Avaliação do perfil fenólico e do potencial antioxidante, anticolinesterase e anti-candida in vitro e in silico de extratos de Tapirira guianensis Aubl.
DOI:
https://doi.org/10.33448/rsd-v11i13.35378Palavras-chave:
Tapirira guianensis; Antioxidante; Acetilcolinesterase; Anti-Candida.Resumo
Extratos polares de Tapirira guianensis Aubl. foram preparados para avaliação de atividades biológicas. Identificação e quantificação de compostos fenólicos, atividade antioxidante, inibição da enzima acetilcolinesterase e potencial antifúngico foram realizados em todos os extratos. Foram detectados teores elevados de fenóis totais nos extratos etanolicos e aquosos das folhas (237,10±5,39 mg EAG/g e 346,46±5,67 mg EAG) respectivamente. Os mesmos extratos apresentavam um potencial de extração de radicais livres tanto pelo método DPPH como pelo método ABTS+. Extrato etanólico das folhas com CI50= 15,06±0,02 µg/mL e 16,49±0,01µg/mL) e extratos aquosos (11,54±0,01 µg/mL e 15,14±0,02 µg/mL respectivamente. No estudo por cromatografia líquida de alta eficiência foram registradas as substâncias fenólicas: ácido gálico, rutina, ácido elágico, quercetina e apigenina nas folhas e ácido gálico e quercetina na casca do caule. Extratos etanólicos e aquosos das folhas e casca do caule mostraram uma elevada potência de inibição da enzima acetilcolinesterase, com valores de CI50 = 13,94±0,02 µg/mL, 13,69±0,08 µg/mL e 14,79±0,20 µg/mL respectivamente. O extrato aquoso das folhas mostrou ação anti-Candida contra as quatro cepas do microrganismo. Os estudos in silico evidenciaram que todos os ligantes analisados apresentaram interação com a proteína SAP5 destacando o ácido elágico, a rutina e a quercetina. Com estes resultados, confirmam-se as atividades anteriormente relatadas para a espécie T. guianensis, destacando-se os extratos polares, principalmente aquosos como fonte de antioxidante, inibidor da acetilcolinesterase e substâncias antifúngicas.
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