Estrés calórico en caña de azúcar: cambios fisiológicos y expresión génica
DOI:
https://doi.org/10.33448/rsd-v11i3.26260Palabras clave:
OJIP; Clorofila; Fluorescencia; ARN; Fotosistema.Resumen
Ante el cambio climático, es necesario conocer los efectos de la temperatura en el desarrollo de las plantas, e identificar qué factores fisiológicos están relacionados con la reducción y/o aumento de la fotosíntesis. Esta identificación puede hacerse en las primeras etapas de desarrollo. Así, el objetivo fue evaluar parámetros fisiológicos del cultivar de caña de azúcar RB 86 7515 a 15°C, 28°C y 40°C a diferentes tiempos de exposición (0, 4, 8, 12 y 24 horas) y evaluar la expresión del gen 18S. El experimento se estableció en un diseño completamente al azar, con seis repeticiones. Se midieron clorofila a y b, y se estimó la relación a/b, clorofila total en hojas y variables relacionadas con los fotosistemas. Se midieron los valores de fluorescencia inicial (F0) y máxima (Fm). Con estas variables en mente, se estimaron las relaciones Fv/Fo y la eficiencia cuántica potencial del fotosistema II (PSII) Fv/Fm. El ARNm se extrajo usando los mismos tipos de hojas que se usaron en la evaluación de la fotosíntesis. Las altas temperaturas con exposición durante 24 horas afectan la eficiencia fotosintética de las plantas de caña de azúcar. Se recomienda la exposición de plantas de caña de azúcar con 65 días de desarrollo a 40ºC por 24 horas para evaluar la tolerancia al estrés por temperatura, visando la selección temprana de genotipos.
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Derechos de autor 2022 Airan Miguel dos Santos Panta; Juliana Lopes Souza; Paulo Roberto Gagliardi; Luiz Fernando Ganassali Oliveira Junior; Pryanka Thuyra Nascimento Fontes; Jailson Lara Fagundes; Renata Silva-Mann
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