Cambios en la superficie del esmalte después del uso de flúor nanoencapsulado para la remineralización de caries dental: un estudio in vitro

Autores/as

DOI:

https://doi.org/10.33448/rsd-v11i4.26176

Palabras clave:

Remineralización dental; Caries dental; Pasta de dientes; Flúor; Nanotecnología.

Resumen

Objetivo: Evaluar por medio de un estudio in vitro los cambios en la superficie del esmalte cariado tras el uso de dentífricos fluorados con tecnología nanoencapsulada (NanoF). Métodos: Se distribuyeron 40 bloques de esmalte humano en cuatro grupos (n = 10): 50% NanoF + 50% NaF (50% nF), 100% NanoF (100% nF), 100% NaF como control positivo (CP) y sin flúor como control negativo (CN). Las muestras se sometieron a un modelo de ciclos de pH durante 7 días. Se midieron la microdureza superficial (SMH), la rugosidad superficial lineal (Ra) y la rugosidad de área superficial (Sa) antes y después de la formación de la lesión cariada y al final del tratamiento. Se calcularon los porcentajes de recuperación de la microdureza superficial (%SMHR), Ra alterada (%RaC) y Sa alterada (%SaC). Los datos se analizaron mediante la prueba ANOVA, ANOVA de medidas repetidas y la prueba de correlación de Pearson (p<0,05). Resultados: No se encontraron diferencias significativas entre los grupos para Ra, Sa, %RaC o %SaC (p>0,05). Ra y Sa aumentaron después del tratamiento. Se encontró una correlación significativa entre Ra y Sa. El CP tuvo el mayor %SMHR, seguido del 100% nF (p<0,05). Por el contrario, no se encontró remineralización superficial en los grupos de 50% nF y CN. Conclusión: Se encontraron cambios en la superficie del esmalte tras el tratamiento remineralizador con el grupo de 100% nF. La mayoría de los dentífricos con flúor proporcionaron un aumento de la rugosidad y la microdureza superficial. La nanotecnología es un método innovador y prometedor para la liberación controlada de flúor y en la remineralización de dientes con caries dental.

Biografía del autor/a

Juliellen Luiz da Cunha, Federal University of Paraíba

Undergraduate Dentistry Course, Health Sciences Centre, Federal University of Paraíba - UFPB, João Pessoa/Paraíba, Brazil.

Elizabeth Barreto Galvão de Sousa, Federal University of Paraíba

Undergraduate Dentistry Course, Health Sciences Centre, Federal University of Paraíba - UFPB, João Pessoa/Paraíba, Brazil.

Nayanna Lana Soares Fernandes, Federal University of Paraíba

Department of Clinical and Social Dentistry, Federal University of Paraíba - UFPB, João Pessoa/Paraíba, Brazil.

Ingrid Andrade Meira, University of Campinas

Department of Prosthodontics and Periodontology, Piracicaba Dental School, University of Campinas- UNICAMP, Piracicaba/São Paulo, Brazil.

Thiago Isidro Vieira, Federal University of Paraíba

Department of Morphology, Federal University of Paraíba - UFPB, João Pessoa/Paraíba, Brazil.

Ana Maria Barros Chaves Pereira, Federal University of Paraíba

Department of Morphology, Federal University of Paraíba - UFPB, João Pessoa/Paraíba, Brazil

Fabio Correia Sampaio, Federal University of Paraíba

Department of Clinic and Social Dentistry, Federal University of Paraiba - UFPB, João Pessoa/Paraiba, Brazil.

Andressa Feitosa Bezerra de Oliveira, Federal University of Paraíba

Department of Morphology, Federal University of Paraíba - UFPB, João Pessoa/Paraíba, Brazil.

Citas

Ahmadian, E., Shani, S., Yazdani, J., Dizaj, S. M., & Shariji, S. (2018). Local treatment of the dental caries using nanomaterials. Biomedicine & Pharmacotherapy, 108, 443-447. https://doi.org/10.1016/j.biopha.2018.09.026.

Alexandria, A. K., Meckelburg, N. A., Puetter, U. T., Salles, J. T., Souza, I. P. R., & Maia, L. C. (2016). Do pediatric medicines induce topographic changes in dental enamel? Brazilian Oral Research, 30:S1806-83242016000100211. https://doi.org/10.1590/1807-3107BOR-2016.vol30.0011.

Alves, V. F., Moreira, V. G., Soares, A. F., Albuquerque, L. S., Moura, H. S., Silva, A. O., & Sampaio, F. C. (2018). A randomized triple-blind crossover trial of a hydrocolloid-containing dentifrice as a controlled-release system for fluoride. Clinical Oral Investigations, 22(9), 3071-3077. https://doi.org/10.1007/s00784-018-2395-0.

Amaechi, B. T. (2019). Protocols to study dental caries in vitro: pH cycling models. In: Walker J (editor), Odontogenesis: Methods and Protocols (379-392). Methods in Molecular Biology. https://doi.org/10.1007/978-1-4939-9012-2_34

Ando, M., Fontana, M., Eckert, G. J., Arthur, R. A., Zhang, H., & Zero, D. T. (2018a). Objective and quantitative assessment of caries lesion activity. Journal of Dentistry, 78, 76-82. https://doi.org/10.1016/j.jdent.2018.08.009.

Ando, M., Shaikh, S., & Eckert, G. (2018b). Determination of Caries Lesion Activity: Reflection and Roughness for Characterization of Caries Progression. Operative Dentistry, 43(3), 301-306. https://doi.org/10.2341/16-236-L.

Buzalaf, M. A. R., Hannas, A. R., Magalhães, A. C., Rios, D., Honório, H. M., & Delbem, A. C. (2010). pH-cycling models for in vitro evaluation of the efficacy of fluoridated dentifrices for caries control: strengths and limitations. Journal of Applied Oral Science, 18(4), 316–334. https://doi.org/10.1590/S1678-77572010000400002.

Cardoso, C. A. B., Mangueira, D. F. B., Olympio, K. P. K., Magalhães, A. C., Rios, D., Honório, H. M. & Buzalaf, M. A. R. (2014). The effect of pH and fluoride concentration of liquid dentifrices on caries progression. Clinical Oral Investigations, 18(3), 761-767. https://doi.org/10.1007/s00784-013-1031-2.

Cate, J. M. T., & Buzalaf, M. A. R. (2019). Fluoride Mode of Action: Once There Was an Observant Dentist. Journal of Dental Research, 98(7), 725-730. https://doi.org/10.1177/0022034519831604.

Farooq, I., & Bugshan, A. (2020). The role of salivary contents and modern technologies in the remineralization of dental enamel: a narrative review. F1000 Research, 9, 171. https://doi.org/10.12688/f1000research.22499.3.

Gonzáles-cabezas, C., & Fernandez, C. E. (2018). Recent advances in remineralization therapies for caries lesions. Advances in Dental Research, 29(1), 55-59. https://doi.org/10.1177/0022034517740124.

Kanduti, D., Sterbenk, P., & Artnik, B. (2016). Fluoride: a review of use and effects on health. Materia Socio-medica, 28(2), 133-7. https://doi.org/10.5455/msm.2016.28.133-137.

Lavôr, J. R., Fernandes, N. L. S., de Sousa, E. B. G., da Cunha, J. L., Meira, I. A., Sampaio, F. C., & Oliveira, A. F. B. (2020). Nanoencapsulated fluoride as a remineralization option for dental erosion: an in vitro study. Acta Odontologica Scandinavica, 79(5), 321-326. https://doi.org/10.1080/00016357.2020.1849793

Lippert, F., & Gill, K. K. (2019). Carious lesion remineralizing potential of fluoride- and calcium-containing toothpastes. Journal of the American Dental Association, 150(5), 345-351. https://doi.org/10.1016/j.adaj.2018.11.022.

Ma, X., Lin, X., Zhong, T., & Xie, F. (2019). Evaluation of the efficacy of casein phosphopeptide-amorphous calcium phosphate on remineralization of white spot lesions in vitro and clinical research: a systematic review and meta-analysis. BMC Oral Health, 19(1), 295. https://doi.org/10.1186/s12903-019-0977-0.

Moreira, V. G. (2017). Clinical trial of bioavailability of fluoride in controlled release system dentifrices. [Master’s Thesis]. https://sig-arq.ufpb.br/arquivos/2017209023f1f95260044bf31313ab6a/Vanderlcia_Gomes_Moreira_DISSERTAO.pdf . Accessed 15 Mar 2021.

Nguyen, S., & Hiorth, M. (2015). Advanced Drug Delivery Systems for Local Treatment of the Oral Cavity. Therapeutic Delivery, 6(5), 595-608.https://doi.org/10.4155/tde.15.5

Nguyen, S., Escudero, C., Sediqi, N., Smistad, G., & Hiorth, M. (2017). Fluoride loaded polymeric nanopacticles for dental delivery. European Journal of Pharmaceutical Sciences, 104, 326-334. https://doi.org/10.1016/j.ejps.2017.04.004.

Oliveira, A. F. B., Sousa, E. B. G., Fernandes, N. L. S., Meira, I. A., Lavôr, J. R., Chaves, A. M. B. P., & Sampaio, F. C. (2021). Effect of treatment time on performance of nano-encapsulated fluoride dentifrices for remineralization of initial carious lesions: an in vitro study. Acta Odontologica Latinoamericana. 34(1), 56-62.

Paula, A. B. P., Fernandes, A. R., Coelho, A. S., Marto, C. M., Ferreira, M. M., Caramelo, F. & Carrilho, E. (2017). Therapies for White spot lesions- a systematic review. The Journal of Evidence-Based Dental Practice, 17(1), 23-38. https://doi.org/10.1016/j.jebdp.2016.10.003

Piñón-Segundo, E., Mendoza Muñoz, N., & Quintanar, D. (2019). Nanoparticles as Dental Drug-Delivery Systems. In K. Subramani, & W. Ahmed (Eds.), Nanobiomaterials in Clinical Dentistry (pp.567-593). https://doi.org/10.1016/B978-0-12-815886-9.00023-1

Pitts, N. B., Zero, D. T., Marsh, P. D., Ekstrand, K., Weintraub, J. A., Ramos-gomez, F. & Ismail, A. (2017). Dental caries. Nature Reviews, 3:17030. doi: 10.1038/nrdp.2017.30.

Queiroz, C. S., Hara, A. T., Paes Leme, A. F., & Cury, J. A. (2008). pH-Cycling models to evaluate the effect of low fluoride dentifrice on enamel De- and remineralization. Brazilian Dental Journal, 19(1), 21-7. https://doi.org/10.1590/S0103-64402008000100004.

Reis, P. Q. R., Calazans, F. S., Poubel, L. A., Silva, E. M., Alves, W. V., & Barceleiro, M. O. (2017). Avaliação in vitro do efeito de um dentifrício à base de nanohidroxiapatita na rugosidade de superfície do esmalte dental bovino. Revista Brasileira de Odontologia, 74(2), 133-137. https://doi.org/10.18363/rbo.v74n2.p.133

Sampaio, F., Fernandes, N. L., Sousa, E., Costa, J. G. S., Elbert, J. J., & Oliveira, A. (2020). Silicate-based Dentifrices Against Enamel Demineralization: In vitro Study [Conference presentation]. https://iadr.abstractarchives.com/abstract/20iags-3321441/silicate-based-dentifrices-against-enamel-demineralization-in-vitro-study.

Sleibi, A., Tappuni, A. R., Davis, G. R., Anderson, P., & Baysan, A. (2018). Comparison of efficacy of dental varnish containing fluoride either with CPP-ACP or bioglass on root caries: Ex vivo study. Journal of Dentistry, 73, 91-96.https://doi.org/10.1016/j.jdent.2018.04.009.

Soares, I. A., Leite, P. K. B. S., Farias, O. R., Lemos, G. A., Batista, A. U. D., & Montenegro, R. V. (2019). Polishing Methods Influence on Color Stability and Roughness of 2 Provisional Prosthodontic Materials. Journal of Prosthodontics, 28(5), 564-571. https://doi.org/10.1111/jopr.13062.

Tenuta, L. M. A., & Cury, J. A. (2013). Laboratory and human studies to estimate anticaries efficacy of fluoride toothpastes. Monographs in Oral Science, 23, 108–124 https://doi.org/10.1159/000350479.

Tomaz, P. L. S, Sousa, L. A., Aguiar, K. F., Oliveira, T. S., Matochek, M. H. M., Polassi, M. R., & D’Alpino, P. H. P., (2020). Effects of 1450-ppm Fluoride-cointaning toothpastes associated with boosters on the enamel remineralization and surface roughness after cariogenic challenge. European Journal of Dentistry, 14(1), 161-170. doi: 10.1055/s-0040-1705072.

Vieira, A., Delbem, A. C. B., Sassaki, K. T., Rodrigues, E., Cury, J. A., & Cunha, R. F. (2005). Fluoride dose response in pH-cycling models using bovine enamel. Caries Research, 39(6), 514-20.https://doi.org/10.1159/000088189.

Vyavhare, S., Sharma, D., & Kulkarni, V. (2015). Effect of Three Different Pastes on Remineralization of Initial Enamel Lesion: In Vitro Study. Journal of Clinical Pediatric Dentistry, 39(2), 149-60.https://doi.org/10.17796/jcpd.39.2.yn2r54nw24l03741.

Yu, O. Y., Zhao, I. S., Mei, M. L., Lo, E. C., & Chu, C. H. (2017). A Review of the Common Models Used in Mechanistic Studies on Demineralization-Remineralization for Cariology Research. Dentistry Journal (Basel), 5(2): 20.https://doi.org/10.3390/dj5020020.

Descargas

Publicado

27/03/2022

Cómo citar

CUNHA, J. L. da .; SOUSA, E. B. G. de .; FERNANDES, N. L. S.; MEIRA, I. A.; LAVÔR, J. R. de; VIEIRA, T. I.; PEREIRA, A. M. B. C.; SAMPAIO, F. C. .; OLIVEIRA, A. F. B. de . Cambios en la superficie del esmalte después del uso de flúor nanoencapsulado para la remineralización de caries dental: un estudio in vitro. Research, Society and Development, [S. l.], v. 11, n. 4, p. e7611426176, 2022. DOI: 10.33448/rsd-v11i4.26176. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/26176. Acesso em: 30 jun. 2024.

Número

Sección

Ciencias de la salud