Evaluación del perfil fenólico y el potencial antioxidante, anticolinesterásico y anti-candida in vitro e in silico de extractos de Tapirira guianensis Aubl.
DOI:
https://doi.org/10.33448/rsd-v11i13.35378Palabras clave:
Tapirira guianensis; Antioxidante; Acetilcolinesterasa; Anti-Candida.Resumen
Se prepararon extractos polares de Tapirira guianensis Aubl. para evaluar las actividades biológicas. En todos los extractos se realizó la identificación y cuantificación de los compuestos fenólicos, la actividad antioxidante, la inhibición de la enzima acetilcolinesterasa y el potencial antifúngico. Se detectaron altos contenidos de fenoles totales en los extractos etanólico y acuoso de las hojas (237,10±5,39 mg EAG/g y 346,46±5,67 mg EAG) respectivamente. Los mismos extractos tenían un potencial de barrido de radicales libres por los métodos DPPH y ABTS+. Extracto etanólico de las hojas con CI50= 15,06±0,02 µg/mL y 16,49±0,01µg/mL) y extractos acuosos (11,54±0,01 µg/mL y 15,14±0,02 µg/mL respectivamente. En el estudio por cromatografía líquida de alto rendimiento se registraron las sustancias fenólicas: ácido gálico, rutina, ácido elágico, quercetina y apigenina en las hojas y ácido gálico y quercetina en la corteza del tallo. Los extractos etanólicos y acuosos de las hojas y de la corteza del tallo mostraron una alta potencia de inhibición de la enzima acetilcolinesterasa con valores CI50 = 13,94±0,02 µg/mL, 13,69±0,08 µg/mL y 14,79±0,20 µg/mL respectivamente. El extracto acuoso de las hojas mostró una acción anti-Candida contra las cuatro cepas del microorganismo. Los estudios in silico evidenciaron que todos los ligandos analizados presentaron interacción con la proteína SAP5 destacando el ácido elágico, la rutina y la quercetina. Con estos resultados, se confirman las actividades previamente reportadas para la especie T. guianensis, destacando los extractos polares, principalmente acuosos como fuente de sustancias antioxidantes, inhibidoras de la acetilcolinesterasa y antifúngicas.
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Derechos de autor 2022 Daniel Pereira de Oliveira; Selene Maia de Morais; Francisco Flávio da Silva Lopes; Daniela Ribeiro Alves; José Ribamar Garcez Neto; Raquel Oliveira dos Santos Fontenelle; Júlio Cesar Sousa Prado; Emmanuel da Silva Marinho; Márcia Machado Marinho; Lucas Lima Bezerra
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