Plasticidad fenotípica y caracterización de aislados de Chromobacterium del medio acuático
DOI:
https://doi.org/10.33448/rsd-v11i15.36821Palabras clave:
Resistencia antibiótica; Tolerancia a los metales; Violacein; Pigmento; Amplificación del gen rRNA 16S.Resumen
El género Chromobacterium spp. son bacilos gramnegativos, que pueden tener o no una pigmentación púrpura característica, y se aíslan principalmente del suelo, el agua y los pacientes infectados. La principal especie representativa de este género es C. violaceum, responsable de una alta tasa de mortalidad entre los infectados. El objetivo de este estudio fue caracterizar genéticamente y comparar ampliamente las características fenotípicas entre las especies del género Chromobacterium descritas en la literatura y aislamientos bacterianos. El presente estudio es una investigación experimental, en la que se realizaron pruebas de secuenciación y fenotípicas de aislados bacterianos para su comparación con especies del género Chromobacterium. Se identificaron dos cepas, CRJL01 y CRJL02 que tienen características de especies de Chromobacterium spp. Estos aislados mostraron alta resistencia a antibióticos, tolerancia y resistencia a metales, versatilidad bioquímica y fisiológica de las cepas CRJL01 y CRJL02. En la secuenciación del gen ARNr 16S, la cepa CRJL01 mostró similitud con la cepa C. piscinae. La cepa CRJL02 mostró similitud con la cepa C. subtsugae. Este trabajo es el primer informe en 40 años de Chromobacterium spp. en el Medio Oeste brasileño - Goiás, en muestras de agua. Este género aislado tiene una amplia aplicabilidad para la industria farmacéutica, alimentaria y cosmética, debido a la producción de su pigmento violeta / violeta conocido como violaceína, y su bioprospección es de gran importancia. Por lo tanto, este estudio es un punto de partida para explorar el pigmento producido.
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