Diseño del proceso térmico de suero de queso de oveja para su valorización en producciones artesanales

Autores/as

DOI:

https://doi.org/10.33448/rsd-v11i9.31776

Palabras clave:

Suero de queso de oveja; Pasteurización lenta; Inactivación microbiana; Parámetros cinéticos; Estabilidad.

Resumen

Evaluamos la muerte térmica (62-85 ºC) de la carga microbiana nativa presente en el suero de queso de oveja (SQO) con el fin de estabilizar el suero para su posterior uso como ingrediente en producciones artesanales. Los resultados mostraron una microbiota con resistencia térmica mixta, cuya cinética de inactivación fue descrita por un modelo bifásico de primer orden. Los valores de D fueron 60,8-1,2 minutos a 62-70°C (población sensible) y 35,4-14,1 minutos a 70-75°C (población resistente). Además, se observó una población termodúrica (~1 log UFC/mL), resistente a tratamientos térmicos de hasta 85 ºC/30 min. Seleccionamos tres binomios (68, 75 y 80 ºC durante 20, 6 y 1 min, respectivamente) para estudiar el crecimiento microbiano y los cambios físicos y fisicoquímicos de la SQO durante el almacenamiento (7 ºC/ 21 días). Los resultados mostraron que el binomio de 75 °C/6 min fue la mejor opción para estabilizar la SQO, asegurando conteos microbianos aceptables hasta por 14 días de almacenamiento y, al mismo tiempo, preservando sus características físicas y fisicoquímicas. Estos resultados son fundamentales para orientar las condiciones de procesamiento de este subproducto en pequeñas propiedades vinculadas a la agricultura familiar y pueden ser aplicados directamente por investigadores y productores de queso de oveja.

Citas

AOAC International. (1999). Official Methods of Analysis. (16th ed.), AOAC Int.

Augusto, P. E. D., Tribst, A. A. L., & Cristianini, M. (2011). Thermal inactivation of Lactobacillus plantarum in a model liquid food. Journal of Food Process Engineering, 34(4), 1013-1027.

Belenkaya, S. V., Rudometov, A. P., Shcherbakov, D. N., Balabova, D. V., Kriger, a. V., Belov, A. N., Koval, A. D., & Elchaninov, V. V. (2018). Biochemical Properties of Recombinant Chymosin in Alpaca (Vicugna pacos L.). Applied Biochemistry and Microbiology, 54, 569–576.

Bhalerao, P. P. & Chakraborty, S. (2021). Integrated calculation of pasteurization time: A case study for thermal inactivation kinetics of a mixed fruit beverage. Journal of Food Process Engineering, 44 (8), e13761.

Canon, F., Nidelet, T., Guédon, E., Thierry, A., & Gagnaire, V. (2020). Understanding the Mechanisms of Positive Microbial Interactions That Benefit Lactic Acid Bacteria Co-cultures. Frontiers in Microbiology, 11, 2088.

Chapman, C. M., Gibson, G. R., & Rowland, I. (2012). In vitro evaluation of single- and multi-strain probiotics: inter-species inhibition between probiotic strains, and inhibition of pathogens. Anaerobe, 18, 405-413.

Castellano, P., Peña, N., Ibarreche, M. P., Carduza, F., Soteras, T., & Vignolo, G. (2018). Antilisterial efficacy of Lactobacillus bacteriocins and organic acids on frankfurters. Impact on sensory characteristics. Journal of Food Science and Technology, 55, 689–697.

da Silva Duarte, V., Carlot, M., Pakroo, S., Tarrah, A., Lombardi, A., Santiago, H., Corich, V., & Giacomini, A. (2020). Comparative evaluation of cheese whey microbial composition from four Italian cheese factories by viable counts and 16S rRNA gene amplicon sequencing. International Dairy Journal, 104, 104656.

da Silva, D. F., Ahrné, L., Larsen, F. H., Hougaard, A. B., & Ipsen, R. (2018). Physical and functional properties of cheese powders affected by sweet whey powder addition before or after spray drying. Powder Technology, 323, 139-148.

Dumitraşcu, L., Moschopoulou, E., Aprodu, I., Stanciu, S., Râpeanu, G., & Stǎnciuc, N. (2013). Assessing the heat induced changes in major cow and non-cow whey proteins conformation on kinetic and thermodynamic basis. Small Ruminant Research, 111(1-3), 129-138.

Elmoslemany, A. M., Keefe, G. P., Dohoo, I. R., Witchel, J. J., Stryhn, H., & Dingwell, R. T. (2010). The association between bulk tank milk analysis for raw milk quality and on-farm management practices. Preventive Veterinary Medicine, 95, 32-40.

Gabriel, A. A., Bayaga, C. L. T., Magallanes, E. A., Aba, R. P. M., & Tanguilig, K. M. N. (2020). Fates of pathogenic bacteria in time-temperature-abused and Holder-pasteurized human donor-, infant formula-, and full cream cow's milk. Food Microbiology, 89, 103450.

Johnson, M. E. (2017). A 100-year review: cheese production and quality. Journal of Dairy Science, 100 (12), 9952–9965.

Juven, B., Gordin, S., Rosenthal, I., & Laufer, A. (1981). Changes in refrigerated milk caused by Enterobacteriaceae. Journal of Dairy Science, 64, 1781-1784.

Kakagianni, M., Chatzitzika, C., Koutsoumanis,k. P., & Valdramidis, V. P. (2020). The impact of high power ultrasound for controlling spoilage by Alicyclobacillus acidoterrestris: A population and a single spore assessment. Innovative Food Science & Emerging Technologies, 64, 102405.

Kubo, M. T. K., Augusto, P. E. D., & Cristianini, M. (2013). Effect of High Pressure Homogenization (HPH) on the Physical Stability of Tomato Juice. Food Research International, 51, 170-179.

Leite Júnior, B. R. C., Tribst, A. A. L., & Cristianini, M. (2014). Proteolytic and milk-clotting activities of calf rennet processed by high pressure homogenization and the influence on the rheological behavior of the milk coagulation process. Innovative Food Science and Emerging Technology, 21, 44-49.

Lo, R., Xue, T., Weeks, M., Turner, M. S., & Bansal, N. (2016). Inhibition of bacterial growth in sweet cheese whey by carbon dioxide as determined by culture-independent community profiling. International Journal of Food Microbiology, 217, 20-28.

Macedo, A., Bilau, J., Camboias, E., & Duarte, E. C. N. F. D. (2021). Integration of Membrane Processes for By-Product Valorization to Improve the Eco-Efficiency of Small/Medium Size Cheese Dairy Plants. Foods, 10 (8), 1740.

Masiello, S. N., Kent, D., Martin,N. H., Schukken, Y. H., Wiedmann, M., & Boor, K. J. (2017). Longitudinal assessment of dairy farm management practices associated with the presence of psychrotolerant Bacillales spores in bulk tank milk on 10 New York State dairy farms. Journal of Dairy Science, 100, 8783-8795.

Mullan, W. M. A. (2019). Are we closer to understanding why viable cells of Mycobacterium avium subsp. paratuberculosis are still being reported in pasteurized milk? International Journal of Dairy Technology, 72, 332-344.

Nalepa, B., Ciesielski, S., Aljewicz, M. (2020). The microbiota of edam cheeses determined by cultivation and high-throughput sequencing of the 16S rRNA amplicon. Applied Science, 10(12), 4063.

Pala, C., Scarano, C., Venusti, M., Sardo, D., Casti, D., Cossu, F., Lamon, S., Spanu, V., Ibba, M., Marras, M., Paba, A., Spanu ,C., De Santis, E. P. L. (2016). Shelf Life Evaluation of Ricotta Fresca Sheep Cheese in Modified Atmosphere Packaging. Italian Journal of Food Safety, 5 (3), 5502.

Pereira, C. D., Henriques, M., Gomes, D., Gouveia, R., Gomez-Zavaglia, A., de Antoni, G. (2015). Fermented dairy products based on ovine cheese whey. Journal of Food Science and Technology, 52, 7401–7408.

Pflug, I. J. Selected papers on the microbiology and engineering of sterilization processes. Minneapolis: Environmental Sterilization Laboratory, 5th ed., 1988.

Ribeiro-Júnior, J. C., Tamanini, R., Oliveira, A. L. M., Alfieri, A. A., & Beloti, V. (2018). Genetic diversity of thermoduric spoilage microorganisms of milk from Brazilian dairy farms. Journal of Dairy Science, 101 (8), 6927-6936.

Thompson S. (2009) Microbiological Spoilage of High-Sugar Products. In: Sperber W. & Doyle M. Compendium of the Microbiological Spoilage of Foods and Beverages. Food Microbiology and Food Safety. Springer, New York, NY. 10.1007/978-1-4419-0826-1_11

Tribst, A. A. L., Falcade, L. T. P., Carvalho, N. S., Leite Júnior, B. R. D. C., & Oliveira, M. M. (2020). Manufacture of a fermented dairy product using whey from sheep's milk cheese: An alternative to using the main by-product of sheep's milk cheese production in small farms. International Dairy Journal, 111, 104833.

Tribst, A. A. L., Falcade, L. T. P., & Oliveira, M. M. D. (2019). Strategies for raw sheep milk storage in smallholdings: Effect of freezing or long-term refrigerated storage on microbial growth. Journal of Dairy Science, 102(6), 4960-4971.

Zotta, T., Solieri, L., Iacumin, L., Picozzi, C., & Gullo, M. (2020). Valorization of cheese whey using microbial fermentations. Applied Microbiology and Biotechnology, 104 (7), 2749-2764.

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Publicado

07/07/2022

Cómo citar

TRIBST, A. A. L.; LEITE JÚNIOR, B. R. de C. . Diseño del proceso térmico de suero de queso de oveja para su valorización en producciones artesanales. Research, Society and Development, [S. l.], v. 11, n. 9, p. e20911931776, 2022. DOI: 10.33448/rsd-v11i9.31776. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/31776. Acesso em: 17 jul. 2024.

Número

Sección

Ciencias Agrarias y Biológicas