Análisis de las proteínas salivales de los corredores de la calle después de un evento deportivo real
DOI:
https://doi.org/10.33448/rsd-v10i10.18183Palabras clave:
Proteómica; Espectrometria de Masas; Ejercicio; Saliva.Resumen
El objetivo fue caracterizar las cambios inducidos por el ejercicio físico agudo en las proteínas salivales de los corredores callejeros. Se recogieron muestras de saliva de 12 atletas masculinos adultos antes e inmediatamente después de una carrera de calle. Se crearon dos grupos en función de la distancia recorrida, 5 km (n = 4) y 10 km (n = 8). Las muestras fueron sometidas a agotamiento de amilasa, albúmina e inmunoglobulina G. Luego fueron concentradas, digeridas y analizadas con nano-UPLC tándem nano-ESI-MSE. En total se identificaron 69 proteínas. Se observaron cambios significativos en la expresión de 15 proteínas en el grupo de 5 km y 13 proteínas en el grupo de 10 km. Entre las proteínas con expresión alterada, solo 7 ya habían sido descritas en la literatura en modelos similares (Alfa Amilasa 1, Lactoperoxidasa, Alfa Actina del Músculo Esquelético, Cistatina-B, Cistatina-SN, Cistatina-SA y Proteína 3B regulada por andrógenos en glándula submaxilar). Este estudio demostró que correr en la calle promueve cambios agudos en el proteoma salival, los resultados obtenidos se unen a los pocos datos disponibles en la literatura en la búsqueda de una mejor comprensión de los efectos agudos del ejercicio.
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Derechos de autor 2021 Cícero Matheus Lima Amaral; Iago Almeida da Ponte; Géssica de Souza Martins; Daniel Freire Lima; Abelardo Barbosa Moreira Lima Neto; Ana Cristina Monteiro Moreira; Maria Izabel Florindo Guedes
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