Producción sostenible de biosurfactante por Issatchenkia orientalis UCP 1603 utilizando sustratos renovables

Autores/as

DOI:

https://doi.org/10.33448/rsd-v11i4.27174

Palabras clave:

Tensioactivo microbiano; Subproductos agroindustriales; Issatchenkia orientalis.

Resumen

Los tensioactivos biológicos son moléculas anfipáticas producidas por microorganismos y se consideran compuestos multifuncionales del siglo XXI. El presente trabajo tuvo como objetivo utilizar sustratos renovables de bajo costo para la producción económica de biosurfactante por Issatchenkia orientalis UCP 1603. Las fermentaciones se realizaron a 28°C y 150 rpm durante 72 h, utilizando subproductos agroindustriales (agua residual de yuca, licor de maíz macerado y aceite de soja post fritura) como sustratos, según un diseño factorial completo de 23 para identificar su influencia en la producción de biosurfactante. Los resultados mostraron la capacidad de la levadura para producir biosurfactante en todas las condiciones del diseño, destacándose la condición 4 debido a la mayor reducción de la tensión superficial (de 72 a 29,7 mN/m). Los análisis estadísticos evidenciaron la influencia significativa de agua residual de yuca y licor de maíz macerado en la producción del biosurfactante. Las propiedades tensoactivas de la biomolécula fueron confirmadas por prueba de parafilme e índice de emulsificación. Este estudio evidenció a I. orientalis como una levadura promisora productora de biosurfactante, con excelente capacidad y elevado potencial biotecnológico para la transformación de subproductos agroindustriales.

Biografía del autor/a

Tainã Crisia de Souza Fonseca , Federal Rural University of Pernambuco

Red de Post-graduación en Biotecnologia -RENORBIO

Adriana Ferreira de Souza, Catholic University of Pernambuco

Nucleus de Pesquisa  en Ciencias Ambientales y Biotecnologia-NPCIAMB

Patrícia Nunes dos Santos, Federal Rural University of Pernambuco

Red de Post-graduacion Programa en Biotecnologia -RENORBIO

Paulo Henrique da Silva, Federal Rural University of Pernambuco

Red de Post-graduacion Programa en Biotecnologia RENORBIO

Dayana Montero Rodriguez, Catholic University of Pernambuco

Nucleus de Pesquisa en Ciencias Ambientales y  Biotecnologia-NPCIAMB

Luiz Oliveira Costa, Catholic University of Pernambuco

Coordinador del Curso de Graduacion en Ciencias Biológicas

Citas

Andrade, R. F., Silva, T. A., Ribeaux, D. R., Rodriguez, D. M., Souza, A. F., Lima, M. A., ... & Campos-Takaki, G. M. (2018). Promising biosurfactant produced by Cunninghamella echinulata UCP 1299 using renewable resources and its application in cotton fabric cleaning process. Advances in Materials Science and Engineering, 2018. https://doi.org/10.1155/2018/1624573

Aragã, V. O., & Martins, C. M. (2014). Pichia spp. yeasts from Brazilian industrial wastewaters: Physiological characterization and potential for petroleum hydrocarbon utilization and biosurfactant production. African Journal of Microbiology Research, 8(7), 664-672.

Araújo, H. W., Andrade, R. F., Montero-Rodríguez, D., Rubio-Ribeaux, D., Alves da Silva, C. A., & Campos-Takaki, G. M. (2019). Sustainable biosurfactant produced by Serratia marcescens UCP 1549 and its suitability for agricultural and marine bioremediation applications. Microbial Cell Factories, 18(1), 1-13. https://doi.org/10.1186/s12934-018-1046-0

Cândido, T. R. S., Mendonça, R. S., Lins, U. M. D. B. L., de Souza, A. F., Rodriguez, D. M., de Campos-Takaki, G. M., & da Silva Andrade, R. F. (2022). Production of biosurfactants by Mucoralean fungi isolated from Caatinga bioma soil using industrial waste as renewable substrates. Research, Society and Development, 11(2),. http://dx.doi.org/10.33448/rsd-v11i2.25332

Dandi, N. D., Dandi, B. N. & Chaudhari, A. B. (2013) Bioprospecting of thermo- and osmo-tolerant fungi from mango pulp-peel compost for bioethanol production. Antonie van Leeuwenhoek,103, 723–736.

Galindo, H. M., Souza, A. F. Melo, E. J. V. et al. 2021. “Sustainable chitosan production by mucoralean fungi using waste post-frying oils and corn steep liquor as substrates”, International Journal of Development Research, 11, (01), 43185-43194. https://doi.org/10.37118/ijdr.20748.01.2021

Hasani, Z. P., Moghimi, H., & Hamedi, J. (2018). Biosurfactant production by Mucor circinelloides: Environmental applications and surface‐active properties. Engineering in Life Sciences, 18(5), 317-325.

Hisamatsu, M., Furubayashi, T., Karita, S., Mishima, T., Isono, N. (2006) Isolation and identification of a novel yeast fermenting ethanol under acidic conditions. Journal Applied Glycoscience, 53: 111–113.

Johny, J. M. (2013). Screening, gene sequencing and biosurfactant production from Pichia fermentans isolated from dairy effluents. IOSR J Environ Sci Toxicol Food Technol, 6(5), 2319-2402.

Joshi‐Navare, K., Singh, P. K., & Prabhune, A. A. (2014). New yeast isolate Pichia caribbica synthesizes xylolipid biosurfactant with enhanced functionality. European Journal of Lipid Science and Technology, 116(8), 1070-1079.

Katemai, W., K., Maneerat,S., Kawai,K., Kanzaki, H., Nitoda, T.’ & H-Kittikun, A. (2008), Purification and characterization of a biosurfactant produced by Issatchenkia orientalis SR4. Journal Geeral Applied Microbiology, 54, 79–82.

Kuyukina, M. S., Ivshina, I. B., Philp, J. C., Christofi, N., Dunbar, S. A., & Ritchkova, M. A. (2001). Recovery of Rhodococcus biosurfactants using methyl

tertiary-butyl ether extraction. Journal of Microbiolology Methods, 46,109-120. https://doi:10.1016/s0167-7012(01)00259-7

Kwon, Y.J., Ma, A.Z., Li, Q., Wang, F./ Zhuang, G. Q. & Liu, C. Z. (2011) Effect of lignocellulosic inhibitory compounds on growth and ethanol fermentation of newly-isolated thermotolerant Issatchenkia orientalis. Bioresource Technology, 102: 8099–8104.

Lima, R. A., Andrade, R. F., RodrÃguez, D. M., Araujo, H. W., Santos, V. P., & Campos-Takaki, G. M. (2017). Production and characterization of biosurfactant isolated from Candida glabrata using renewable substrates. African Journal of Microbiology Research, 11(6), 237-244. https://doi.org/10.5897/AJMR2016.8341

Maia, P. C., Santos, V. P., Fereira, A. S., Luna, M. A., Silva, T. A., Andrade, R. F., & Campos-Takaki, G. M. (2018). An efficient bioemulsifier-producing Bacillus subtilis UCP 0146 isolated from mangrove sediments. Colloids and Interfaces, 2(4), 58. https://doi.org/10.3390/colloids2040058

Marcelino, P. R. F., Gonçalves, F., Jimenez, I. M., Carneiro, B. C., Santos, B. B., & da Silva, S. S. (2020). Sustainable production of biosurfactants and their applications. Lignocellulosic biorefining technologies, 159-183.

Muñoz, S. S., Balbino, T. R., Alba, E. M., Barbosa, F. G., de Pier, F. T., de Almeida, A. L. M., ... & da Silva, S. S. (2022). Surfactants in biorefineries: Role, challenges & perspectives. Bioresource Technology, 345, 126477.

Nitschke, M., & Pastore, G. M. (2004). Biosurfactant production by Bacillus subtilis using cassava-processing effluent. Applied Biochemistry and Biotechnology, 112(3), 163-172.

Nwaguma, I. V., Chikere, C. B., & Okpokwasili, G. C. (2019). Isolation and molecular characterization of biosurfactant-producing yeasts from saps of Elaeis guineensis and Raphia africana. Microbiology Research Journal International, 1-12.

Oberoi, H. S., Babbar, N., Sandhu, S. K., Dhaliwal, S. S., Kaur, U., Chadha, B. S., et al. (2012) Ethanol production from alkali-treated rice straw via simultaneous saccharification and fermentation using newly isolated thermotolerant Pichia kudriavzevii HOP-1. Journal Industrial Microbiology Biotechnology, 39: 557–566.

Pele, M. A., Montero-Rodriguez, D., Rubio-Ribeaux, D., Souza, A. F., Luna, M. A., Santiago, M. F., ... & Campos-Takaki, G. M. (2018). Development and improved selected markers to biosurfactant and bioemulsifier production by Rhizopus strains isolated from Caatinga soil. African Journal of Biotechnology, 17(6), 150-157. https://doi.org/10.5897/AJB2017.16230

Poomtien, J., Thaniyavarn, J., Pinphanichakarn, P., Jindamorakot, S., & Morikawa, M. (2013). Production and characterization of a biosurfactant from Cyberlindnera samutprakarnensis JP52T. Bioscience, biotechnology, and biochemistry, 77(12), 2362-2370.

Primeia, S., Inoue, C., & Chien, M. F. (2020). Potential of biosurfactants’ production on degrading heavy oil by bacterial consortia obtained from tsunami-induced oil-spilled beach areas in Miyagi, Japan. Journal of Marine Science and Engineering, 8(8), 577.

Rahman, P. K., Mayat, A., Harvey, J. G. H., Randhawa, K. S., Relph, L. E., & Armstrong, M. C. (2019). Biosurfactants and bioemulsifiers from marine algae. In The Role of Microalgae in Wastewater Treatment (pp. 169-188). Springer, Singapore.

Rubio-Ribeaux, D., da Silva Andrade, R. F., da Silva, G. S., de Holanda, R. A., Pele, M. A., Nunes, P., ... & Campos-Takaki, G. M. (2017). Promising biosurfactant produced by a new Candida tropicalis UCP 1613 strain using substrates from renewable-resources. African Journal of Microbiology Research, 11(23), 981-991. https://doi.org/10.5897/AJMR2017.8486

Santiago, M. G., Lins, U. M. D. B. L., de Campos Takaki, G. M., da Costa Filho, L. O., & da Silva Andrade, R. F. (2021). Produção de biossurfactante por Mucor circinelloides UCP 0005 usando novo meio de cultura formulado com cascas de jatobá (Hymenaea courbaril L.) e milhocina. Brazilian Journal of Development, 7(5), 51292-51304. https://doi.org/10.34117/bjdv.v7i5.30166

Santos, D. K. F., Rufino, R. D., Luna, J. M., Santos, V. A., & Sarubbo, L. A. (2016). Biosurfactants: multifunctional biomolecules of the 21st century. International Journal of Molecular Sciences, 17(3), 401.

Shatila, F., Uyar, E., & Yalçın, H. T. (2021). Screening of biosurfactant production by Yarrowia lipolytica strains and evaluation of their antibiofilm and anti-adhesive activities against Salmonella enterica ser. enteritidis biofilms. Microbiology, 90(6), 839-847.

Singh, P., & Cameotra, S. S. (2004). Potential applications of microbial surfactants in biomedical sciences. TRENDS in Biotechnology, 22(3), 142-146.

Souza, A. F., Galindo, H. M., de Lima, M. A. B., Ribeaux, D. R., Rodríguez, D. M., da Silva Andrade, R. F., ... & de Campos-Takaki, G. M. (2020). Biotechnological strategies for chitosan production by mucoralean strains and dimorphism using renewable substrates. International Journal of Molecular Sciences, 21(12), 4286. https://doi.org/10.3390/ijms21124286

Thaniyavarn, J., Chianguthai, T., Sangvanich, P., Roongsawang, N., Washio, K., Morikawa, M., & Thaniyavarn, S. (2008). Production of sophorolipid biosurfactant by Pichia anomala. Bioscience, biotechnology, and biochemistry, 72(8), 2061-2068.

Valencia, GA, Andrade, CJD, Ienczak, JL, Monteiro, AR, & Gutiérrez, TJ (2021). Valorização dos resíduos agroalimentares. Em Biovalorização de Resíduos (pp. 111-132). Springer, Singapura.

Yalçın, H. T., Ergin‐Tepebaşı, G., & Uyar, E. (2018). Isolation and molecular characterization of biosurfactant producing yeasts from the soil samples contaminated with petroleum derivatives. Journal of basic microbiology, 58(9), 782-792.

Yaraguppi, D. A., Bagewadi, Z. K., Muddapur, U. M., & Mulla, S. I. (2020). Response surface methodology-based optimization of biosurfactant production from isolated Bacillus aryabhattai strain ZDY2. Journal of Petroleum Exploration and Production Technology, 10(6), 2483-2498.

Descargas

Publicado

14/03/2022

Cómo citar

FONSECA , T. C. de S. .; SOUZA, A. F. de .; SANTOS, P. N. dos .; SILVA, P. H. da .; RODRIGUEZ, D. M. .; COSTA, L. O. .; CAMPOS-TAKAKI, G. M. Producción sostenible de biosurfactante por Issatchenkia orientalis UCP 1603 utilizando sustratos renovables. Research, Society and Development, [S. l.], v. 11, n. 4, p. e16111427174, 2022. DOI: 10.33448/rsd-v11i4.27174. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/27174. Acesso em: 17 jul. 2024.

Número

Sección

Ingenierías