Nanoemulsão de cinamaldeído assistida por ultrassom: otimização das variáveis operacionais, propriedades coloidais e atividade antibacteriana in-vitro
DOI:
https://doi.org/10.33448/rsd-v11i9.32115Palavras-chave:
Cinamaldeído; Nanoemulsão; Ultrassom; Atividade antibacteriana; Otimização.Resumo
Nanoemulsões de cinamaldeído (NNC) assistidas por ultrassom, surgem como uma alternativa energeticamente viável e potencialmente promissora para a entrega controlada desse composto orgânico bioativo. Nesse contexto, o objetivo central do estudo consistiu na otimização das variáveis operacionais do processo de produção assistida por ultrassom de NNC, a fim de avaliar o efeito dos elementos operacionais na estabilidade cinética, composição bioativa e atividade antibacteriana das NNC obtidas. A metodologia de superfície de resposta (MSR), via delineamento composto central rotacional foi utilizada para o ajuste, quando possível, dos modelos polinomiais de segunda ordem. O tempo de sonicação (TS) e a amplitude da potência (AP) ultrassônica foram os fatores estudados, enquanto as variáveis respostas corresponderam, respectivamente ao diâmetro hidrodinâmico (DH), ao índice de polidispersidade (PDI), ao potencial zeta (PZ), a turbidez (T) e a composição fenólica total (CFT). A atividade antibacteriana in-vitro dos sistemas obtidos foi realizada por meio da metodologia analítica de difusão em ágar-poço. Os resultados mostraram que independentemente do TS utilizado no processo, os menores valores para o DH e a T das nanoemulsões foram obtidos em condições otimizadas com baixa amplitude da potência ultrassônica. Os valores de PDI revelaram que as NNC eram monodispersas, com teores de CFT preservados durante o processo de obtenção e considerável atividade antibacteriana. Logo, foi possível, por meio da MSR propor as condições operacionais ideais durante o preparo e assim obter NNC com características físico-químicas que reforçam a sua elevada estabilidade cinética e manutenção da composição bioativa e antimicrobiana durante o processamento.
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Copyright (c) 2022 Alane Rafaela Costa Ribeiro; Taíla Veloso de Oliveira; José Carlos Baffa Júnior; Maria do Socorro Rocha Bastos; Lais Fernanda Batista; Samiris Côcco Teixeira Teixeira; Nilda de Fátima Ferreira Soares
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