Adición de cápsulas de alginato de sodio que contienen Lysinibacillus sphaericus para la autocuración de grietas en morteros

Autores/as

DOI:

https://doi.org/10.33448/rsd-v12i3.40331

Palabras clave:

Argamasa; Fisuras; Bacterias; Biomineralización; Alginato de sodio; Esferificación.

Resumen

La técnica de biomineralización para la remediación de grietas en morteros de construcción se puede realizar incorporando bacterias calcificantes a la matriz del mortero. La contracción térmica y autógena asociada con la hidratación del cemento puede ser perjudicial para la supervivencia de las esporas bacterianas. La encapsulación de las esporas en una matriz como los hidrogeles superabsorbentes, específicamente el alginato de sodio, puede brindar protección contra estas condiciones nocivas, además de servir como reservorio de agua para la actividad metabólica de las células bacterianas. Este trabajo investigó la adición de cápsulas de alginato de sodio preparadas por esferificación en morteros de construcción, con el objetivo de preservar y optimizar las propiedades reológicas, físicas y mecánicas y liberar bacterias cuando se producen fisuras. Las formulaciones de mortero con cápsulas con Lysinibacillus sphaericus se calcularon para mantener la distribución granulométrica de la formulación estándar, obteniendo así productos con excelentes propiedades reológicas, físicas y mecánicas, y permitiendo la adición de hasta un 1% de cápsulas. Se aplicó análisis de varianza multivariante en cada edad, demostrando que las formulaciones mantuvieron las mismas propiedades que la fórmula original. Luego de la formación de grietas, se pudo observar la acción de las bacterias liberadas, a través de la formación de estructuras cristalinas en las grietas. Las cápsulas se prepararon mediante un proceso sencillo y económico; además, su adición no afectó las propiedades del hormigón y protegió eficazmente a las bacterias tanto durante la mezcla y el endurecimiento como en el desprendimiento después de la fisuración.

Citas

Associação Brasileira de Normas Técnicas. (2019). Cimento Portland – Determinação da resistência à compressão de corpos de prova cilíndricos (ABNT NBR No.7215).

Associação Brasileira de Normas Técnicas. (2019). Cimento Portland – Determinação da resistência à compressão de corpos de prova prismáticos. (ABNT NBR No.16738).

Ahmed, I., Yokota, A., Yamazoe, A., & Fujiwara, T. (2007). Proposal of Lysinibacillus boronitolerans gen. nov. sp. nov., and transfer of Bacillus fusiformis to Lysinibacillus fusiformis comb. nov. and Bacillus sphaericus to Lysinibacillus sphaericus comb nov. International Journal of Systematic and Evolutionary Microbiology, 57, 1117-1125. https://doi.org/10.1099/ijs.0.63867-0

Cabrera, J. G. (1996). Deterioration of concrete due to reinforcement steel corrosion. Cement and Concrete Composites, 18(1), 47-59. https://doi.org/10.1016/0958-9465(95)00043-7

De Farias, Y. B., & Noreña, C. P. Z. (2019). Reverse encapsulation using double controlled gelification for the production of spheres with liquid light soy sauce-core. International Journal of Gastronomy and Food Science, 16, 100137. https://doi.org/10.1016/j.ijgfs.2019.100137

De Muynck, W., Debrouwer, D., De Belie, N., & Verstraete, W. (2008) Bacterial carbonate precipitation improves the durability of cementitious materials - A review. Cement and Concrete Research, 38, 1005-1014. https://doi.org/10.1016/j.cemconres.2008.03.005

Field, A., Miles, J., & Field, Z. (2012). Discovering statistics using R. Sage: London.

Isaia, G. C. (2017). Materiais de construção civil e princípios de ciência e engenharia de materiais. Ibracon: São Paulo.

Khaliq, W., & Ehsan, M. B. (2016). Crack healing in concrete using various bio influenced self-healing techniques. Construction and Building Materials, 102(1), 349-357. https://doi.org/10.1016/j.conbuildmat.2015.11.006

Lee, K. Y., & Mooney, D. J. (2012). Alginate: Properties and biomedical applications. Progress in Polymer Science, 37(1), 106-126. https://doi.org/10.1016/j.progpolymsci.2011.06.003

Lee, Y. S., & Park, W. (2018). Current challenges and future directions for bacterial self-healing concrete. Applied Microbiology and Biotechnology, 102(7), 3059-3070. https://doi.org/10.1007/s00253-018-8830-y

Oliveira, I. R., Studart, A. R., Pillegi, R. G., & Pandolfelli, V. C. (2000). Dispersão e empacotamento de partículas: princípios e aplicações em processamento cerâmico. Fazendo Arte: São Paulo.

Pungrasmi, W., Intarasoontron, J., JongvivatsakuL, P., & Likitlersuang, S. (2019). Evaluation of microencapsulation techniques for MICP bacterial spores applied in self-healing concrete. Scientific Reports, 9(1), 12484. https://doi.org/10.1038/s41598-019-49002-6

Rehman, S. K. U., et al. (2022). A Biomineralization, mechanical and durability features of bacteria-based self-healing concrete - a state of the art Review. Crystals (Basel), 12(9), 1222. https://doi.org/10.3390/cryst12091222

Roy, R., Rossi, E., Silfwerbrand, J., & Jonkers, H. (2020).Encapsulation techniques and test methods of evaluating the bacteria-based self-healing efficiency of concrete: A literature review. Nordic Concrete Research, 62(1), 63-85. https://doi.org/10.2478/ncr-2020-0006

Salman, M. M., Al-Jabbar, L. A., & Mahmod, A. K. (2021). Bacteria based self-healing concrete: A review. Journal of Engineering and Sustainable Development, 25, 33-56. https://doi.org/10.31272/jeasd/conf.2.3.4

Scott, J. E. (1968) Periodate oxidation, pKa and conformation of hexuronic acids in polyuronides and mucopolysaccharides. Biochimica et Biophysica Acta (BBA) – General Subjects, 170(2), 471-473. https://doi.org/10.1016/0304-4165(68)90040-8

Seifan, M., Samani, A. K., & Berenjian, A. (2016). Bioconcrete: next generation of self-healing concrete. Applied Microbiology and Biotechnology, 100(6), 2591-2602. https://doi.org/10.1007/s00253-016-7316-z

Severino, A. J. (2018). Metodologia do trabalho científico. Ed. Cortez: São Paulo.

Souradeep, G., & Kua, H. W. (2016). Encapsulation technology and techniques in self-healing concrete. Journal of Materials in Civil Engineering, 28(12). https://doi.org/10.1061/(ASCE)MT.1943-5533.0001687

Su, Y., Qian, C., Rui, Y., & Feng, J. (2021). Exploring the coupled mechanism of fibers and bacteria on self-healing concrete from bacterial extracellular polymeric substances (EPS). Cement and Concrete Composites, 116, 103896. https://doi.org/10.1016/j.cemconcomp.2020.103896

Trenson, G. (2017). Application of pH responsive hydrogel encapsulated bacteria for self-healing concrete. [Master’s thesis, School of Engineering]. Ghent University, Campus Repository. https://libstore.ugent.be/fulltxt/RUG01/002/367/408/RUG01-002367408_2017_0001_AC.pdf

Van Tittelboom, K., De Belie, N., De Muynck, W., & Verstraete, W. (2010). Use of bacteria to repair cracks in concrete. Cement Concrete Research, 6(1), 157-166. https://doi.org/10.1016/j.cemconres.2009.08.025

Van Tittelboom, K., De Belie, N., Van Loo, D., & Jacobs, P. (2011). Self-healing efficiency of cementitious materials containing tubular capsules filled with healing agent. Cement and Concrete Composites, 33(4), 497-505. https://doi.org/10.1016/j.cemconcomp.2011.01.004

Wang, J. Y., De Belie, N., & Verstraete, W. (2012). Diatomaceous earth as a protective vehicle for bacteria applied for self-healing concrete. Journal of Industrial Microbiology and Biotechnology, 39(4), 567-577. https://doi.org/10.1007/s10295-011-1037-1

Wang, J. Y., Van Tittelboom, K., De Belie, N., & Verstraete, W. (2012). Use of silica gel or polyurethane immobilized bacteria for self-healing concrete. Construction and Building Materials, 26(1), 532-540. https://doi.org/10.1016/j.conbuildmat.2011.06.054

Wang, J. Y., Soens, H., Verstraete, W., & De Belie, N. (2014a). Self-healing concrete by use of microencapsulated bacterial spores. Cement and Concrete Research, 56, 139-152. https://doi.org/10.1016/j.cemconres.2013.11.009

Wang, J. Y., Snoeck, D., Van Vlierberghe, S., Verstraete, W., & De Belie, N. (2014b). Application of hydrogel encapsulated carbonate precipitating bacteria for approaching a realistic self-healing in concrete. Construction and Building Materials, 68, 110-119. https://doi.org/10.1016/j.conbuildmat.2014.06.018

Wang, J., et al. (2015). Application of modified-alginate encapsulated carbonate producing bacteria in concrete: a promising strategy for crack self-healing. Frontiers in Microbiology, 6(1088),1-14. https://doi.org/10.3389/fmicb.2015.01088

Wang, J., et al. (2018). A chitosan-based pH-responsive hydrogel for encapsulation of bacteria self-healing concrete. Cement and Concrete Composites, 93, 309-322. https://doi.org/10.1016/j.cemconcomp.2018.08.007

Wang, X., Xu, J., Wang, Z., & Yao, W. (2022). Use of recycled concrete aggregates as carriers for self-healing of concrete cracks by bacteria with high urease activity. Construction and Building Materials, 337, 127581. https://doi.org/10.1016/j.conbuildmat.2022.127581

Wu, M., Johannesson, B., & Geiker, M. (2012). A review: self-healing in cementitious materials and engineered cementitious composite as a self-healing material. Construction and Building Materials, 28(1), 571-583. https://doi.org/10.1016/j.conbuildmat.2011.08.086

Zhang, X., Jin, Z., Li, M., & Qian, C. (2021). Effects of carrier on the performance of bacteria-based self-healing concrete. Construction and Building Materials, 305, 124771. https://doi.org/10.1016/j.conbuildmat.2021.124771

Descargas

Publicado

21/02/2023

Cómo citar

CRUZ, C. M. da .; MAESTRELLI, S. C.; PUGINE, S. M. P. .; SORCE, A. R.; RIGO, E. C. da S. . Adición de cápsulas de alginato de sodio que contienen Lysinibacillus sphaericus para la autocuración de grietas en morteros. Research, Society and Development, [S. l.], v. 12, n. 3, p. e4612340331, 2023. DOI: 10.33448/rsd-v12i3.40331. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/40331. Acesso em: 22 dic. 2024.

Número

Sección

Ingenierías