Las preparaciones de Tribulus terrestris afectan los perfiles químicos de HPLC y las actividades inhibidoras de antioxidantes, lipoxigenasa y α-glucosidasa
DOI:
https://doi.org/10.33448/rsd-v11i17.38751Palabras clave:
Zygophyllaceae; Extractos de plantas; Contenido fenólico total; HPLC.Resumen
El fruto de Tribulus terrestris ha sido utilizado como medicina tradicional y popular para la prevención y tratamiento de diversas enfermedades, entre ellas la disfunción sexual, la aterosclerosis y la hipertensión. El objetivo de este estudio fue evaluar las actividades inhibidoras de antioxidantes, lipoxigenasas y α-glucosidasas de un extracto etanólico de T. terrestris brasileño y sus fracciones divididas secuencialmente en n-hexano, diclorometano, acetato de etilo y n-butanol. Las capacidades antioxidantes se determinaron mediante DPPH y ABTS eliminando radicales libres, quelando iones metálicos, reduciendo el poder y la actividad antioxidante total utilizando fosfomolibdeno. Se realizaron análisis de huellas dactilares por Cromatografía líquida de alta resolución - detector de matriz de diodos (HPLC-DAD) y cuantificación de compuestos fenólicos totales en las muestras. La fracción de diclorometano mostró el perfil químico HPLC-DAD más complejo. Las fracciones de acetato de etilo y butanol revelaron la mejor recuperación de compuestos fenólicos y flavonoides de T. terrestris. En cuanto a la actividad antioxidante, la fracción de acetato de etilo mostró mejor capacidad de captación de radicales DPPH, ABTS e hidroxilo, poder reductor, capacidad antioxidante total (TAC) y actividad inhibitoria de la α-glucosidasa que las demás fracciones. Estos resultados se correlacionaron estrechamente con los niveles de compuestos fenólicos y flavonoides. La fracción de hexano mostró el mejor poder quelante de metales y actividad inhibidora de la lipoxigenasa. El potencial antidiabético y antiinflamatorio de la T. terrestris brasileña depende de cómo se prepare.
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Derechos de autor 2022 Nelma Neylanne Pinho Muniz Oliveira; Aline Carvalho Pereira; Smail Aazza; Carolina Mesquita Germano; Rafael Marlon Alves de Assis; Simony Carvalho Mendonça; Alexandre Alves de Carvalho; José Eduardo Brasil Pereira Pinto; Suzan Kelly Vilela Bertolucci
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