Influence of the extraction process on the antioxidant capacity of pequi pulp extracts

Flávia de Abreu  Pinheiro; Leonardo Luiz  Okumura; Astréa Filomena de Souza  Silva; Júnio Gonçalves da  Silva; Deusélio Bassini  Fioresi; Wilton Soares  Cardoso; Letícia Rocha  Ferreira; Edimar Aparecida Filomeno Fontes

doi:10.33448/rsd-v10i12.20663

Autores/as

Flávia de Abreu Pinheiro Federal Institute of Espírito Santo https://orcid.org/0000-0002-4310-0592
Leonardo Luiz Okumura Federal University of Vicosa https://orcid.org/0000-0002-3056-2966
Astréa Filomena de Souza Silva Federal University of Vicosa https://orcid.org/0000-0003-1992-7968
Júnio Gonçalves da Silva Federal University of Minas Gerais https://orcid.org/0000-0002-3714-3633
Deusélio Bassini Fioresi Federal Institute of Espírito Santo https://orcid.org/0000-0002-0912-0813
Wilton Soares Cardoso Federal Institute of Espírito Santo https://orcid.org/0000-0001-8067-6921
Letícia Rocha Ferreira Federal University of Vicosa https://orcid.org/0000-0003-0983-5584
Edimar Aparecida Filomeno Fontes Federal University of Vicosa https://orcid.org/0000-0002-4370-8805

DOI:

https://doi.org/10.33448/rsd-v10i12.20663

Palabras clave:

Disolvente; Compuesto bioactivo; Análisis voltamétrico; Ensayo electroquímico; Voltametría de pulso diferencial; Análisis de componentes principales.

Resumen

Se investigó el efecto del procedimiento de extracción sobre la capacidad antioxidante de los extractos de pulpa de pequi: acuoso (AQ), alcohólico (ALC) y acetometilo (AM), obtenidos respectivamente por los disolventes agua, etanol y metanol seguido de acetona. La acción antioxidante se expresó mediante el coeficiente de capacidad antioxidante (K) obtenido por la prueba electroquímica (DPV) a diferentes valores de pH, y en equivalente de trolox (TEAC), por DPV (pH 7,15) y ensayos espectrofotométricos ABTS y DPPH. También se determinaron los contenidos totales de carotenoides y fenólicos. Mediante análisis de Componentes Principales y Cluster, se observó una mayor similitud de K entre AM, trolox y ácido gálico, AQ y ácido L-ascórbico, y ALC y β-caroteno, independientemente del pH. A través del análisis de Cluster, la mayor diferenciación de K fue a pH 2,20. En general, la AM muestra una mejor acción antioxidante (TEAC). AQ y ALC mostraron el mayor contenido de fenólicos y carotenoides, respectivamente. El método de extracción influyó en el contenido de compuestos bioactivos en los extractos de pequi y, por tanto, en su capacidad antioxidante.

Citas

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Influencia del proceso de extracción en la capacidad antioxidante de los extractos de pulpa de pequi

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