Estresse térmico em cana-de-açúcar: mudanças fisiológicas e expressão gênica
DOI:
https://doi.org/10.33448/rsd-v11i3.26260Palavras-chave:
OJIP; Clorofila; Fluorescência; RNA; Fotossistema.Resumo
Frente às mudanças climáticas faz-se necessário conhecer os efeitos da temperatura no desenvolvimento das plantas, e identificar quais fatores fisiológicos estão relacionados à redução e/ou aumento da fotossíntese. Esta identificação pode ser feita nos primeiros estádios de desenvolvimento. Assim, objetivou-se avaliar parâmetros fisiológicos da cultivar de cana-de-açúcar RB 86 7515 a 15°C, 28°C e 40°C em diferentes tempos de exposição (0, 4, 8, 12 e 24 horas) e avaliar a expressão gênica 18S. O experimento foi estabelecido em delineamento inteiramente casualizado, com seis repetições. Foram feitas as medidas de clorofila a e b, e estimada a relação a/b, e o total de clorofila nas folhas, e variáveis relacionadas aos fotossistemas. Foram medidos os valores de fluorescência inicial (F0) e máxima (Fm). De posse dessas variáveis foram estimadas as relações: Fv/Fo e a eficiência quântica potencial do fotossistema II (FSII) Fv/Fm. O RNAm foi extraído usando os mesmos tipos de folhas usadas na avaliação da fotossíntese. Altas temperaturas com exposição por 24 horas afetam a eficiência fotossintética das plantas de cana-de-açúcar. Exposição de plantas de cana-de-açúcar com 65 dias de desenvolvimento a 40 ºC por 24 horas é recomendada para avaliação da tolerância ao estresse por temperatura, visando a seleção precoce de genótipos.
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