Caracterización estructural y funcional de HSP70 en el genoma del frijol (Phaseolous vulgaris): Un análisis in silico
DOI:
https://doi.org/10.33448/rsd-v12i12.44073Palabras clave:
Caracterización estructural; Frijol común; Proteína de choque térmico.Resumen
El frijol común (Phaseolus vulgaris L.) es la leguminosa más importante a nivel mundial, con una producción global estimada en 26,8 millones de toneladas métricas en 2016, y se cultiva por sus vainas verdes y sus semillas secas. Las plantas, en general, responden al estrés ambiental con una serie de mecanismos de adaptación fisiológicos y moleculares. Las proteínas de choque térmico (HSP) son proteínas protectoras contra una amplia gama de condiciones de estrés biótico y abiótico y son proteínas altamente conservadas que se encuentran en todos los organismos de la naturaleza. Se demostró por primera vez que la HSP70 era inducida por altas temperaturas, pero en los últimos años se ha considerado como proteínas generales que responden al estrés, que son inducidas por muchas condiciones de estrés abiótico. Otros estudios han encontrado que las HSP70 se expresan altamente en condiciones de calor, frío y estrés por sequía. Por lo tanto, se ha demostrado en algunas especies de plantas una mayor tolerancia debido a la alta expresión de HSP70. Se han utilizado varias herramientas computacionales para un análisis amplio del genoma in silico y se han utilizado para identificar nuevas proteínas y genes. Aunque las tecnologías ómicas se han vuelto significativamente populares, existen pocos estudios sobre la identificación y caracterización de HSP70 en Phaseolus vulgaris. Por lo tanto, el objetivo del presente estudio fue llevar a cabo una caracterización estructural e identificación de HSP70 en Phaseolus vulgaris utilizando métodos computacionales de todo el genoma, a saber: análisis fenético, análisis de potencial alergénico y modelado tridimensional de las secuencias.
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Derechos de autor 2023 Maria Letícia Pereira Aquino; Dalton Ferreira Matos; Genesio José do Amaral Ramos; José Alfredo dos Santos Júnior; José Valdemilson dos Santos Silva; Delma Holanda de Almeida
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