Nanoemulsión de cinamaldehído asistida por ultrasonidos: optimización de las variables operativas, propiedades coloidales y actividad antibacteriana in vitro
DOI:
https://doi.org/10.33448/rsd-v11i9.32115Palabras clave:
Cinnamaldehído; Nanoemulsión; Ultrasonido; Actividad antibacteriana; Optimización.Resumen
Las nanoemulsiones de cinamaldehído (CNN) asistidas por ultrasonidos surgen como una alternativa energéticamente viable y potencialmente prometedora para la administración controlada de este compuesto orgánico bioactivo. Teniendo en cuenta lo anterior, el objetivo principal del estudio fue optimizar las variables operativas del proceso de producción de CNN asistida por ultrasonidos para evaluar el efecto de los elementos operativos sobre la estabilidad cinética, la composición bioactiva y la actividad antibacteriana de la CNN obtenida. Se utilizó la metodología de la superficie de respuesta (RSM), empleando un diseño compuesto central rotativo para ensamblar, cuando fuera posible, modelos polinómicos de segundo orden. El tiempo de sonicación (TS) y la amplitud de la potencia ultrasónica (AP) fueron los factores estudiados, mientras que las variables de respuesta correspondieron al diámetro hidrodinámico (DH), el índice de polidispersidad (PDI), el potencial zeta (ZP), la turbidez (T) y la composición fenólica total (TCP), respectivamente. La actividad antibacteriana in vitro de los sistemas obtenidos se llevó a cabo mediante una metodología analítica de difusión en agar de pozo. Los resultados mostraron que, independientemente del TS utilizado en el proceso, los valores más bajos de DH y T de las nanoemulsiones se obtuvieron en condiciones de baja amplitud de la potencia ultrasónica. Los valores de PDI revelaron que los NNCs eran monodispersos, con contenidos de TCP retenidos durante el proceso y una considerable actividad antibacteriana. De este modo, fue posible, a través de la MSR, proponer las condiciones óptimas de operación durante la preparación y así obtener NNCs con características fisicoquímicas que potencian su alta estabilidad cinética y el mantenimiento de la composición bioactiva y antimicrobiana durante el procesamiento.
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Derechos de autor 2022 Alane Rafaela Costa Ribeiro; Taíla Veloso de Oliveira; José Carlos Baffa Júnior; Maria do Socorro Rocha Bastos; Lais Fernanda Batista; Samiris Côcco Teixeira Teixeira; Nilda de Fátima Ferreira Soares
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