Diferentes estrategias para aumentar la producción de biosurfactantes de un aislado de Paenibacillus sp. (BR13834)

Autores/as

DOI:

https://doi.org/10.33448/rsd-v10i17.24232

Palabras clave:

DCCR; Biosurfactante; Paenibacillus sp.

Resumen

Los biosurfactantes son metabolitos producidos por varios microorganismos, y en los últimos años están despertando el interés de la comunidad científica debido a sus ventajas sobre los surfactantes sintéticos. El presente estudio tuvo como objetivo aumentar la producción de biosurfactante por Paenibacillus sp., optimizando los factores de crecimiento fuente de carbono, pH, temperatura y tiempo de cultivo. Inicialmente, se evaluaron las fuentes de carbono glucosa, lactosa, aceite de oliva, aceite de soja, glicerina y parafina. A continuación, se evaluaron los factores pH, temperatura y tiempo, mediante un diseño factorial para identificar los factores que influyen en el proceso de producción de biosurfactantes. Finalmente, se evaluaron los factores ambientales temperatura y tiempo de cultivo mediante un diseño rotacional compuesto central (DCCR). En todos los ensayos se utilizó el aislado BR13834 perteneciente al género Paenibacillus. El modelo empírico de predicción desarrollado resultó adecuado para describir la producción de biosurfactantes en relación con la tensión superficial (R2 = 0,755). El valor mínimo de la tensión superficial fue de 34,6 mN/m, obtenido en las condiciones ideales de 30°C y 24 horas de cultivo. Los resultados demostraron que el DCCR era adecuado para identificar las mejores condiciones para la producción del biosurfactante producido por Paenibacillus sp.

Biografía del autor/a

Elisa Maria de Oliveira, Universidade do Estado do Amapá

Universidade do Estado do Amapá. Av. Presidente Vargas, 650, Central, 68900-070, Macapá, AP, Brasil.

 Programa de pós-graduação Biodiversidade e Biotecnologia da Rede Bionorte (PPG-Bionorte)

Victor Hugo Gomes Sales, Instituto Federal do Amapá

Instituto Federal do Amapá, BR 210, km 03, Brasil Novo, 68909-398, Macapá, AP, Brasil.

Elora Dannan Corrêa Dias, Universidade do Estado do Amapá

Universidade do Estado do Amapá. Av. Presidente Vargas, 650, Central, 68900-070, Macapá, AP, Brasil.

Wardsson Lustrino Borges, Embrapa Amapá, Macapá, AP; Embrapa Agroindústria Tropical, Fortaleza, CE

Embrapa Amapá, Rodovia Juscelino Kubitscheck, km 5, Universidade, CEP 68903-419, Macapá, AP, Brasil.

Embrapa Agroindústria Tropical, Rua Doutora Sara Mesquita, 2270 Pici, CEP 60511-110 Fortaleza, CE, Brasil.

Marcelo Silva Andrade, Universidade do Estado do Amapá

Universidade do Estado do Amapá. Av. Presidente Vargas, 650, Central, 68900-070, Macapá-AP, Brasil.

Tiago Marcolino de Souza , Universidade do Estado do Amapá

Universidade do Estado do Amapá. Av. Presidente Vargas, 650, Central, 68900-070, Macapá-AP, Brasil.

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Publicado

20/12/2021

Cómo citar

OLIVEIRA, E. M. de; SALES, V. H. G.; DIAS, E. D. C.; BORGES, W. L.; ANDRADE, M. S. .; SOUZA , T. M. de . Diferentes estrategias para aumentar la producción de biosurfactantes de un aislado de Paenibacillus sp. (BR13834). Research, Society and Development, [S. l.], v. 10, n. 17, p. e44101724232, 2021. DOI: 10.33448/rsd-v10i17.24232. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/24232. Acesso em: 23 nov. 2024.

Número

Sección

Ciencias Agrarias y Biológicas