Produção de pigmento vermelho por Amycolatopsis sp. UFPEDA 3422
DOI:
https://doi.org/10.33448/rsd-v11i7.29841Palavras-chave:
Biopigmento; Fonte de carbono; Taxonomia polifásica.Resumo
Biopigmentos vermelhos produzidos por actinobactérias representam um importante substituto na indústria alimentícia aos pigmentos sintéticos, que podem causar efeitos nocivos à saúde humana e ao meio ambiente. Neste estudo, foi investigada a produção de pigmentos pela cepa de actinobacteria UFPEDA 3422, identificada por taxonomia polifásica. Os parâmetros de cultivo relacionados ao crescimento e a produção de pigmentos foram avaliados em meio de cultura sólido e fermentação submersa. O pigmento celular vermelho foi extraído com acetato de etila e analisado utilizando um espectrofotômetro UV-Vis com máxima produção de pigmento (34.9 AU540 nm) no meio Hickey-Tresner (HT) por 48 horas a 37 °C. A concentração máxima de biomassa (33.4 g/L) foi obtida após 96 horas. A taxonomia polifásica confirmou 97.4% de similaridade da cepa UFPEDA 3422 com Amycolatopsis, um gênero raro capaz de produzir pigmentos em diferentes substratos. Além disso, o micro-organismo apresenta tolerância a variações de temperatura, oscilações de pH e concentrações de 5% de NaCl. A determinação dos parâmetros de cultivo do microrganismo e produção do pigmento demonstram o potencial de Amycolatopsis sp. como uma fonte alternativa de pigmentos naturais.
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Copyright (c) 2022 Amanda Maria da Silva; Marcela de Araújo Sobral; Iranildo José Cruz Filho; Arthur Travassos de Queiroz Xavier; Mariza Severina de Lima Silva; Beate Saegesser Santos; Alexandre José da Silva Góes; Willames de Albuquerque Soares; Janete Magali Araújo; Glaucia Manoella de Souza Lima-Gomes
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