Producción de pigmento rojo por Amycolatopsis sp. UFPEDA 3422
DOI:
https://doi.org/10.33448/rsd-v11i7.29841Palabras clave:
Bio Pigmentación; Fuente de carbono; Taxonomía polifásica.Resumen
Biopigmentos rojos producidos por las actinobacterias representan un importante sustituto en la industria alimentaria a los pigmentos sintéticos que pueden causar efectos nocivos para la salud humana y el medio ambiente. En este estudio se investigó la producción de pigmentos por la cepa de actinobacteria UFPEDA 3422, identificada por taxonomía polifásica. Los parámetros de cultivo relacionados con el crecimiento y la producción de pigmentos se evaluaron en medio de cultivo sólido y en fermentación sumergida. El pigmento celular rojos se extrajo con acetato de etilo y se analizó con un espectrofotómetro UV-vis al máximo rendimiento del pigmento (34,9 AU540 nm) en medio Hickey-Tresner (HT) durante 48 horas a 37 °C. La máxima concentración de biomasa (33,4 g/L) se obtuvo después 96 horas. La taxonomía polifásica confirmó un 97,4% de similitud de la cepa UFPEDA 3422 con Amycolatopsis, un género raro capaz de producir pigmentos en diferentes substratos. Además, el microorganismo es tolerante a variaciones de temperatura, fluctuaciones de pH y concentraciones de 5% NaCl. La determinación de los parámetros de cultivo del microorganismo y la producción de pigmentos demostraron el potencial de Amycolatopsis sp. como fuente alternativa de pigmentos naturales.
Citas
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Derechos de autor 2022 Amanda Maria da Silva; Marcela de Araújo Sobral; Iranildo José Cruz Filho; Arthur Travassos de Queiroz Xavier; Mariza Severina de Lima Silva; Beate Saegesser Santos; Alexandre José da Silva Góes; Willames de Albuquerque Soares; Janete Magali Araújo; Glaucia Manoella de Souza Lima-Gomes
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