Rich.) DC.) (Myrtaceae) essential oil from Amazonian Ecuador: a chemical characterization and bioactivity profile. Molecules, 22(7), 1163. https://doi.org/10.3390/molecules22071163
Sharifi-Rad, J., Sureda, A., Tenore, G. C., Daglia, M., Sharifi-Rad, M., Valussi, M., Tundis, R., Sharifi-Rad, M., Loizzo, M. R., Ademiluyi, A. O., Sharifi-Rad, R., Ayatollahi, S. A., & Iriti, M. (2017). Biological activities of essential oils: From plant chemoecology to traditional healing systems. Molecules, 22, 70. https://doi.org/10.3390/molecules22010070
Shu, J. C., Liu, J. Q., Chou, G. X., & Wang, Z. T. (2012). Two new triterpenoids from Psidium guajava. Chinese Chemical Letters, 23(7), 827–830. https://doi.org/10.1016/j.cclet.2012.05.018
Silveira, G. L., Lima, M. G. F., Dos Reis, G. B., Palmieri, M. J. & Andrade-Vieira, L. F. (2017). Toxic effects of environmental pollutants: Comparative investigation using Allium cepa L. and Lactuca sativa L. Chemosphere, 178, 359–367. https://doi.org/10.1016/j.chemosphere.2017.03.048
Soliman, F. M., Fathy, M. M., Salama, M. M., & Saber, F. R. (2016). Comparative study of the volatile oil content and antimicrobial activity of Psidium guajava L. and Psidium cattleianum Sabine leaves. Bulletin of Faculty of Pharmacy, Cairo University, 54(2), 219–225. https://doi.org/10.1016/j.bfopcu.2016.06.003
Souza, T. S., Ferreira, Ferreira, M. S. F., Menini, L., Souza, J. R. C. L., Parreira, L. A., Cecon, P. R., & Ferreira, A. (2017). Essential oil of Psidium guajava: Influence of genotypes and environment. Scientia Horticulturae, 216, 38–44. https://doi.org/10.1016/j.scienta.2016.12.026
Taban, A., Saharkhiz, M. J., & Naderi, R. (2020). A natural post-emergence herbicide based on essential oil encapsulation by cross-linked biopolymers: characterization and herbicidal activity. Environmental Science and Pollution Research, 27(36), 45844–45858. https://doi.org/10.1007/s11356-020-10405-y
Vasconcelos, L. C., Santos, E. S., Bernardes, C. O., Ferreira, M. F. S., Ferreira, A., Tuler, A. C., Carvalho, J. A. M., Pinheiro, P. F., & Praça-Fontes, M. M. (2019). Phytochemical analysis and effect of the essential oil of Psidium L. species on the initial development and mitotic activity of plants. Environmental Science and Pollution Research, 26, 26216-26228. https://doi.org/10.1007/s11356-019-05912-6
Vaughn, S. F., & Spencer, G. F. (1993). Volatile monoterpenes as potential parent structures for new herbicides. Weed Science, 41, 114-119. https://doi.org/10.1017/s0043174500057672
Vokou, D., Douvli, P., Blionis, G. J., & Halley, J. M. (2003). Effects of monoterpenoids, acting alone or in pairs, on seed germination and subsequent seedling growth. Journal of Chemical Ecology, 29(10), 2281–2301. https://doi.org/10.1023/A:1026274430898
Weli, A., Al-Kaabi, A., Al-Sabahi, J., Said, S., Hossain, M. A., & Al-Riyami, S. (2019). Chemical composition and biological activities of the essential oils of Psidium guajava leaf. Journal of King Saud University - Science, 31(4), 993–998. https://doi.org/10.1016/j.jksus.2018.07.021
Wilson, P. G. (2010). Myrtaceae in Flowering Plants. Eudicots. The Families and Genera of Vascular Plants. Springer.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Loren Cristina Vasconcelos; Esdras de Souza Santos; Luiza Alves Mendes; Marcia Flores da Silva Ferreira; Milene Miranda Praça-Fontes
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
